Custom Primer - FAQs

In the TaqMan reagent kits, what is the purpose of the Passive Reference dye?

The Passive Reference Dye, ROX, is used to normalize real-time PCR reactions. ROX dye provides an internal reference to which the reporter dye signal can be normalized, which is necessary to correct for fluorescent fluctuations due to changes in concentration, volume or light source intensity. Normalization of the reporter dye signal results in increased data precision.

ROX dye performs the following specific functions:

- Normalizes most fluorescent fluctuations, including variation in intensity from the light source
- Compensates for well-to-well volume variations
- Regulates minor volume differences and changes in concentration
- Allows for high precision

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.