Regulation of the Fas death pathway by FLICE-inhibitory protein in primary human B cells.
AuthorsHennino A, Berard M, Casamayor-Pallejà M, Krammer PH, Defrance T
JournalJ Immunol
PubMed ID10975811
'The Fas/Fas ligand (L) system plays an important role in the maintenance of peripheral B cell tolerance and the prevention of misguided T cell help. CD40-derived signals are required to induce Fas expression on virgin B cells and to promote their susceptibility to Fas-mediated apoptosis. In the current study, we ... More
Detection of apoptotic cells by annexin V labeling at electron microscopy.
AuthorsPellicciari C, Bottone MG, Biggiogera M
JournalEur J Histochem
PubMed ID9359032
'Annexin V is a Ca(++)-binding protein which is widely used as a marker for apoptotic cells, as it binds to the phosphatidylserine residues exposed at the surface of apoptotic cells. In this paper, we describe a method for the immunogold-labeling of biotin-conjugated Annexin V, to detect apoptotic thymocytes at electron ... More
Spatiotemporal distribution of dying neurons during early mouse development.
Authorsvan den Eijnde SM, Lips J, Boshart L, Vermeij-Keers C, Marani E, Reutelingsperger CP, De Zeeuw CI
JournalEur J Neurosci
PubMed ID10051772
'Apoptosis is a critical cellular event during several stages of neuronal development. Recently, we have shown that biotinylated annexin V detects apoptosis in vivo in various cell lineages of a wide range of species by binding to phosphatidylserines that are exposed at the outer leaflet of the plasma membrane. In ... More
In situ detection of apoptosis during embryogenesis with annexin V: from whole mount to ultrastructure.
Authorsvan den Eijnde SM, Luijsterburg AJ, Boshart L, De Zeeuw CI, van Dierendonck JH, Reutelingsperger CP, Vermeij-Keers C
JournalCytometry
PubMed ID9415414
'Apoptosis is of paramount importance during embryonic development. This insight stems from early studies which correlated cell death to normal developmental processes and now has been confirmed by linking aberrant cell death patterns to aberrant development. Linking apoptosis to the phenotype of a developing organism requires spatial information on the ... More
Complement-independent, peroxide-induced antibody lysis of platelets in HIV-1-related immune thrombocytopenia.
AuthorsNardi M, Tomlinson S, Greco MA, Karpatkin S
JournalCell
PubMed ID11551503
'Immunologic thrombocytopenia is seen commonly in HIV-1 infection. The pathogenesis of this problem has been unclear, but it is associated with circulating immune complexes that contain platelet membrane components and anti-platelet membrane GPIIIa49-66 IgG antibodies. These antibodies cause acute thrombocytopenia when injected into mice. We now show that purified anti-GPIIIa49-66 ... More
A novel assay to measure loss of plasma membrane asymmetry during apoptosis of adherent cells in culture.
Authorsvan Engeland M, Ramaekers FC, Schutte B, Reutelingsperger CP
JournalCytometry
PubMed ID8725662
'Early during the process of apoptosis, cells lose their phospholipid membrane asymmetry and expose phosphatidylserine (PS) at the cell surface while maintaining their plasma membrane integrity intact. This process can be monitored for suspended cell types by using annexin V-FITC, which is a Ca(2+)-dependent, phospholipid-binding protein with high affinity for ... More
Using the Microcyte flow cytometer to monitor cell number, viability, and apoptosis in mammalian cell culture.
AuthorsHarding CL, Lloyd DR, McFarlane CM, Al-Rubeai M
JournalBiotechnol Prog
PubMed ID11027173
The Microcyte is a novel, portable flow cytometer based on diode laser technology whose use has been established for yeast and bacterial analysis. We present data that demonstrate its suitability for routine mammalian cell counting and viability determination. To extend its range of applications in the field of animal cell ... More
Transcriptional profiling of antigen-dependent murine B cell differentiation and memory formation.
AuthorsBhattacharya D, Cheah MT, Franco CB, Hosen N, Pin CL, Sha WC, Weissman IL,
JournalJ Immunol
PubMed ID17982071
Humoral immunity is characterized by the generation of Ab-secreting plasma cells and memory B cells that can more rapidly generate specific Abs upon Ag exposure than their naive counterparts. To determine the intrinsic differences that distinguish naive and memory B cells and to identify pathways that allow germinal center B ... More
Oral nickel tolerance: Fas ligand-expressing invariant NK T cells promote tolerance induction by eliciting apoptotic death of antigen-carrying, effete B cells.
AuthorsNowak M, Kopp F, Roelofs-Haarhuis K, Wu X, Gleichmann E,
JournalJ Immunol
PubMed ID16585548
Whereas oral nickel administration to C57BL/6 mice (Ni(high) mice) renders the animals tolerant to immunization with NiCl2 combined with H2O2 as adjuvant, as determined by ear-swelling assay, it fails to tolerize Jalpha18-/- mice, which lack invariant NKT (iNKT) cells. Our previous work also showed that Ni(high) splenic B cells can ... More
Quantum dots based probes conjugated to annexin V for photostable apoptosis detection and imaging.
AuthorsLe Gac S, Vermes I, van den Berg A
JournalNano Lett
PubMed ID16967992
Quantum dots (Qdots) are nanoparticles exhibiting fluorescent properties that can be used for cell staining. We present here the development of quantum dots conjugated to Annexin V for specific targeting of apoptotic cells, for both apoptosis detection and staining of apoptotic "living" cells. For that purpose, Qdots Streptavidin Conjugates are ... More
NF-kappaB inhibits apoptosis in murine mammary epithelia.
AuthorsClarkson RW, Heeley JL, Chapman R, Aillet F, Hay RT, Wyllie A, Watson CJ
JournalJ Biol Chem
PubMed ID10777569
The transcription factor NF-kappaB is a key modulator of apoptosis in a variety of cell types, but to date this specific function of NF-kappaB has not been demonstrated in epithelia. Here, we describe the activation of NF-kappaB during post-lactational involution of the mouse mammary gland, a period of extensive apoptosis ... More
Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure.
Apoptosis is a programmed, physiological mode of cell death that plays an important role in tissue homeostasis. Understanding of the basic mechanisms that underlie apoptosis will point to potentially new targets of therapeutic treatment of diseases that show an imbalance between cell proliferation and cell loss. In order to conduct ... More
A novel IkappaB protein, IkappaB-zeta, induced by proinflammatory stimuli, negatively regulates nuclear factor-kappaB in the nuclei.
AuthorsYamazaki S, Muta T, Takeshige K
JournalJ Biol Chem
PubMed ID11356851
The transcription factor nuclear factor-kappaB (NF-kappaB) plays crucial roles in a wide variety of cellular functions and its activity is strictly regulated by cytosolic inhibitors known as IkappaBs. We here report a new member of the IkappaB protein family, IkappaB-zeta, harboring six ankyrin repeats at its carboxyl terminus. IkappaB-zeta mRNA ... More
Annexin V binds to positively selected B cells.
AuthorsDillon SR, Constantinescu A, Schlissel MS
JournalJ Immunol
PubMed ID11123277
Recombinant annexin V (rAnV) has been used in flow cytometry to identify cells undergoing apoptosis, based on its ability to bind to phosphatidylserine, a negatively charged lipid normally restricted to the cytoplasmic face of the plasma membrane but externalized early during apoptosis. When we stained murine bone marrow (BM) cells ... More