An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions.
AuthorsNishishita N, Muramatsu M, Kawamata S
Journal
PubMed ID25973330
Culturing human Pluripotent Stem Cells (hPSC)s in chemically defined medium and feeder-free condition can facilitate metabolome and proteome analysis of culturing cells and medium, and reduce regulatory concerns for clinical application of cells. And in addition, if hPSC are passaged and cryopreserved in single cells it also facilitates quality control ... More