The p42/p44 MAP kinase pathway prevents apoptosis induced by anchorage and serum removal.
AuthorsLe Gall M,Chambard JC,Breittmayer JP,Grall D,Pouysségur J,Van Obberghen-Schilling E
JournalMolecular biology of the cell
PubMed ID10712523
Anchorage removal like growth factor removal induces apoptosis. In the present study we have characterized signaling pathways that can prevent this cell death using a highly growth factorâ and anchorage-dependent line of lung fibroblasts (CCL39). After anchorage removal from exponentially growing cells, annexin V-FITC labeling can be detected after 8 ... More
Authors
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PubMed ID18258751
Authors
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PubMed ID10891486
Strategies for phenotyping apoptotic peripheral human lymphocytes comparing ISNT, annexin-V and 7-AAD cytofluorometric staining methods.
AuthorsLecoeur H,Ledru E,Prévost MC,Gougeon ML
JournalJournal of immunological methods
PubMed ID9461328
The present article compares the reliability of four previously described cytofluorometric methods of apoptosis quantification for phenotyping apoptotic human lymphocytes. Each of these assays detects distinct cellular alterations of the apoptotic process. Alteration in plasma membrane integrity can be evaluated following 7-AAD incorporation and the translocation of phosphatidylserine from the ... More
Analysis of ethanol effects on corneal epithelium.
AuthorsOh JY, Yu JM, Ko JH,
JournalInvest Ophthalmol Vis Sci
PubMed ID23674759
Ethanol is widely used in ocular surface surgeries and for the treatment of corneal diseases. However, ethanol is a toxic agent that is related to the development of a number of alcohol-related diseases. Despite the common use of ethanol for therapeutic purposes in ophthalmology, effects of ethanol on the ocular ... More
Tamoxifen but not 4-hydroxytamoxifen initiates apoptosis in p53(-) normal human mammary epithelial cells by inducing mitochondrial depolarization.
AuthorsDietze EC, Caldwell LE, Grupin SL, Mancini M, Seewaldt VL
JournalJ Biol Chem
PubMed ID11094056
'Despite the widespread clinical use of tamoxifen as a breast cancer prevention agent, the molecular mechanism of tamoxifen chemoprevention is poorly understood. Abnormal expression of p53 is felt to be an early event in mammary carcinogenesis. We developed an in vitro model of early breast cancer prevention to investigate how ... More
Cycloheximide-induced T-cell death is mediated by a Fas-associated death domain-dependent mechanism.
AuthorsTang D, Lahti JM, Grenet J, Kidd VJ
JournalJ Biol Chem
PubMed ID10066786
'Cycloheximide (CHX) can contribute to apoptotic processes, either in conjunction with another agent (e.g. tumor necrosis factor-alpha) or on its own. However, the basis of this CHX-induced apoptosis has not been clearly established. In this study, the molecular mechanisms of CHX-induced cell death were examined in two different human T-cell ... More
Targeting of the c-Abl tyrosine kinase to mitochondria in the necrotic cell death response to oxidative stress.
AuthorsKumar S, Bharti A, Mishra NC, Raina D, Kharbanda S, Saxena S, Kufe D
JournalJ Biol Chem
PubMed ID11350980
'The ubiquitously expressed c-Abl tyrosine kinase is activated in the response of cells to genotoxic and oxidative stress. The present study demonstrates that reactive oxygen species (ROS) induce targeting of c-Abl to mitochondria. We show that ROS-induced localization of c-Abl to mitochondria is dependent on activation of protein kinase C ... More
Selective cell targeting with light-absorbing microparticles and nanoparticles.
AuthorsPitsillides CM, Joe EK, Wei X, Anderson RR, Lin CP
JournalBiophys J
PubMed ID12770906
'We describe a new method for selective cell targeting based on the use of light-absorbing microparticles and nanoparticles that are heated by short laser pulses to create highly localized cell damage. The method is closely related to chromophore-assisted laser inactivation and photodynamic therapy, but is driven solely by light absorption, ... More
Inhibition of tumor necrosis factor alpha-mediated NFkappaB activation and leukocyte adhesion, with enhanced endothelial apoptosis, by G protein-linked receptor (TP) ligands.
AuthorsAshton AW, Ware GM, Kaul DK, Ware JA
JournalJ Biol Chem
PubMed ID12517920
'Tumor necrosis factor (TNF) alpha is a critical mediator of inflammation; however, TNFalpha is rarely released alone and the "cross-talk" between different classes of inflammatory mediators is largely unexplored. Thromboxane A(2) (TXA(2)) is released during I/R injury and exerts its effects via a G protein-linked receptor (TP). In this study, ... More
The ubiquitin-like protein FAT10 forms covalent conjugates and induces apoptosis.
AuthorsRaasi S, Schmidtke G, Groettrup M
JournalJ Biol Chem
PubMed ID11445583
'FAT10 is a ubiquitin-like protein that is encoded in the major histocompatibility complex class I locus and is synergistically inducible with interferon-gamma and tumor necrosis factor alpha. The molecule consists of two ubiquitin-like domains in tandem arrangement and bears a conserved diglycine motif at its carboxyl terminus commonly used in ... More
Candida albicans phospholipomannan promotes survival of phagocytosed yeasts through modulation of bad phosphorylation and macrophage apoptosis.
AuthorsIbata-Ombetta S, Idziorek T, Trinel PA, Poulain D, Jouault T
JournalJ Biol Chem
PubMed ID12551950
'The surface of the pathogenic yeast Candida albicans is coated with phospholipomannan (PLM), a phylogenetically unique glycolipid composed of beta-1,2-oligomannosides and phytoceramide. This study compared the specific contribution of PLM to the modulation of signaling pathways linked to the survival of C. albicans in macrophages in contrast to Saccharomyces cerevisiae. ... More
Cytochrome c-mediated apoptosis in cells lacking mitochondrial DNA. Signaling pathway involving release and caspase 3 activation is conserved.
AuthorsJiang S, Cai J, Wallace DC, Jones DP
JournalJ Biol Chem
PubMed ID10514472
'Mitochondria serve as a pivotal component of the apoptotic cell death machinery. However, cells that lack mitochondrial DNA (rho(0) cells) retain apparently normal apoptotic signaling. In the present study, we examined mitochondrial mechanisms of apoptosis in rho(0) osteosarcoma cells treated with staurosporine. Immunohistochemistry revealed that rho(0) cells maintained a normal ... More
Temperature-dependent arrest of neutrophil apoptosis. Failure of Bax insertion into mitochondria at 15 degrees C prevents the release of cytochrome c.
AuthorsPryde JG, Walker A, Rossi AG, Hannah S, Haslett C
JournalJ Biol Chem
PubMed ID10896657
'Apoptosis is essential for the resolution of neutrophilic inflammation. To define the mechanisms triggering the execution phase of apoptosis we developed and utilized a model in which culture of human neutrophils at 15 degrees C for 20 h arrested apoptosis and subsequent warming to 37 degrees C triggered a synchronous ... More
Expression of mitochondrial Apo2.7 molecules and caspase-3 activation in human lymphocytes treated with the ribosome-inhibiting mistletoe lectins and the cell membrane permeabilizing viscotoxins.
AuthorsBüssing A, Vervecken W, Wagner M, Wagner B, Pfüller U, Schietzel M
JournalCytometry
PubMed ID10486525
'BACKGROUND: It is unclear whether expression of newly described mitochondrial Apo2.7 molecules (7A6 antigen) is specific for apoptosis or may also occur in necrosis. METHODS: We incubated human lymphocytes with the apoptosis-inducing mistletoe lectin (ML) I and the cell membrane-permeabilizing viscotoxins (VT), and measured cell death-associated changes by flow cytometry. ... More
A rapid single-laser flow cytometric method for discrimination of early apoptotic cells in a heterogenous cell population.
AuthorsHerault O, Colombat P, Domenech J, Degenne M, Bremond JL, Sensebe L, Bernard MC, Binet C
JournalBr J Haematol
PubMed ID10086791
'A recently reported cytometric method described the possibility of discriminating apoptotic from necrotic cells using FITC-labelled annexin V and propidium iodide (PI). Nevertheless, the brightness of PI-staining and its extensive spectral emission overlap with phycoerythrin (PE) does not permit the study of a subset of a heterogenous cell population with ... More
An antisense oligonucleotide to 1-cys peroxiredoxin causes lipid peroxidation and apoptosis in lung epithelial cells.
AuthorsPak JH, Manevich Y, Kim HS, Feinstein SI, Fisher AB
JournalJ Biol Chem
PubMed ID12372839
'1-cys peroxiredoxin (1-cysPrx), a member of the peroxiredoxin superfamily, reduces phospholipid hydroperoxides as well as organic peroxides and H(2)O(2). To determine the physiological function(s) of 1-cysPrx, we have used an antisense strategy to suppress endogenous 1-cysPrx in L2 cells, a rat lung epithelial cell line. A 25-base antisense morpholino oligonucleotide ... More
Protease involvement in fodrin cleavage and phosphatidylserine exposure in apoptosis.
AuthorsVanags DM, Pörn-Ares MI, Coppola S, Burgess DH, Orrenius S
JournalJ Biol Chem
PubMed ID8940103
'A detailed kinetic analysis of three extranuclear end points of apoptosis, phosphatidylserine exposure, alpha-fodrin degradation, and plasma membrane blebbing, was performed and compared with nuclear fragmentation and the activation of the interleukin-1beta-converting enzyme (ICE)-like proteases in Jurkat T lymphocytes stimulated by anti-Fas monoclonal antibody (anti-Fas mAb) and in monocytic U937 ... More
Quantitative measurement of mast cell degranulation using a novel flow cytometric annexin-V binding assay.
AuthorsDemo SD, Masuda E, Rossi AB, Throndset BT, Gerard AL, Chan EH, Armstrong RJ, Fox BP, Lorens JB, Payan DG, Scheller RH, Fisher JM
JournalCytometry
PubMed ID10404150
'BACKGROUND: Mast cells are primary mediators of allergic inflammation. Antigen-mediated crosslinking of their cell surface immunoglobulin E (IgE) receptors results in degranulation and the release of proinflammatory mediators including histamine, tumor necrosis factor-alpha, and leukotrienes. METHODS: Mast cells were stimulated to degranulate by using either IgE crosslinking or ionophore treatment. ... More
Diazoxide triggers cardioprotection against apoptosis induced by oxidative stress.
AuthorsIchinose M, Yonemochi H, Sato T, Saikawa T
JournalAm J Physiol Heart Circ Physiol
PubMed ID12623783
'Although mitochondrial ATP-sensitive potassium (mitoK(ATP)) channels have been reported to reduce the extent of apoptosis, the critical timing of mitoK(ATP) channel opening required to protect myocytes against apoptosis remains unclear. In the present study, we examined whether the mitoK(ATP) channel serves as a trigger of cardioprotection against apoptosis induced by ... More
Flex-Hets differentially induce apoptosis in cancer over normal cells by directly targeting mitochondria.
AuthorsLiu T, Hannafon B, Gill L, Kelly W, Benbrook D
JournalMol Cancer Ther
PubMed ID17575110
'Flex-Het drugs induce apoptosis in multiple types of cancer cells, with little effect on normal cells. This apoptosis occurs through the intrinsic mitochondrial pathway accompanied by generation of reactive oxygen species (ROS). The objective of this study was to determine if direct or indirect targeting of mitochondria is responsible for ... More
Apoptosis in shed human corneal cells.
AuthorsEstil S, Primo EJ, Wilson G
JournalInvest Ophthalmol Vis Sci
PubMed ID11006225
'PURPOSE: To determine whether shear forces applied to the corneal epithelium by the repeated insertion and removal of a hydrogel contact lens alter the size and number of cells removed and to determine the contribution of apoptosis to this process. METHODS; Human corneal cells were collected from eight healthy subjects ... More
Hidden effects of cryopreservation on quality of human spermatozoa.
AuthorsGlander HJ, Schaller J
JournalCell Tissue Bank
PubMed ID15256959
'The effects of cryopreservation on two characteristics of human spermatozoa were investigated: the early phases of disturbed plasma membrane function and the activity of enzymes in intact spermatozoa. The membrane function was detected by means of the calcium-dependent binding of fluorescein isothiocyanate (FITC)-conjugated Annexin V to sperm plasma membranes. Annexin ... More
Tethered polymer-supported planar lipid bilayers for reconstitution of integral membrane proteins: silane-polyethyleneglycol-lipid as a cushion and covalent linker.
AuthorsWagner ML, Tamm LK
JournalBiophys J
PubMed ID10969002
'There is increasing interest in supported membranes as models of biological membranes and as a physiological matrix for studying the structure and function of membrane proteins and receptors. A common problem of protein-lipid bilayers that are directly supported on a hydrophilic substrate is nonphysiological interactions of integral membrane proteins with ... More
The NF-kappa B cascade is important in Bcl-xL expression and for the anti-apoptotic effects of the CD28 receptor in primary human CD4+ lymphocytes.
AuthorsKhoshnan A, Tindell C, Laux I, Bae D, Bennett B, Nel AE
JournalJ Immunol
PubMed ID10925251
'We explored the role of the NF-kappa B pathway in the survival of primary human CD4+ T lymphocytes during CD28 costimulation. Transduction of proliferating CD4+ T cells with a tetracycline-regulated retrovirus encoding for a dominant-interfering, degradation-resistant I-kappaBalpha (inhibitor of kappa B alpha factor) mutant induced apoptosis. Using DNA arrays, we ... More
CCAAT/enhancer-binding protein delta regulates mammary epithelial cell G0 growth arrest and apoptosis.
'CCAAT/enhancer-binding proteins (C/EBPs) are a highly conserved family of DNA-binding proteins that regulate cell-specific growth, differentiation, and apoptosis. Here, we show that induction of C/EBPdelta gene expression during G0 growth arrest is a general property of mammary-derived cell lines. C/EBPdelta is not induced during G0 growth arrest in 3T3 or ... More
Molecular mechanisms of erythrophagocytosis. Characterization of the senescent erythrocytes that are phagocytized by macrophages.
'We have recently developed a flow cytometric assay for the quantitation of erythrophagocytosis, using PKH 26-labeled erythrocytes as the target cells. Using this assay we have shown that there is extensive phagocytosis of desialylated erythrocytes. Furthermore, we have demonstrated that it is the densest population of erythrocytes obtained on a ... More
Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death.
AuthorsWei MC, Zong WX, Cheng EH, Lindsten T, Panoutsakopoulou V, Ross AJ, Roth KA, MacGregor GR, Thompson CB, Korsmeyer SJ
JournalScience
PubMed ID11326099
'Multiple death signals influence mitochondria during apoptosis, yet the critical initiating event for mitochondrial dysfunction in vivo has been unclear. tBID, the caspase-activated form of a "BH3-domain-only" BCL-2 family member, triggers the homooligomerization of "multidomain" conserved proapoptotic family members BAK or BAX, resulting in the release of cytochrome c from ... More
Annexin V, the regulator of phosphatidylserine-catalyzed inflammation and coagulation during apoptosis.
AuthorsReutelingsperger CP, van Heerde WL
JournalCell Mol Life Sci
PubMed ID9230931
'Annexin V belongs to a family of phospholipid binding proteins, the Annexins. It binds in the presence of Ca(2+)-ions with high affinity to negatively charged phospholipids like phosphatidylserine (PS). On the basis of its protein structure and biological activity Annexin V is considered as a protein exhibiting its hitherto unknown ... More
Surface blebs on apoptotic cells are sites of enhanced procoagulant activity: implications for coagulation events and antigenic spread in systemic lupus erythematosus.
AuthorsCasciola-Rosen L, Rosen A, Petri M, Schlissel M
JournalProc Natl Acad Sci U S A
PubMed ID8643681
'The restriction of phosphatidylserine (PtdSer) to the inner surface of the plasma membrane bilayer is lost early during apoptosis. Since PtdSer is a potent surface procoagulant, and since there is an increased incidence of coagulation events in patients with systemic lupus erythematosus (SLE) who have anti-phospholipid antibodies, we addressed whether ... More
A comparative study of different methods for the assessment of apoptosis and necrosis in human eosinophils.
AuthorsWalsh GM, Dewson G, Wardlaw AJ, Levi-Schaffer F, Moqbel R
JournalJ Immunol Methods
PubMed ID9776585
'Eosinophils, prominent cells in asthmatic inflammation, undergo apoptosis or programmed cell death following deprivation of contact with survival-promoting cytokines such as IL-5 and GM-CSF. The aim of this study was to assess a number of techniques for the quantification of apoptosis in human eosinophils cultured with or without IL-5 or ... More
Phagocytosis of apoptotic cells by human macrophages: analysis by multiparameter flow cytometry.
AuthorsJersmann HP, Ross KA, Vivers S, Brown SB, Haslett C, Dransfield I
JournalCytometry A
PubMed ID12500300
'BACKGROUND: Phagocytic removal of apoptotic cells is an important regulatory event in development, tissue homoeostasis, and inflammation. There are several methodologic problems with most in vitro studies of the molecular mechanisms of apoptotic cell phagocytosis. First, cell loss occurs during rigorous washing of adherent macrophages required to ensure removal of ... More
Endostatin induces endothelial cell apoptosis.
AuthorsDhanabal M, Ramchandran R, Waterman MJ, Lu H, Knebelmann B, Segal M, Sukhatme VP
JournalJ Biol Chem
PubMed ID10206987
'Endostatin, a carboxyl-terminal fragment of collagen XVIII, has been shown to regress tumors in mice. In this study, we have analyzed the mechanism of endostatin action on endothelial cells and nonendothelial cells. Endostatin treatment of cow pulmonary artery endothelial cells caused apoptosis, as demonstrated by three methods, annexin V-fluorescein isothiocyanate ... More
Detection of altered membrane phospholipid asymmetry in subpopulations of human red blood cells using fluorescently labeled annexin V.
AuthorsKuypers FA, Lewis RA, Hua M, Schott MA, Discher D, Ernst JD, Lubin BH
JournalBlood
PubMed ID8562945
'The phospholipids of the human red cell are distributed asymmetrically in the bilayer of the red cell membrane. In certain pathologic states, such as sickle cell anemia, phospholipid asymmetry is altered. Although several methods can be used to measure phospholipid organization, small organizational changes have been very difficult to assess. ... More
A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V.
AuthorsVermes I, Haanen C, Steffens-Nakken H, Reutelingsperger C
JournalJ Immunol Methods
PubMed ID7622868
'In the early stages of apoptosis changes occur at the cell surface, which until now have remained difficult to recognize. One of these plasma membrane alterations is the translocation of phosphatidylserine (PS) from the inner side of the plasma membrane to the outer layer, by which PS becomes exposed at ... More
312-nanometer ultraviolet B light (narrow-band UVB) induces apoptosis of T cells within psoriatic lesions.
AuthorsOzawa M, Ferenczi K, Kikuchi T, Cardinale I, Austin LM, Coven TR, Burack LH, Krueger JG
JournalJ Exp Med
PubMed ID9989986
'Narrow-band (312 nm) ultraviolet B light (UVB) is a new form of therapy for psoriasis, but its mechanism of action is unknown. In a bilateral comparison clinical study, daily exposure of psoriatic plaques to broad-band UVB (290-320 nm) or 312-nm UVB depleted T cells from the epidermis and dermis of ... More
Stress-induced fractal rearrangement of the endothelial cell cytoskeleton causes apoptosis.
'BACKGROUND: Apoptosis, a mechanism of cell death prominent in critical illnesses including disseminated inflammation and multiorgan dysfunction syndrome, is characterized by morphologic changes including cell shrinkage, condensation of organelles, blebbing, and chromatin fragmentation. These phenomena suggest substantial changes in cytoskeletal structure. We hypothesized that stress-induced apoptosis in endothelial cells is, ... More
CpG oligodeoxynucleotide protection in polymicrobial sepsis is dependent on interleukin-17.
'CpG oligodeoxynucleotides (ODNs) may prevent mortality from infection. We have identified a therapeutic benefit in treating sepsis with phosphorothioate ODN sequences containing the CpG motif. Sepsis was induced in rats by cecal ligation and puncture (CLP), and treatment with CpG ODNs reduced sepsis mortality from 80% to 15% during a ... More
Ephrin-B1 is critical in T-cell development.
AuthorsYu G, Mao J, Wu Y, Luo H, Wu J
JournalJ Biol Chem
PubMed ID16476740
'Eph kinases are the largest family of receptor tyrosine kinases, and their ligands, ephrins (EFNs), are also cell surface molecules. In this study, we investigated the role of EFNB1 and the Ephs it interacts with (collectively called EFNB1 receptors) in mouse T-cell development. In the thymus, CD8 single positive (SP) ... More
Radiation-induced reactive oxygen species formation prior to oxidative DNA damage in human peripheral T cells.
AuthorsOgawa Y, Kobayashi T, Nishioka A, Kariya S, Hamasato S, Seguchi H, Yoshida S
JournalInt J Mol Med
PubMed ID12525868
'Previously, we demonstrated that human peripheral T lymphocytes revealed early apoptotic changes (annexin V-positive) and late apoptotic changes (propidium iodide-positive), at 13 and 24 h, respectively, after irradiation of 5 Gy. Changes in mitochondrial membrane potential were observed at 10 h after irradiation of 5 Gy. Subsequently, mitochondrial cytochrome c-release ... More
Apoptotic activity in stored human platelets.
AuthorsPerrotta PL, Perrotta CL, Snyder EL
JournalTransfusion
PubMed ID12662287
'BACKGROUND: Platelets possess some of the machinery required for apoptotic cell death. However, disruption of mitochondria function, implicated in several models of cell death, has not been extensively studied in platelets. Mitochondrial viability and several other measures of apoptotic death in stored and experimentally stressed platelets were evaluated. MATERIALS AND ... More
Multiparameter detection of apoptosis using red-excitable SYTO probes.
'Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent ... More
Hydralazine, but not captopril, decreases free radical production and apoptosis in neurons and thymocytes.
AuthorsJohnson P, Wei Y, Huentelman MJ, Peters CM, Boldyrev AA
JournalFree Radic Res
PubMed ID9684984
'The effects of captopril and hydralazine, two commonly used antihypertensive drugs, on free radical generation and the onset of apoptosis in neuron and thymocyte preparations from 10-12 day old rats have been studied. Apoptosis was induced in neurons by kainate or N-methyl-D-aspartate and in thymocytes by heat shock. Intracellular free ... More
Cigarette smoke extract induces oxidative stress and apoptosis in human lung fibroblasts.
AuthorsCarnevali S, Petruzzelli S, Longoni B, Vanacore R, Barale R, Cipollini M, Scatena F, Paggiaro P, Celi A, Giuntini C
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID12547733
'Cigarette smoke is a mixture of chemicals having direct and/or indirect toxic effects on different lung cells. We investigated the effect of cigarette smoke on human lung fibroblasts (HFL-1) oxidation and apoptosis. Cells were exposed to various concentrations (1, 5, and 10%) of cigarette smoke extract (CSE) for 3 h, ... More
Effects of extracellular ATP and adenosine on different thymocyte subsets: possible role of ATP-gated channels and G protein-coupled purinergic receptor.
AuthorsApasov SG, Koshiba M, Chused TM, Sitkovsky MV
JournalJ Immunol
PubMed ID9164924
'To explore the possible role of purinergic receptors in thymocyte development and in pathogenesis of adenosine deaminase SCID, we studied effects of extracellular adenosine triphosphate (ATP(ext)) and adenosine on TCR- and steroid hormone-triggered processes in mouse thymocytes. Reverse transcriptase-PCR analysis confirms the mRNA expression of several types of purinergic receptors, ... More
The role of ARK in stress-induced apoptosis in Drosophila cells.
AuthorsZimmermann KC, Ricci JE, Droin NM, Green DR
JournalJ Cell Biol
PubMed ID11901172
'The molecular mechanisms of apoptosis are highly conserved throughout evolution. The homologs of genes essential for apoptosis in Caenorhabditis elegans and Drosophila melanogaster have been shown to be important for apoptosis in mammalian systems. Although a homologue for CED-4/apoptotic protease-activating factor (Apaf)-1 has been described in Drosophila, its exact function ... More
Poly(ADP-ribose) synthetase activation mediates mitochondrial injury during oxidant-induced cell death.
AuthorsVirág L, Salzman AL, Szabó C
JournalJ Immunol
PubMed ID9759901
'Reactive oxidant species are important mediators of tissue injury in shock, inflammation, and reperfusion injury. The actions of a number of these oxidants (e.g., hydroxyl radical and peroxynitrite, a reactive oxidant produced by the reaction of nitric oxide and superoxide) are mediated in part by the activation of the nuclear ... More
Induction of necrosis by zinc in prostate carcinoma cells and identification of proteins increased in association with this induction.
AuthorsIguchi K, Hamatake M, Ishida R, Usami Y, Adachi T, Yamamoto H, Koshida K, Uchibayashi T, Hirano K
JournalEur J Biochem
PubMed ID9654077
'Zinc exhibits inhibitory effects on apoptosis, and a deficiency in this metal generally causes this type of cell death to occur. In the present study, we found that exposure to zinc results in necrosis of prostate carcinoma cells. When zinc acetate was added to LNCaP or PC-3 cells in monolayer ... More
Membrane depolarization and depletion of intracellular calcium stores are associated with delay of apoptosis in human neutrophils.
AuthorsChacon-Cruz E, Oelberg DG, Davis P, Buescher ES
JournalJ Leukoc Biol
PubMed ID9850158
'Apoptosis occurs rapidly in human polymorphonuclear leukocytes (PMN) after exposure to 1 mM cycloheximide (CHX). We examined whether this form of stimulated apoptosis altered either resting cytosolic free Ca2+ concentrations ([free Ca]) or membrane potential (psi) in PMN and found no significant effects. However, manipulation of either PMN intracellular Ca2+ ... More
Collagen I initiates endothelial cell morphogenesis by inducing actin polymerization through suppression of cyclic AMP and protein kinase A.
AuthorsWhelan MC, Senger DR
JournalJ Biol Chem
PubMed ID12399469
'Collagen I provokes endothelial cells to assume a spindle-shaped morphology and to align into solid cord-like assemblies. These cords closely imitate the solid pre-capillary cords of embryonic angiogenesis, raising interesting questions about underlying mechanisms. Studies described here identify a critical mechanism beginning with collagen I ligation of integrins alpha(1)beta(1) and ... More
Evidence for a conserved role for CRKII and Rac in engulfment of apoptotic cells.
AuthorsTosello-Trampont AC, Brugnera E, Ravichandran KS
JournalJ Biol Chem
PubMed ID11297528
'Apoptosis or programmed cell death occurs in multicellular organisms throughout life. The removal of apoptotic cells by phagocytes prevents secondary necrosis and inflammation and also plays a key role in tissue remodeling and regulating immune responses. The molecular mechanisms that regulate the engulfment of apoptotic cells are just beginning to ... More
Effects of homocysteine on proliferation, necrosis, and apoptosis of vascular smooth muscle cells in culture and influence of folic acid.
AuthorsBuemi M, Marino D, Di Pasquale G, Floccari F, Ruello A, Aloisi C, Corica F, Senatore M, Romeo A, Frisina N
JournalThromb Res
PubMed ID11672763
'BACKGROUND: It is known that hyperhomocysteinemia is associated with an increased risk of vascular disease, yet little is known about the pathogenic mechanisms underlying the action of homocysteine (Hcy) itself. METHODS: We evaluated the effects of Hcy on cell proliferation, apoptosis, and necrosis in smooth muscle cells (SMCs) cultured for ... More
Endogenous glucocorticoids induced by a chemical stressor (ethanol) cause apoptosis in the spleen in B6C3F1 female mice.
AuthorsCollier SD, Wu WJ, Pruett SB
JournalToxicol Appl Pharmacol
PubMed ID9465277
'Stress-induced increases in glucocorticoid levels can cause-apoptosis in immature thymocytes, but it is not known if glucocorticoids at these levels can also cause apoptosis in peripheral lymphocytes. In the present study, mice were exposed to ethanol (EtOH) in a model designed to represent binge drinking. This induces a substantial stress ... More
Relationship of decreased chemotaxis of technetium-99m-HMPAO-labeled lymphocytes to apoptosis.
AuthorsVan de Wiele C, Philippé J, Van Haelst JP, Van Damme J, Thierens H, Leroux-Roels GE, Dierckx RA
JournalJ Nucl Med
PubMed ID9293800
'The purpose of this study was to evaluate chemotaxis and its relationship to apoptosis in 99mTc-HMPAO-labeled lymphocytes. METHODS: Peripheral lymphocytes, obtained from 12 healthy volunteers using lymphoprep, were divided in three equal fractions. One fraction was used as the control, one was labeled with cold HMPAO and one was labeled ... More
Antiphospholipid antibodies accelerate plasma coagulation by inhibiting annexin-V binding to phospholipids: a "lupus procoagulant" phenomenon.
AuthorsRand JH, Wu XX, Andree HA, Ross JB, Rusinova E, Gascon-Lema MG, Calandri C, Harpel PC
JournalBlood
PubMed ID9716593
'The antiphospholipid syndrome is a thrombophilic condition marked by antibodies that recognize anionic phospholipid-protein cofactor complexes. We recently reported that exposure to IgG fractions from antiphospholipid patients reduces the level of annexin-V, a phospholipid-binding anticoagulant protein, on cultured trophoblasts and endothelial cells and accelerates coagulation of plasma exposed to these ... More
Bcl-2 protects against FCCP-induced apoptosis and mitochondrial membrane potential depolarization in PC12 cells.
AuthorsDispersyn G, Nuydens R, Connors R, Borgers M, Geerts H
JournalBiochim Biophys Acta
PubMed ID10434055
'This report addresses the relation between Bcl-2 and mitochondrial membrane potential (DeltaPsi(m)) in apoptotic cell death. Rat pheochromocytoma (PC12) cells are differentiated into neuron-like cells with nerve growth factor (NGF). It is known that Bcl-2 can attenuate apoptosis induced by deprivation of neurotrophic factor. The protective effect of Bcl-2 has ... More
Quantification of apoptosis in lymphocyte subsets and effect of apoptosis on apparent expression of membrane antigens.
AuthorsPhilippé J, Louagie H, Thierens H, Vral A, Cornelissen M, De Ridder L
JournalCytometry
PubMed ID9389441
'Annexin V binding to phosphatidylserine was evaluated by flow cytometry to examine apoptosis in different lymphocyte subsets of peripheral blood mononuclear cells after a 24 h in vitro culture period. We also applied a 2 Gy dose gamma-irradiation prior to incubation to evaluate the additional apoptogenic effect of radiation on ... More
Comparison of multiple assays for kinetic detection of apoptosis in thymocytes exposed to dexamethasone or diethylstilbesterol.
AuthorsDonner KJ, Becker KM, Hissong BD, Ahmed SA
JournalCytometry
PubMed ID10554184
'BACKGROUND: Techniques to measure apoptosis are used to study a wide spectrum of conditions, from acquired immune deficiency syndrome (AIDS) to cancer to autoimmune diseases. Therefore, a critical comparison of common assays for apoptosis is warranted. METHODS: The kinetics of apoptosis induction in dexamethasone (DEX)-exposed thymocytes was examined after 2, ... More
An anti-transferrin receptor-avidin fusion protein exhibits both strong proapoptotic activity and the ability to deliver various molecules into cancer cells.
AuthorsNg PP, Dela Cruz JS, Sorour DN, Stinebaugh JM, Shin SU, Shin DS, Morrison SL, Penichet ML
JournalProc Natl Acad Sci U S A
PubMed ID12149472
'We have developed an antibody fusion protein (anti-rat TfR IgG3-Av) with the ability to deliver different molecules into cancer cells. It consists of avidin genetically fused to the C(H)3 region of a human IgG3 specific for the rat transferrin receptor. It forms strong, noncovalent interactions with biotinylated molecules such as ... More
Quantification of apoptotic cells with fluorescein isothiocyanate-labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin.
AuthorsBoersma AW, Nooter K, Oostrum RG, Stoter G
JournalCytometry
PubMed ID8725661
'Plasma membrane binding of annexin V was used to detect and quantitate apoptotic cells induced by cytotoxic drug treatment in epithelial cell cultures. Chinese hamster ovary (CHO) cells were incubated for 2 h with the ID90 concentration of Cisplatin (20 microM), and 24, 48, 72, and 96 h later the ... More
Phospholipid scramblase activation pathways in lymphocytes.
AuthorsWilliamson P, Christie A, Kohlin T, Schlegel RA, Comfurius P, Harmsma M, Zwaal RF, Bevers EM
JournalBiochemistry
PubMed ID11434775
'In erythrocytes and platelets, activation of a nonspecific lipid flipsite termed the scramblase allows rapid, bidirectional transbilayer movement of all types of phospholipids. When applied to lymphoid cells, scramblase assays reveal a similar activity, with scrambling rates intermediate between those seen in platelets and erythrocytes. Scrambling activity initiated in lymphoid ... More
Early detection of apoptosis using a fluorescent conjugate of annexin V.
AuthorsZhang G, Gurtu V, Kain SR, Yan G
JournalBiotechniques
PubMed ID9298227
'Apoptosis of mammalian cells is accompanied by various morphological changes including nuclear condensation, DNA fragmentation and cell surface changes. Methods developed over the past few years have focused on detection of DNA-associated changes that occur rather late in apoptosis. However, detection of apoptosis at early stages, before gross morphological changes, ... More
Hemocompatibility of treated polystyrene substrates: contact activation, platelet adhesion, and procoagulant activity of adherent platelets.
AuthorsGrunkemeier JM, Tsai WB, Horbett TA
JournalJ Biomed Mater Res
PubMed ID9697039
'Platelet adhesion to biomaterials is often used as an index of blood compatibility, but a more clinically relevant issue is whether the adherent platelets are able to promote clot formation (i.e., if they are in the procoagulant state). Platelets rapidly generate thrombin when they are in the procoagulant state and ... More
Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis.
'Apoptosis, or programmed cell death, is a general mechanism for removal of unwanted cells from the immune system. It is characterized by chromatin condensation, a reduction in cell volume, and endonuclease cleavage of DNA into oligonucleosomal length fragments. Apoptosis is also accompanied by a loss of membrane phospholipid asymmetry, resulting ... More
Pulsed electric fields for selection of hematopoietic cells and depletion of tumor cell contaminants.
'Purging of tumor cells and selection of stem cells are key technologies for enabling stem cell transplantation and stem cell gene therapy. Here we report a strategy for cell selection based on physical properties of the cells. Exposing cells to an external pulsed electric field (PEF) increases the natural potential ... More
The K+ channel openers diazoxide and NS1619 induce depolarization of mitochondria and have differential effects on cell Ca2+ in CD34+ cell line KG-1a.
AuthorsKörper S, Nolte F, Rojewski MT, Thiel E, Schrezenmeier H
JournalExp Hematol
PubMed ID12962728
'OBJECTIVE: Mitochondrial membrane potential (deltaPsim) and intracellular Ca2+ play a crucial role in growth and differentiation in hemopoiesis. Some potassium channel openers such as diazoxide have the capacity to elevate cytosolic Ca2+ and depolarize mitochondria in cardiomyocytes. To clarify if such substances have effects on hemopoietic cells we investigated the ... More
Inhibitor of apoptosis protein (IAP) profiling in experimental autoimmune encephalomyelitis (EAE) implicates increased XIAP in T lymphocytes.
AuthorsMoore CS, Hebb AL, Robertson GS,
JournalJ Neuroimmunol
PubMed ID18055022
'In multiple sclerosis (MS) and its widely accepted animal model, experimental autoimmune encephalomyelitis (EAE), the failure of autoreactive immune cells to undergo apoptosis is thought to contribute to CNS tissue damage and disease progression. Promoting apoptosis of myelin-reactive immune cells in diseases such as MS, may delay disease progression and ... More
Surface exposure of phosphatidylserine during apoptosis of rat thymocytes precedes nuclear changes.
AuthorsStuart MC, Damoiseaux JG, Frederik PM, Arends JW, Reutelingsperger CP
JournalEur J Cell Biol
PubMed ID9650785
'Cell surface exposure of phosphatidylserine (PS) during apoptosis serves recognition and removal of the dying cell by phagocytes. Loss of phospholipid asymmetry and PS exposure is investigated by immunocytochemistry and related to morphological changes. Loss of membrane asymmetry was determined on dexamethasone-treated rat thymocytes using the PS specific probe annexin ... More
Upregulation of reactive oxygen species generation and phagocytosis, and increased apoptosis in human neutrophils during severe sepsis and septic shock.
AuthorsMartins PS, Kallas EG, Neto MC, Dalboni MA, Blecher S, Salomão R
JournalShock
PubMed ID12923490
'We evaluated neutrophil activation by measuring its phagocytic ability and oxidative burst activity in 16 patients with sepsis and 16 healthy volunteers. We also focused on neutrophil apoptosis as a regulatory mechanism of the inflammatory response. Neutrophil phagocytosis was evaluated by the detection of propidium iodide (PI)-labeled Staphylococcus aureus added ... More
Loss of phosphoserine polar group asymmetry and inhibition of cholesterol transport in Jurkat cells treated with cholesterylphosphoserine.
AuthorsCusinato F, Habeler W, Calderazzo F, Nardi F, Bruni A
JournalJ Lipid Res
PubMed ID9741697
'Cholesterylphosphoserine (CPHS) is a synthetic ester of cholesterol showing immunosuppressive activity. In the present study, we have used the T cell line Jurkat to investigate its mechanism of action. CPHS incorporates into cells reaching a molar ratio of 0.23 and 3.9 with the total phospholipid and cholesterol content, without inducing ... More
Motor deficit in a Drosophila model of mucolipidosis type IV due to defective clearance of apoptotic cells.
'Disruption of the Transient Receptor Potential (TRP) mucolipin 1 (TRPML1) channel results in the neurodegenerative disorder mucolipidosis type IV (MLIV), a lysosomal storage disease with severe motor impairments. The mechanisms underlying MLIV are poorly understood and there is no treatment. Here, we report a Drosophila MLIV model, which recapitulates the ... More
N-acetylcysteine does not protect HepG2 cells against acetaminophen-induced apoptosis.
AuthorsManov I, Hirsh M, Iancu TC
JournalBasic Clin Pharmacol Toxicol
PubMed ID15125691
'Acetaminophen in large doses is well-known as hepatotoxic, and early therapy with N-acetylcysteine is frequently life-saving. However, in later stages of acetaminophen poisoning, treatment with N-acetylcysteine is not always effective. Although some of the pathways of acetaminophen toxicity and the effect of N-acetylcysteine have been elucidated, in depth information on ... More
Cell-derived microparticles generated in patients during cardiopulmonary bypass are highly procoagulant.
AuthorsNieuwland R, Berckmans RJ, Rotteveel-Eijkman RC, Maquelin KN, Roozendaal KJ, Jansen PG, ten Have K, Eijsman L, Hack CE, Sturk A
JournalCirculation
PubMed ID9396452
'BACKGROUND: Microparticles from platelets and other cells have been extensively studied and characterized in vitro. Although the level of platelet-derived microparticles is elevated in a variety of diseases, including cardiac surgery, virtually nothing is known about their functions in vivo. The aim of the present study was to investigate the ... More
Expression, purification and use of recombinant annexin V for the detection of apoptotic cells.
AuthorsLogue SE, Elgendy M, Martin SJ,
JournalNat Protoc
PubMed ID19730422
'Apoptosis is a mode of programmed cell death that is widely used to eliminate cells during development, tissue homeostasis, infection or in response to injury. Alterations to the plasma membranes of apoptotic cells trigger recognition and engulfment of such cells by phagocytes. Measurement of plasma membrane phosphatidylserine externalization, using fluorescently ... More
Rapid annexin-V labeling in synaptosomes.
AuthorsGylys KH, Fein JA, Wiley DJ, Cole GM
JournalNeurochem Int
PubMed ID14568554
'Distal neuronal terminals may be the site of apoptotic events and early synapse loss in neurodegenerative disease. To examine apoptosis in synaptic regions, we established a cell-free assay using a rat brain crude synaptosomal preparation (P-2 fraction) as a model system. The apoptosis marker annexin-V was used to measure phosphatidylserine ... More
Surface-functionalized nanoparticle library yields probes for apoptotic cells.
AuthorsSchellenberger EA, Reynolds F, Weissleder R, Josephson L
JournalChembiochem
PubMed ID14997519
'We have developed techniques for the efficient synthesis and screening of small libraries of surface-functionalized nanoparticles for the recognition of specific types of cells. To illustrate this concept we describe the development of a nanoparticle that preferentially recognizes apoptotic Jurkat cells in a manner similar to the apoptosis-recognizing protein annexin ... More
Analysis of early apoptotic events in individual cells by fluorescence intensity and polarization measurements.
AuthorsZurgil N, Shafran Y, Fixler D, Deutsch M
JournalBiochem Biophys Res Commun
PubMed ID11820802
'Apoptosis is a dynamic process of variable duration. The ability to continuously detect the death process occurring in single or subgroups of cells is therefore very important in identifying apoptotic cells within a complex population. The Individual Cell Scanner (ICS), a multiparametric, multilaser-based scanning static cytometer, was used in the ... More
Use of the microculture kinetic assay of apoptosis to determine chemosensitivities of leukemias.
'Chemotherapeutic agents exert their antitumor effects by inducing apoptosis. The microculture kinetic (MiCK) assay provides an automated, continuous means of monitoring apoptosis in a cell population. We used the MiCK assay to determine the chemosensitivities of the human promyelocytic HL-60 and lymphoblastic CEM cell lines and leukemia cells freshly isolated ... More
Differential requirement for caspase 9 in apoptotic pathways in vivo.
AuthorsHakem R, Hakem A, Duncan GS, Henderson JT, Woo M, Soengas MS, Elia A, de la Pompa JL, Kagi D, Khoo W, Potter J, Yoshida R, Kaufman SA, Lowe SW, Penninger JM, Mak TW
JournalCell
PubMed ID9708736
'Mutation of Caspase 9 (Casp9) results in embryonic lethality and defective brain development associated with decreased apoptosis. Casp9-/- embryonic stem cells and embryonic fibroblasts are resistant to several apoptotic stimuli, including UV and gamma irradiation. Casp9-/- thymocytes are also resistant to dexamethasone- and gamma irradiation-induced apoptosis, but are surprisingly sensitive ... More
Imaging of apoptosis (programmed cell death) with 99mTc annexin V.
AuthorsBlankenberg FG, Katsikis PD, Tait JF, Davis RE, Naumovski L, Ohtsuki K, Kopiwoda S, Abrams MJ, Strauss HW
JournalJ Nucl Med
PubMed ID9935075
'Apoptosis (programmed cell death) is a critical element in normal physiology and in many disease processes. Phosphatidylserine (PS), one component of cell membrane phospholipids, is normally confined to the inner leaflet of the plasma membrane. Early in the course of apoptosis, this phospholipid is rapidly exposed on the cell's outer ... More
Changes in endoplasmic reticulum luminal environment affect cell sensitivity to apoptosis.
AuthorsNakamura K, Bossy-Wetzel E, Burns K, Fadel MP, Lozyk M, Goping IS, Opas M, Bleackley RC, Green DR, Michalak M
JournalJ Cell Biol
PubMed ID10952999
'To test the role of ER luminal environment in apoptosis, we generated HeLa cell lines inducible with respect to calreticulin and calnexin and investigated their sensitivity to drug-dependent apoptosis. Overexpression of calreticulin, an ER luminal protein, resulted in an increased sensitivity of the cells to both thapsigargin- and staurosporine-induced apoptosis. ... More
Organization and dynamics of the proteolipid complexes formed by annexin V and lipids in planar supported lipid bilayers.
AuthorsCézanne L, Lopez A, Loste F, Parnaud G, Saurel O, Demange P, Tocanne JF
JournalBiochemistry
PubMed ID10052949
'The consequences of the binding of annexin V on its lateral mobility and that of lipids were investigated by means of experimental and simulated FRAP experiments. Experiments were carried out on planar supported bilayers (PC/PS 9:1 mol/mol mixtures) in the presence of 1 mM CaCl2 in the subphase. The probes ... More
Annexin V staining due to loss of membrane asymmetry can be reversible and precede commitment to apoptotic death.
AuthorsHammill AK, Uhr JW, Scheuermann RH
JournalExp Cell Res
PubMed ID10438567
'Signal-induced apoptosis is a normal phenomenon in which cells respond to changes in their environment through a cascade of intracellular biochemical changes culminating in cell death. However, it is not clear at what point in this process the cell becomes committed to die. An early biochemical change characteristic of cells ... More
An oxidative stress-mediated death pathway in irradiated human leukemia cells mapped using multilaser flow cytometry.
AuthorsSheng-Tanner X, Bump EA, Hedley DW
JournalRadiat Res
PubMed ID9840183
'OCI/AML-2 acute myeloid leukemia cells were found to undergo apoptosis after treatment with y rays from a 137Cs source. Multilaser flow cytometry techniques using probes for live cell function were used to monitor the biochemical changes that occurred prior to the loss of surface membrane integrity. These showed increases in ... More
Caspase dependence of target cell damage induced by cytotoxic lymphocytes.
AuthorsSarin A, Haddad EK, Henkart PA
JournalJ Immunol
PubMed ID9743340
'Since the CTL secreted granule protease granzyme B can activate multiple target caspases, it has been proposed that this pathway is responsible for CTL-induced cytolysis of Fas-negative targets. However, target lysis via the granule exocytosis pathway is completely resistant to caspase inhibitors. To test the possibility that granzymes trigger a ... More
Melatonin prevents death of neuroblastoma cells exposed to the Alzheimer amyloid peptide.
AuthorsPappolla MA, Sos M, Omar RA, Bick RJ, Hickson-Bick DL, Reiter RJ, Efthimiopoulos S, Robakis NK
JournalJ Neurosci
PubMed ID9030627
'Studies from several laboratories have generated evidence suggesting that oxidative stress is involved in the pathogenesis of Alzheimer''s disease (AD). The finding that the amyloid beta protein (Abeta) has neurotoxic properties and that such effects are, in part, mediated by free radicals has provided insights into mechanisms of cell death ... More
Comparison of annexin V and calcein-AM as early vital markers of apoptosis in adherent cells by confocal laser microscopy.
'Although morphological criteria for apoptosis are in general reliable, no systematic comparison of the techniques employed thus far has yet been performed. In this study, using confocal laser microscopy, we compared the performance of annexin V-FITC and calcein-AM for early detection of apoptosis in living adherent cells. Experiments were carried ... More
FKHR-L1 can act as a critical effector of cell death induced by cytokine withdrawal: protein kinase B-enhanced cell survival through maintenance of mitochondrial integrity.
AuthorsDijkers PF, Birkenkamp KU, Lam EW, Thomas NS, Lammers JW, Koenderman L, Coffer PJ
JournalJ Cell Biol
PubMed ID11815629
'Survival signals elicited by cytokines include the activation of phosphatidylinositol 3-kinase (PI3K), which in turn promotes the activation of protein kinase B (PKB). Recently, PKB has been demonstrated to phosphorylate and inactivate forkhead transcription factor FKHR-L1, a potent inducer of apoptosis. To explore the mechanisms underlying the induction of apoptosis ... More
Prolongation of human neutrophil survival by low-level mercury via inhibition of spontaneous apoptosis.
AuthorsMoisan E, Arbour S, Nguyen N, Hébert MJ, Girard D, Bernier J, Fournier M, Kouassi E
JournalJ Toxicol Environ Health A
PubMed ID11820505
'Low levels of organic and inorganic mercury compounds have been reported previously to induce cell death by apoptosis in human peripheral blood mononuclear cells (MNC). but little is known about their potential effects on the viability and death of polymorphonuclear neutrophils (PMN). In contrast to MNC, PMN are known to ... More
Indocyanine green induces apoptosis in human retinal pigment epithelial cells.
AuthorsRezai KA, Farrokh-Siar L, Ernest JT, van Seventer GA
JournalAm J Ophthalmol
PubMed ID15126160
'PURPOSE: To examine whether indocyanine green (ICG) dye induces apoptosis in human retinal pigment epithelial (RPE) cells. DESIGN: Laboratory investigation. METHODS: Pure cultures of human RPE cells were isolated. Retinal pigment epithelial cells were incubated with different concentrations of ICG dye (1 mg/ml, 5 mg/ml, or 20 mg/ml) for 30 ... More
Synergistic increase in cell lethality by dieldrin and H2O2 in rat thymocytes: Effect of dieldrin on the cells exposed to oxidative stress.
AuthorsChimeddorj T, Suzuki T, Murakane K, Inai M, Satoh M, Oyama Y,
Journal
PubMed ID23726008
'Dieldrin, one of persistent pesticides, is highly resistant to biotic and abiotic degradation. It is accumulated in organisms. Recent studies suggest that dieldrin exerts a potent cytotoxic action on cells exposed to oxidative stress. In this study, the effect of dieldrin on rat thymocytes exposed to hydrogen peroxide (H2O2)-induced oxidative ... More
Comparative measurement of spontaneous apoptosis in pediatric acute leukemia by different techniques.
AuthorsSavitskiy VP, Shman TV, Potapnev MP
JournalCytometry B Clin Cytom
PubMed ID14582133
'BACKGROUND: To distinguish between subgroups of patients with acute leukemia, the rate of spontaneous (culture-induced) apoptosis of leukemic cells was evaluated using five methods. METHODS: Leukemic cells (cells) from the bone marrow of children with acute lymphoblastic leukemia (ALL, n = 112) and acute myeloid leukemia (AML, n = 30) ... More
Carbon nanotubes as intracellular protein transporters: generality and biological functionality.
AuthorsKam NW, Dai H
JournalJ Am Chem Soc
PubMed ID15839702
'Various proteins adsorb spontaneously on the sidewalls of acid-oxidized single-walled carbon nanotubes. This simple nonspecific binding scheme can be used to afford noncovalent protein-nanotube conjugates. The proteins are found to be readily transported inside various mammalian cells with nanotubes acting as the transporter via the endocytosis pathway. Once released from ... More
Latrunculin B or ATP depletion induces cofilin-dependent translocation of actin into nuclei of mast cells.
AuthorsPendleton A, Pope B, Weeds A, Koffer A
JournalJ Biol Chem
PubMed ID12566455
'Increasing cellular G-actin, using latrunculin B, in either intact or permeabilized rat peritoneal mast cells, caused translocation of both actin and an actin regulatory protein, cofilin, into the nuclei. The effect was not associated with an increase in the proportion of apoptotic cells. The major part of the nuclear actin ... More
Differential protective activity of alpha A- and alphaB-crystallin in lens epithelial cells.
AuthorsAndley UP, Song Z, Wawrousek EF, Fleming TP, Bassnett S
JournalJ Biol Chem
PubMed ID10967101
'alphaA- and alphaB-crystallins are molecular chaperones expressed at low levels in lens epithelial cells, and their expression increases dramatically during differentiation to lens fibers. However, the functions of alphaA- and alphaB-crystallins in lens epithelial cells have not been studied in detail. In this study, the relative ability of alphaA- and ... More
Akt-dependent antiapoptotic action of insulin is sensitive to farnesyltransferase inhibitor.
AuthorsPark D, Pandey SK, Maksimova E, Kole S, Bernier M
JournalBiochemistry
PubMed ID11027130
'CHO cells expressing the human insulin receptors (IR) were used to evaluate the effect of the potent farnesyltransferase inhibitor, manumycin, on insulin antiapoptotic function. Cell treatment with manumycin blocked insulin''s ability to suppress pro-apoptotic caspase-3 activity which led to time-dependent proteolytic cleavage of two nuclear target proteins. The Raf-1/MEK/ERK cascade ... More
A decrease in intracellular zinc level precedes the detection of early indicators of apoptosis in HL-60 cells.
AuthorsDuffy JY, Miller CM, Rutschilling GL, Ridder GM, Clegg MS, Keen CL, Daston GP
JournalApoptosis
PubMed ID11388665
'Low extracellular zinc concentrations have been associated with the induction of apoptosis. To assess the relationship between intracellular zinc concentration and the rate of apoptosis, cells were grown in media containing 0.5, 25, or 50 microM zinc and analyzed by flow cytometry or fluorescence microscopy. Cells grown in 0.5 microM ... More
Delta9-tetrahydrocannabinol induces apoptosis in human prostate PC-3 cells via a receptor-independent mechanism.
AuthorsRuiz L, Miguel A, Díaz-Laviada I
JournalFEBS Lett
PubMed ID10570948
'The effect of delta9-tetrahydrocannabinol (THC), the major psycho-active component of marijuana, in human prostate cancer cells PC-3 was investigated. THC caused apoptosis in a dose-dependent manner. Morphological and biochemical changes induced by THC in prostate PC-3 cells shared the characteristics of an apoptotic phenomenon. First, loss of plasma membrane asymmetry ... More
Targeting neural-restrictive silencer factor sensitizes tumor cells to antibody-based cancer immunotherapy in vitro via multiple mechanisms.
AuthorsKolev MV, Ruseva MM, Morgan BP, Donev RM,
JournalJ Immunol
PubMed ID20421646
'Tumor cells escape clearance by complement by abundantly expressing CD59 and other membrane complement regulators. Recently, we designed a peptide derived from the neural-restrictive silencer factor (REST), REST68, which we showed to inhibit expression of CD59 in tumors lacking the full-length REST and proposed a detailed model for regulation of ... More
Differential involvement of MEK kinase 1 (MEKK1) in the induction of apoptosis in response to microtubule-targeted drugs versus DNA damaging agents.
AuthorsGibson S, Widmann C, Johnson GL
JournalJ Biol Chem
PubMed ID10196170
'MEK kinase 1 (MEKK1) is a 196-kDa enzyme that is involved in the regulation of the c-Jun N-terminal kinase (JNK) pathway and apoptosis. In cells exposed to genotoxic agents including etoposide and cytosine arabinoside, MEKK1 is cleaved at Asp874 by caspases. The cleaved kinase domain of MEKK1, itself, stimulates caspase ... More