引用和文献 (1)

Gibco™

CTS™ TrypLE™ Select 酶

货号	数量
A1285901	100 mL

货号 A1285901

价格（CNY)

1,030.00

100 mL

价格（CNY)

1,030.00

TrypLE™ Select CTS™, a non-animal alternative for porcine Trypsin, is a recombinant enzyme derived from microbial fermentation. The Gibco™ CTS™ product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications.

TrypLE™ Select CTS™ 是由微生物发酵获得的一种重组酶，可用作猪胰蛋白酶的非动物来源替代品。在从研究应用向临床应用过渡的过程中，GIBCO™ CTS™ 产品系列能够减少试剂验证方面的负担。

• 在从研究向临床应用的过渡过程中，GIBCO™ TrypLE™ Select ™ CTS™ 能够减少试剂验证方面的负担。
• 非动物或人源性
• 降低病毒或朊病毒污染的风险
• 清除动力学及恢复活力可与猪胰蛋白酶媲美
• 对多种细胞系具有活性，包括强附着细胞系
• 室温下（15 至 30°C）可保持稳定长达 24 个月。
• 使用专用的高压灭菌或一次性设备生产。

TrypLE™ Select CTS™ 是由微生物发酵获得的一种重组酶，可用作猪胰蛋白酶的非动物来源替代品。该材料用于从塑料器具中解离附着依赖性细胞系。无论在不含血清还是添加血清的培养系统中，TrypLE Select 都能够解离培养的细胞。该产品以 1X 储备液形式提供，使用 DPBS 配制而成，包含 1 mM EDTA。这种 rProtease 的浓度是
Invitrogen Corp. 的专有技术。

GIBCO™ CTS™ 产品质量优异，并附有相应的证明文件，如质检证明、原产地证明，可获取药品主文件授权许可证。在从科研向临床应用过渡时，GIBCO™ CTS™ 全线产品能够减少试剂验证方面的负担。

适用于研究用途或细胞、基因或组织相关产品的生产。注意：不适用于向人或动物进行直接给药

用于研究或生产基于细胞、基因或者组织的产品。注意：不可直接作用于人类或动物给药。

细胞类型哺乳动物细胞

最大浓度1 X

适用于（应用）临床研究、转化研究

产品规格瓶

生产质量ISO 13485, MDSAP, FDA-registered, 21 CFR 820

数量100 mL

有效期24 个月

运输条件室温

分类无动物来源

产品类型细胞培养解离试剂

无菌无菌过滤

不加添加剂无酚红

Unit SizeEach

内容与储存

在室温下储存。

常见问题解答 (FAQ)

Gibco TrypLE Express与Gibco TrypLE Select两款产品间的区别是什么？

这两款产品均含有重组蛋白酶，这是一款类似胰酶的非动物源性产品，可用于解离贴壁细胞。这款产品是利用微生物生产的重组酶，可替代猪源的胰酶产品。
Express与Select产品在生产与测试方法，以及提供的文件方面都有差别。TrypLE Select主要面向工业市场，通过了内毒素和酶活性的出厂测试。同时该产品也具有药物主文件记录。这款产品使用AOF专用设备进行生产，指定用途为研究级应用及进一步的细胞培养生产。
TrypLEExpress未使用专门设备进行生产，用途仅为科学研究。不适用于诊断操作。
如需阅读更多关于TrypLE产品的信息以及使用该试剂所需的细胞解离时间，请点击here(https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).

你们提供哪些用于解离细胞的试剂？这些产品之间的主要区别是什么？

请通过此选择列表(https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html)来比较我们的细胞解离试剂。

What is CTS?

The Gibco Cell Therapy Systems (CTS) portfolio of cell and gene therapy products are GMP manufactured, safety tested, and backed by regulatory documentation to support your transition from discovery through clinical and commercial manufacturing. Through our CTS solutions, we are committed to helping customers streamline therapeutic development, minimize risk, and ease the burden on their quality systems. Learn more here.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I assess residual TrypLE reagent remaining after my downstream processing steps?

We offer the Residual TrypLE ELISA Kit (Cat. No. A6533101) that can specifically detect the recombinant protease (rProtease) in TrypLE reagent.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is there an upper limit for the amount of time cells can be incubated with TrypLE Express Enzyme, TrypLE Select Enzyme, or CTS TrypLE Select Enzyme for dissociation?

There is no upper limit for TrypLE enzyme incubation. You can incubate your cells with TrypLE longer to detach cells. 30-45 min at 37 degrees C will be usually fine. Or you can consider using TrypLE Select Enzyme (10X) (Cat. No. A1217701 or A1217702 or A1217703) or CTS TrypLE Select Enzyme (Cat. No. A4738001) depending upon the application.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all CTS™ TrypLE™ Select 酶 FAQs

引用和文献 (1)

引用和文献

Abstract

An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions.

Authors:Nishishita N, Muramatsu M, Kawamata S

Journal:

PubMed ID:25973330

Culturing human Pluripotent Stem Cells (hPSC)s in chemically defined medium and feeder-free condition can facilitate metabolome and proteome analysis of culturing cells and medium, and reduce regulatory concerns for clinical application of cells. And in addition, if hPSC are passaged and cryopreserved in single cells it also facilitates quality control ... More