Serum-free suspension culturing of human cells: adaptation, growth, and cryopreservation.
AuthorsBiaggio RT, Abreu-Neto MS, Covas DT, Swiech K
JournalBioprocess Biosyst Eng
PubMed ID25822314
Human cell lines have attracted great interest because they are capable of producing glycosylated proteins that are more similar to native human proteins, thereby reducing the potential for immune responses. However, these cells have not been extensively characterized and cultured under serum-free suspension conditions. In this work, we describe the ... More
Amnion epithelial cell isolation and characterization for clinical use.
AuthorsMurphy S, Rosli S, Acharya R, Mathias L, Lim R, Wallace E, Jenkin G
JournalCurr Protoc Stem Cell Biol
PubMed ID20373516
Human amnion epithelial cells (hAECs) are a heterologous population positive for stem cell markers; they display multilineage differentiation potential, differentiating into cells of the endoderm (liver, lung epithelium), mesoderm (bone, fat), and ectoderm (neural cells). They have a low immunogenic profile and possess potent immunosuppressive properties. Hence, hAECs may be ... More
Cellular pharmacodynamics of the novel biaryloxazolidinone radezolid: studies with infected phagocytic and nonphagocytic cells, using Staphylococcus aureus, Staphylococcus epidermidis, Listeria monocytogenes, and Legionella pneumophila.
AuthorsLemaire S, Kosowska-Shick K, Appelbaum PC, Verween G, Tulkens PM, Van Bambeke F
JournalAntimicrob Agents Chemother
PubMed ID20385852
Radezolid is a novel biaryloxazolidinone in clinical development which shows improved activity, including against linezolid-resistant strains. In a companion paper (29), we showed that radezolid accumulates about 11-fold in phagocytic cells, with approximately 60% of the drug localized in the cytosol and approximately 40% in the lysosomes of the cells. ... More
Rapid and Efficient Generation of Transgene-Free iPSC from a Small Volume of Cryopreserved Blood.
AuthorsZhou H, Martinez H, Sun B, Li A, Zimmer M, Katsanis N, Davis EE, Kurtzberg J, Lipnick S, Noggle S, Rao M, Chang S
Journal
PubMed ID25951995
Human peripheral blood and umbilical cord blood represent attractive sources of cells for reprogramming to induced pluripotent stem cells (iPSCs). However, to date, most of the blood-derived iPSCs were generated using either integrating methods or starting from T-lymphocytes that have genomic rearrangements thus bearing uncertain consequences when using iPSC-derived lineages ... More
Biological impact of xeno-free chemically defined cryopreservation medium on amniotic epithelial cells.
AuthorsMiki T, Wong W, Zhou E, Gonzalez A, Garcia I, Grubbs BH
JournalStem Cell Res Ther
PubMed ID26758986
'Amnion-derived stem cells have been proposed for cell replacement therapy and tissue regeneration. An easily accessible cell source, the placenta, allows us to potentially establish a bio-bank of cells for immunotype matched clinical applications. Several xeno-free (XF) cryopreservation media are currently available for pluripotent stem cells, however, these media have ... More
Technological developments for small-scale downstream processing of cell therapies.
Despite considerable regulatory and clinical hurdles, the development and use of cell-based therapies are gaining momentum. As more of these therapies move toward commercial approval and larger-scale distribution, associated manufacturing and processing technologies are being advanced. Modern technologies directed at downstream processing seek to distribute such therapies from the manufacturing ... More