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Human Skeletal Myoblasts (HSkM), 1 vial x 106 cells - FAQs

查看更多产品信息 Human Skeletal Myoblasts (HSkM) - FAQs (A12555, A11440)

12 个常见问题解答

你们是否应用人骨骼肌成肌细胞开展任何类型的研究以重复生理条件？

请参见下方图片中显示的经生理水平的TGF-β1与IGF-1刺激的Gibco 人骨骼肌成肌细胞。TGF-β1的加入抑制了细胞的分化，而添加IGF-1（货号 PHG0075）则会减弱此效应。本次实验中使用了抗肌钙蛋白染色法。

第一代原代细胞传代时你们用什么培养基呢？

对不同的原代细胞我们使用不同的培养基和添加剂。更多详细信息可查看此处 (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).

原代细胞的群体倍增如何计算？

通过每一培养物的接种密度和收获密度来计算累计群体倍增水平。 

通常从组织中分离原代细胞的得率是多少？

这一参数随着供体年龄、组织大小、位置及我们的设备条件而发生着很大变化。某些情况下，少量组织即可分离很多细胞；而某些情况下，大量组织只能取得少量细胞。通常情况下，我们可获得30-300管细胞。

从组织中分离原代细胞需要使用酶消化吗？

是的，我们从组织中分离原代细胞需要使用酶进行消化。

何为原代细胞？

原代细胞是从活体组织（例如活检材料）中直接获取，并在体外培养的细胞。由于这些细胞经历极少的群体倍增，因此比连续（肿瘤或人工永生化的）细胞系更能代表其来源组织的主要功能成分，使原代细胞成为体内状态更具代表性的模型。
通过使用不同种属来源的原代细胞，您可了解人类与临床前测试物种之间可能存在的差异。在开展体内研究之前，可使用小鼠或大鼠细胞摸索用量并减少临床前毒理实验所需的动物数量。人细胞可用于确定从动物模型外推所得人类数据的准确性。

Have you performed any studies using the human skeletal myoblasts to replicate physiological conditions?

Please see the images below showing Gibco Human Skeletal Myoblasts responding to physiological levels of TGF-beta1 and IGF-1. TGF-beta1 addition inhibits differentiation, whereas the addition of IGF-1 (Cat. No. PHG0075) blunts this effect. The anti-troponin staining method was used.

What media do you use during the first passage of primary cells?

We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).

How is the population doubling calculated for primary cells?

Cumulative population doublings are calculated from the seeding density and harvesting density from each culture level.

What are typical primary cell yields from tissue?

It varies a lot depending on the age of donor, size of tissues, locations, and conditions arrived to our facility. Sometime a small amount of tissue gives us many cells and sometimes a large amount of tissue gives us a very small lot. Typically, we can get 30-300 vials.

Are primary cells isolated from tissue using enzymatic digestion?

Yes, our primary cells are isolated from tissue using enzymatic digestion.

What are primary cells?

Primary cells are cells taken directly from living tissue (e.g., biopsy material) and established for growth in vitro. These cells have undergone very few population doublings and are therefore more representative of the main functional component of the tissue from which they are derived in comparison to continuous (tumor or artificially immortalized) cell lines, making primary cells a more representative model for the in vivo state.

Primary cells from different species may be used, allowing you to highlight potential differences between humans and preclinical test species. Before in vivo studies, mouse or rat cells can be used to refine doses and reduce the number of animals required for preclinical toxicology. Human cells can be used to determine the accuracy of extrapolating human data from an animal model.