Effects of intracellular angiotensin II in vascular smooth muscle cells.
AuthorsHaller H, Lindschau C, Erdmann B, Quass P, Luft FC
JournalCirc Res
PubMed ID8831500
'Angiotensin (Ang) II is present inside vascular smooth muscle cells (VSMCs); however, its intracellular functions, if any, are unknown. We tested the hypothesis that intracellular Ang II exerts effects on cytosolic Ca2+ ([Ca2+]i) in VSMCs. Ang II was administered via microinjection. Intracellular Ang II localization was demonstrated by fluorescein-labeled Ang ... More
Intracellular third loop domain of angiotensin II type-2 receptor. Role in mediating signal transduction and cellular function.
Authors Hayashida W; Horiuchi M; Dzau V J;
JournalJ Biol Chem
PubMed ID8703004
'The present study tests the hypothesis that the unique intracellular third loop domain of angiotensin II type-2 (AT2) receptor is essential for the subsequent intracellular signaling and plays an important role in mediating receptor function. Synthetic intracellular third loop peptide of the AT2 receptor (AT2-3LP, 22 amino acids) and control ... More
AT1 receptor-mediated accumulation of extracellular angiotensin II in proximal tubule cells: role of cytoskeleton microtubules and tyrosine phosphatases.
AuthorsLi XC, Carretero OA, Navar LG, Zhuo JL,
JournalAm J Physiol Renal Physiol
PubMed ID16478976
Long-term angiotensin II (ANG II) administration is associated with increased ANG II accumulation in the kidney, but intrarenal compartment(s) involved in this response remains to be determined. We tested the hypothesis that 1) extracellular ANG II is taken up by proximal tubule cells (PTCs) through AT(1) receptor-mediated endocytosis, 2) this ... More
Intracellular trafficking of angiotensin II and its AT1 and AT2 receptors: evidence for selective sorting of receptor and ligand.
AuthorsHein L, Meinel L, Pratt RE, Dzau VJ, Kobilka BK
JournalMol Endocrinol
PubMed ID9259318
Angiotensin II (Ang II) binds to two different receptor subtypes, AT1 and AT2 receptors. In many cases, receptor stimulation by Ang II is followed by a rapid desensitization of the intracellular signal transduction and a decrease in cell surface receptor number. The present study was designed to examine by immunofluorescence ... More
Angiotensins stimulate catecholamine release from the chromaffin tissue of the rainbow trout.
AuthorsBernier NJ, Perry SF
JournalAm J Physiol
PubMed ID9249532
Immunohistochemical and pharmacological techniques were utilized to investigate the relationships between angiotensins and catecholamine release from the chromaffin tissue of rainbow trout (Oncorhynchus mykiss). Double labeling with [Asp1, Ile5]angiotensin II-fluorescein isothiocyanate (ANG II-FITC) and anti-dopamine beta-hydroxylase revealed specific ANG II binding sites on chromaffin cells. Injection (1 nmol/kg body wt) ... More