Fluorescence polarization: analysis of carbohydrate-protein interaction.
AuthorsKakehi K, Oda Y, Kinoshita M
JournalAnal Biochem
PubMed ID11673876
'Fluorescence polarization has been widely used for the studies on the molecular motion in solution and has been applied to immunoassays for proteins, therapeutic drug monitoring in clinical pharmacy, and assays for environmentally toxic compounds. Because fluorescence polarization is most readily applicable to the kinetic analysis of the binding reaction ... More
Synthesis of a novel fluorescent probe for estrogen receptor.
AuthorsAdamczyk M, Reddy RE, Yu Z
JournalBioorg Med Chem Lett
PubMed ID11965371
A novel estradiol-mimetic fluorescent probe 5 was synthesized from diethylstilbestrol (DES, 1), which is useful for probing estrogen receptor (ERalpha), a prognostic indicator of estrogen-dependent cancers, and for developing a homogeneous fluorescence polarization (FP) assay to identify the ligands of estrogen receptor. ... More
Immunoassay reagents for thyroid testing. 1. Synthesis of thyroxine conjugates.
AuthorsAdamczyk M, Fino L, Fishpaugh JR, Johnson DD, Mattingly PG
JournalBioconjug Chem
PubMed ID7849077
Immunoreagents were designed to improve the performance of a commercial fluorescent polarization immunoassay for thyroxine. The thyroxine immunogen was prepared by selective coupling of N-acetyl-L-thyroxine to BSA via an aminocaproic acid spacer arm. The fluorescent tracer was prepared by a multistep reaction sequence which relied on extensive use of orthogonal ... More
A novel FRET approach for in situ investigation of cellulase-cellulose interaction.
AuthorsWang L, Wang Y, Ragauskas AJ,
JournalAnal Bioanal Chem
PubMed ID20694721
A novel real-time in situ detection method for the investigation of cellulase-cellulose interactions based on fluorescence resonance energy transfer (FRET) has been developed. FRET has been widely used in biological and biophysical fields for studies related to proteins, nucleic acids, and small biological molecules. Here, we report the efficient labeling ... More
Buffering properties of cationic polymethacrylates are not the only key to successful gene delivery.
AuthorsDubruel P, Christiaens B, Rosseneu M, Vandekerckhove J, Grooten J, Goossens V, Schacht E
JournalBiomacromolecules
PubMed ID15002997
Recently, we have shown that polymethacrylates containing imidazole side groups (HYMIMMA) or acid functions (MA), which have similar buffering properties as polyethyleneimine, were not able to transfect Cos-1 cells, whereas polymers containing only tertiary amines (DMAEMA) do transfect Cos-1 cells (Dubruel, P. et al. Eur. J. Pharm. Sci. 2003, 18 ... More
Crocus sativus lectin recognizes Man3GlcNAc in the N-glycan core structure.
AuthorsOda Y, Nakayama K, Abdul-Rahman B, Kinoshita M, Hashimoto O, Kawasaki N, Hayakawa T, Kakehi K, Tomiya N, Lee YC
JournalJ Biol Chem
PubMed ID10837483
Crocus sativus lectin (CSL) is one of the truly mannose-specific plant lectins that has a unique binding specificity that sets it apart from others. We studied sugar-binding specificity of CSL in detail by a solution phase method (fluorescence polarization) and three solid phase methods (flow injection, surface plasmon resonance, and ... More