Electroosmotic flow control of fluids on a capillary electrophoresis microdevice using an applied external voltage
AuthorsPolson NA, Hayes MA
JournalAnal Chem
PubMed ID10739217
'Independent control of electroosmosis is important for separation science techniques such as capillary zone electrophoresis and for the movement of fluids on microdevices. A capillary electrophoresis microdevice is demonstrated which provides more efficient control of electroosmosis with an applied external voltage field. The device is fabricated in a glass substrate ... More
Excited-state dynamics of the fluorescent probe Lucifer Yellow in liquid solutions and in heterogeneous media.
AuthorsFürstenberg A, Vauthey E
JournalPhotochem Photobiol Sci
PubMed ID15738993
The photophysics of the dye Lucifer Yellow ethylenediamine (LYen) has been investigated in various polar solvents. The main deactivation pathways of its first singlet excited state are the fluorescence and the intersystem crossing. In water, non-radiative decay by intermolecular proton transfer becomes a significant deactivation channel. The early fluorescence dynamics, ... More
Site-dependent excited-state dynamics of a fluorescent probe bound to avidin and streptavidin.
AuthorsFürstenberg A, Kel O, Gradinaru J, Ward TR, Emery D, Bollot G, Mareda J, Vauthey E,
JournalChemphyschem
PubMed ID19565577
The excited-state dynamics of biotin-spacer-Lucifer-Yellow (LY) constructs bound to avidin (Avi) and streptavidin (Sav) was investigated using femtosecond spectroscopy. Two different locations in the proteins, identified by molecular dynamics simulations of Sav, namely the entrance of the binding pocket and the protein surface, were probed by varying the length of ... More
Cryopreservation of collagen-based tissue equivalents. I. Effect of freezing in the absence of cryoprotective agents.
The effect of freezing on the viability and mechanical properties of tissue-equivalents (TEs) was determined under a variety of cooling conditions, with the ultimate aim of optimizing the cryopreservation process. TEs (a class of bioartificial tissues) were prepared by incubating entrapped human foreskin fibroblasts in collagen gels for a period ... More