CTS™ KnockOut™ DMEM/F-12

Gibco™

CTS™ KnockOut™ DMEM/F-12

CTS KnockOut DMEM/F-12 is a serum free, low osmolality medium without L-glutamine or HEPES buffer that is optimized for the growth of human embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC).

Catalog Number	Quantity
A1370801	500 mL

Catalog number A1370801

Price (CNY)

1,053.00

Add to cart

Quantity:

500 mL

Price (CNY)

1,053.00

Add to cart

Gibco CTS KnockOut DMEM/F-12 is a serum free, low osmolality medium without L-glutamine or HEPES buffer that is optimized for the growth of human embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC). It is designed for use with KnockOut SR and KnockOut SR XenoFree CTS supplements.

Consistent results without fetal bovine serum

Designed for consistent performance, CTS KnockOut DMEM/F-12 is part of the KnockOut line of media products that have delivered reliable results for pluripotent stem cell (ESC and iPSC) culture for years. This fetal bovine free medium leads to little or no spontaneous differentiation.

Equivalent morphology

Stem cells cultured in CTS KnockOut DMEM/F-12 media show normal morphology and karyotype when compared to control stem cells. You can also expect equivalent morphology to manually passaged hESCs, even after enzymatic passaging.

Simplified transition into clinical research

The CTS product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications. Gibco CTS products are of high quality and are supplied with harmonized documentation such as Certificates of Analysis, Certificates of Origin, and access to authorization letters for our Drug Master Files (DMF).

For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals.

Specifications

Cell TypeEmbryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)

For Use With (Application)Cell and Gene Therapy Research, Development, and Manufacturing

FormatBottle

Manufacturing QualityISO 13485, MDSAP, FDA-registered, 21 CFR 820

Product TypeCell Culture Medium

Quantity500 mL

Shelf Life18 months from date of manufacture

Shipping ConditionAmbient

ClassificationAnimal Origin-free

FormLiquid

SterilitySterile-filtered

Unit SizeEach

Contents & Storage

Store at 2–8°C and protect from light.

Frequently asked questions (FAQs)

What is CTS?

The Gibco Cell Therapy Systems (CTS) portfolio of cell and gene therapy products are GMP manufactured, safety tested, and backed by regulatory documentation to support your transition from discovery through clinical and commercial manufacturing. Through our CTS solutions, we are committed to helping customers streamline therapeutic development, minimize risk, and ease the burden on their quality systems. Learn more here.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer DMEM/F12 medium without folic acid?

We do not offer DMEM/F12 medium without folic acid as a standard catalog product. However, our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an “official” order or declining.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all CTS™ KnockOut™ DMEM/F-12 FAQs