Localization of acidic residues involved in the proton pumping activity of the bovine heart mitochondrial bc1 complex.
AuthorsCocco T, Di Paola M, Papa S, Lorusso M
JournalFEBS Lett
PubMed ID10452525
'Chemical modification of carboxyl residues in polypeptide subunits of the mitochondrial bc1 complex causes a decoupling effect, that is inhibition of the proton pumping activity, without affecting the rate of electron transfer to ferricytochrome c. The study presented here is aimed at localizing and identifying the residues whose modification results ... More
4'-[Aminomethyl]fluorescein and its N-alkyl derivatives: useful reagents in immunodiagnostic techniques.
AuthorsShipchandler MT, Fino JR, Klein LD, Kirkemo CL
JournalAnal Biochem
PubMed ID3605599
'A new class of fluorescein derivatives with chemically reactive amino and N-alkylamino "arms" in the 4''-position were synthesized and their utility in the development of fluorescence polarization immunoassays (FPIA) for cortisol and estriol was evaluated. The positioning of the arm in one of the phenolic rings introduced chirality due to ... More
Major histocompatibility complex independent T cell receptor-antigen interaction: functional analysis using fluorescein derivatives.
We have isolated T cell receptor (TCR) cDNAs from fluorescein (FL)-specific human T cell clones (alpha FL beta FL), and transferred them to TCR beta- Jurkat cells in order to study direct FL-binding to the TCR. Using either FL-conjugated polymers (FL-polymer) or FL-substituted Sepharose beads, we are able to demonstrate ... More
Laser modification of preformed polymer microchannels: application to reduce band broadening around turns subject to electrokinetic flow.
AuthorsJohnson TJ, Ross D, Gaitan M, Locascio LE
JournalAnal Chem
PubMed ID11510830
A pulsed UV excimer laser (KrF, 248 nm) was used to modify the surface charge on the side wall of hot-embossed microchannels fabricated in a poly(methyl methacrylate) substrate. Subablation level fluences, less than 2,385 mJ/cm2, were used to prevent any changes in the physical morphology of the surface. It is ... More
Carboxyl residues in the iron-sulfur protein are involved in the proton pumping activity of P. denitrificans bc(1) complex.
AuthorsCocco T, Cutecchia G, Ludwig B, Korn M, Papa S, Lorusso M
JournalBiochemistry
PubMed ID11735423
A study is presented on chemical modification of the three subunit Paracoccus denitrificans bc(1) complex. N-(Ethoxycarbonyl)-2-ethoxy-1,2-dihydroquinoline (EEDQ) treatment caused a loss of the proton pumping activity of liposome-reconstituted bc(1) complex. A similar effect, which is referred to as the decoupling effect, resulted upon reaction of N,N'-dicyclohexylcarbodiimide (DCCD) with the complex. ... More