Redesigning an FKBP-ligand interface to generate chemical dimerizers with novel specificity.
AuthorsClackson T, Yang W, Rozamus LW, Hatada M, Amara JF, Rollins CT, Stevenson LF, Magari SR, Wood SA, Courage NL, Lu X, Cerasoli F, Gilman M, Holt DA
JournalProc Natl Acad Sci U S A
PubMed ID9724721
'FKBP ligand homodimers can be used to activate signaling events inside cells and animals that have been engineered to express fusions between appropriate signaling domains and FKBP. However, use of these dimerizers in vivo is potentially limited by ligand binding to endogenous FKBP. We have designed ligands that bind specifically ... More
4'-[Aminomethyl]fluorescein and its N-alkyl derivatives: useful reagents in immunodiagnostic techniques.
AuthorsShipchandler MT, Fino JR, Klein LD, Kirkemo CL
JournalAnal Biochem
PubMed ID3605599
'A new class of fluorescein derivatives with chemically reactive amino and N-alkylamino "arms" in the 4''-position were synthesized and their utility in the development of fluorescence polarization immunoassays (FPIA) for cortisol and estriol was evaluated. The positioning of the arm in one of the phenolic rings introduced chirality due to ... More
Swelling-based method for preparing stable, functionalized polymer colloids.