Antagonistic effects of cofilin, beryllium fluoride complex, and phalloidin on subdomain 2 and nucleotide-binding cleft in F-actin.
AuthorsMuhlrad A, Ringel I, Pavlov D, Peyser YM, Reisler E
JournalBiophys J
PubMed ID16997870
'Cofilin/ADF, beryllium fluoride complex (BeFx), and phalloidin have opposing effects on actin filament structure and dynamics. Cofilin/ADF decreases the stability of F-actin by enhancing disorder in subdomain 2, and by severing and accelerating the depolymerization of the filament. BeFx and phalloidin stabilize the subdomain 2 structure and decrease the critical ... More
Dynamic polymorphism of single actin molecules in the actin filament.
AuthorsKozuka J, Yokota H, Arai Y, Ishii Y, Yanagida T
JournalNat Chem Biol
PubMed ID16415860
'Actin filament dynamics are critical in cell motility. The structure of actin filament changes spontaneously and can also be regulated by actin-binding proteins, allowing actin to readily function in response to external stimuli. The interaction with the motor protein myosin changes the dynamic nature of actin filaments. However, the molecular ... More
Synthesis and characterization of conjugates formed between periodate-oxidized ribonucleotides and amine-containing fluorophores.
AuthorsHileman RE, Parkhurst KM, Gupta NK, Parkhurst LJ
JournalBioconjug Chem
PubMed ID7849074
The synthesis and purification of new fluorescently labeled derivatives of GDP and ATP are described. The fluorescent groups are coupled initially through amine-containing linker arms to periodate-oxidized nucleotides. Reduction of the initial product yields primarily a six-membered morpholine-like ring. Fluorescein-labeled GDP, rhodamine-labeled GDP, and fluorescein-labeled ATP were characterized by absorbance ... More
The mitogen-activated protein kinase kinase kinase dual leucine zipper-bearing kinase (DLK) acts as a key regulator of keratinocyte terminal differentiation.
AuthorsRobitaille H, Proulx R, Robitaille K, Blouin R, Germain L
JournalJ Biol Chem
PubMed ID15695824
In the skin, epithelial cells undergo a terminal differentiation program leading to the formation of the stratum corneum. Although it is expected that the last phases of this process must be tightly regulated since it results in cell death, the signaling pathways involved in this induction remain ill defined. We ... More
Orientation of actin monomer in the F-actin filament: radial coordinate of glutamine-41 and effect of myosin subfragment 1 binding on the monomer orientation.
AuthorsKasprzak AA, Takashi R, Morales MF
JournalBiochemistry
PubMed ID3166995
We have employed the method of radial distance measurements in order to orient the actin monomer in the F-actin filament. This method utilizes fluorescence resonance energy transfer measurements of the distance between two equivalent chemical points located on two different monomers. The interprobe distance obtained this way is used to ... More
Trifunctional chemical probes for the consolidated detection and identification of enzyme activities from complex proteomes.
AuthorsAdam GC, Sorensen EJ, Cravatt BF
JournalMol Cell Proteomics
PubMed ID12438565
Chemical probes that covalently modify the active sites of enzymes in complex proteomes are useful tools for identifying enzyme activities associated with discrete (patho) physiological states. Researchers in proteomics typically use two types of activity-based probes to fulfill complementary objectives: fluorescent probes for rapid and sensitive target detection and biotinylated ... More