Mitochondria dysfunction is considered to be a major cause of the modifications that occur during cell ageing. For this reason, cardiolipin, a suitable marker of the chondriome, as well as the mitochondrial transmembrane potential were examined in keratinocytes obtained from 9- to 75-year-old women. The study was carried out by ... More
Tamoxifen but not 4-hydroxytamoxifen initiates apoptosis in p53(-) normal human mammary epithelial cells by inducing mitochondrial depolarization.
AuthorsDietze EC, Caldwell LE, Grupin SL, Mancini M, Seewaldt VL
JournalJ Biol Chem
PubMed ID11094056
'Despite the widespread clinical use of tamoxifen as a breast cancer prevention agent, the molecular mechanism of tamoxifen chemoprevention is poorly understood. Abnormal expression of p53 is felt to be an early event in mammary carcinogenesis. We developed an in vitro model of early breast cancer prevention to investigate how ... More
Sortase A localizes to distinct foci on the Streptococcus pyogenes membrane.
AuthorsRaz A, Fischetti VA,
JournalProc Natl Acad Sci U S A
PubMed ID19017791
'Cell wall peptidoglycan-anchored surface proteins are essential virulence factors in many gram-positive bacteria. The attachment of these proteins to the peptidoglycan is achieved through a transpeptidation reaction, whereby sortase cleaves a conserved C-terminal LPXTG motif and covalently attaches the protein to the peptidoglycan precursor lipid II. It is unclear how ... More
Methods for assessing autophagy and autophagic cell death.
AuthorsTasdemir E, Galluzzi L, Maiuri MC, Criollo A, Vitale I, Hangen E, Modjtahedi N, Kroemer G,
JournalMethods Mol Biol
PubMed ID18425442
'Autophagic (or type 2) cell death is characterized by the massive accumulation of autophagic vacuoles (autophagosomes) in the cytoplasm of cells that lack signs of apoptosis (type 1 cell death). Here we detail and critically assess a series of methods to promote and inhibit autophagy via pharmacological and genetic manipulations. ... More
Bcl-xL regulates the membrane potential and volume homeostasis of mitochondria.
'Mitochondrial physiology is disrupted in either apoptosis or necrosis. Here, we report that a wide variety of apoptotic and necrotic stimuli induce progressive mitochondrial swelling and outer mitochondrial membrane rupture. Discontinuity of the outer mitochondrial membrane results in cytochrome c redistribution from the intermembrane space to the cytosol followed by ... More
Rhodamine-123 staining in hematopoietic stem cells of young mice indicates mitochondrial activation rather than dye efflux.
AuthorsKim M, Cooper DD, Hayes SF, Spangrude GJ
JournalBlood
PubMed ID9596656
'Low-intensity fluorescence of rhodamine-123 (Rh-123) discriminates a quiescent hematopoietic stem cell (HSC) population in mouse bone marrow, which provides stable, long-term hematopoiesis after transplantation. Rh-123 labels mitochondria with increasing intensity proportional to cellular activation, however the intensity of staining also correlates with the multidrug resistance (MDR) phenotype, as Rh-123 is ... More
Vitamin E deficiency impairs the modifications of mitochondrial membrane potential and mass in rat splenocytes stimulated to proliferate.
AuthorsPieri C, Moroni F, Recchioni R
JournalFree Radic Biol Med
PubMed ID7511124
'This study was designed to evaluate the time-dependent changes of mitochondrial membrane potential and mass during Con-A-induced proliferation of splenic lymphocytes from rat fed a normal or a vitamin E deficient diet. Rhodamine 123 and Nonyl Acridine Orange were used as specific probes to monitor the membrane potential and mass ... More
Nitric oxide-dependent mitochondrial biogenesis generates Ca2+ signaling profile of lupus T cells.
AuthorsNagy G, Barcza M, Gonchoroff N, Phillips PE, Perl A
JournalJ Immunol
PubMed ID15356113
'Abnormal T cell activation and cell death underlie the pathology of systemic lupus erythematosus. Although mitochondrial hyperpolarization (MHP) represents an early and reversible checkpoint of T cell activation and apoptosis, lupus T cells exhibit persistent MHP. NO has recently been recognized as a key signal of mitochondrial biogenesis and mediator ... More
Use of nonyl acridine orange and rhodamine 123 to follow biosynthesis and functional assembly of mitochondrial membrane during L1210 cell cycle.
AuthorsLeprat P, Ratinaud MH, Maftah A, Petit JM, Julien R
JournalExp Cell Res
PubMed ID1688800
'Specific mitochondrial incorporation of 10 N-nonyl acridine orange (NAO) is demonstrated by subcellular fractionation of rat hepatocytes. Moreover, comparative studies with NAO and rhodamine 123 (Rh 123) prove that acridine orange-derivative uptake is independent of transmembrane mitochondrial potential, a property allowing its utilization for the assessment of mitochondrial membrane mass ... More
N-t-Butyl hydroxylamine is an antioxidant that reverses age-related changes in mitochondria in vivo and in vitro.
'N-t-butyl hydroxylamine (NtBHA) delays senescence-dependent changes in human lung fibroblasts (IMR90) (Atamna et al., J. Biol. Chem. 275, 6741-6748). The current study examines the effect of NtBHA on mitochondria in old and young rats and human primary fibroblasts (IMR90). In NtBHA-treated rats, the age-dependent decline in food consumption and ambulatory ... More
Rhodamine 123: a useful probe for monitoring T cell activation.
AuthorsFerlini C, Biselli R, Nisini R, Fattorossi A
JournalCytometry
PubMed ID8582251
'The T cell activation pathway involves an increase in mitochondrial activity. This can be evaluated in individual cells using the fluorescent probe rhodamine 123 (Rh123) and flow cytometry. Peripheral blood mononuclear cells (PBMC) were stimulated with optimal concentrations of phytohaemagglutinin (PHA), superantigens (Sag) SEA and SEC2, and allogeneic cells. Activation ... More
Detection of changes in mitochondrial function during apoptosis by simultaneous staining with multiple fluorescent dyes and correlated multiparameter flow cytometry.
AuthorsPoot M, Pierce RH
JournalCytometry
PubMed ID10213196
'BACKGROUND: The possible relationships between changes in mitochondrial membrane potential and other mitochondrial functions during apoptosis remain controversial. METHODS: To detect concomitant changes in mitochondrial function during apoptosis, we performed correlated multiparameter flow cytometry after simultaneous cell staining with several dyes. RESULTS: After camptothecin treatment, nonapoptotic cells exhibited a concomitant ... More
Changes of mitochondrial mass in the hemopoietic stem cell line FDCP-mix after treatment with etoposide: a correlative study by multiparameter flow cytometry and confocal and electron microscopy.
AuthorsReipert S, Berry J, Hughes MF, Hickman JA, Allen TD
JournalExp Cell Res
PubMed ID7493625
'FDCP-Mix, a pluripotent murine hemopoietic stem cell line which undergoes typical internucleosomal cleavage of DNA when induced to apoptosis by either drugs or withdrawal of growth factor (interleukin-3) was studied after treatment with the topoisomerase II inhibitor etoposide (0.5-4 microM). An increase in autolytic activity was the major early morphological ... More
Comparison of the effect of mitochondrial inhibitors on mitochondrial membrane potential in two different cell lines using flow cytometry and spectrofluorometry.
AuthorsKalbácová M, Vrbacký M, Drahota Z, Melková Z
JournalCytometry A
PubMed ID12655654
'BACKGROUND: Determination of mitochondrial membrane potential (DeltaPsim) is widely used to characterize cellular metabolism, viability, and apoptosis. Changes of DeltaPsim induced by inhibitors of oxidative phosphorylation characterize respective contributions of mitochondria and glycolysis to adenosine triphosphate (ATP) synthesis. METHODS: DeltaPsim in BSC-40 and HeLa G cell lines was determined by ... More
Poly(ADP-ribose) synthetase activation mediates mitochondrial injury during oxidant-induced cell death.
AuthorsVirág L, Salzman AL, Szabó C
JournalJ Immunol
PubMed ID9759901
'Reactive oxidant species are important mediators of tissue injury in shock, inflammation, and reperfusion injury. The actions of a number of these oxidants (e.g., hydroxyl radical and peroxynitrite, a reactive oxidant produced by the reaction of nitric oxide and superoxide) are mediated in part by the activation of the nuclear ... More
[Hydrophobic acridine dyes for fluorescence staining of mitochondria in living cells. 1.Thermodynamic and spectroscopic properties of 10-n-alkylacridine orange chlorides]
AuthorsSeptinus M, Seiffert W, Zimmermann HW
JournalHistochemistry
PubMed ID6197394
'10-n-Alkyl-acridine-orange-chlorides (alkyl-AOs) are excellent dyes for fluorescence staining of mitochondria in living cells. The thermodynamic and spectroscopic properties of the series alkyl = methyl to nonyl have been investigated. The dyes form dimers in aqueous solution. The dimerisation is mainly a consequence of the hydrophobic interaction. The dissociation constant K ... More
The impairment of mitochondrial membrane potential and mass in proliferating lymphocytes from vitamin E deficient animals is recovered by glutathione.
AuthorsPieri C, Recchioni R, Marcheselli F, Moroni F, Marra M, Benatti C
JournalCell Mol Biol (Noisy-le-grand)
PubMed ID8535168
'The time-dependent changes of mitochondrial membrane potential and mass have been investigated on splenocytes from control and vit. E deficient rats, stimulated to proliferate with Concanavalin A, in the presence and absence of reduced glutathione (GSH, 5 mM). Rhodamine-123 (Rh-123) and nonyl acridine orange (NAO) were used as specific probes ... More
Mitochondrial mass and membrane potential in coelomocytes from the earthworm Eisenia foetida: studies with fluorescent probes in single intact cells.
AuthorsCossarizza A, Cooper EL, Quaglino D, Salvioli S, Kalachnikova G, Franceschi C
JournalBiochem Biophys Res Commun
PubMed ID7677758
'Earthworm coelomocytes exist in two forms, i.e., small (SC) and large (LC) cells, as demonstrated by velocity sedimentation, electron microscopy, and FCM. However, we know little concerning the functional activities of various, important organelles, such as mitochondria. In comparison with SC, LC from Eisenia foetida have a higher number of ... More
Hydrogen peroxide overproduction in megamitochondria of troglitazone-treated human hepatocytes.
AuthorsShishido S, Koga H, Harada M, Kumemura H, Hanada S, Taniguchi E, Kumashiro R, Ohira H, Sato Y, Namba M, Ueno T, Sata M
JournalHepatology
PubMed ID12500198
'Troglitazone has been withdrawn from therapeutic options for diabetes mellitus because of its severe hepatocyte toxicity of unknown pathogenesis. The aim of the present study was to assess both morphologic and functional alterations in the mitochondria of troglitazone-treated hepatocytes. A polarized human hepatocyte cell line, OUMS-29, was used in this ... More
10N-nonyl acridine orange interacts with cardiolipin and allows the quantification of this phospholipid in isolated mitochondria.
AuthorsPetit JM, Maftah A, Ratinaud MH, Julien R
JournalEur J Biochem
PubMed ID1396703
'The acridine orange derivative, 10N-nonyl acridine orange, is an appropriate marker of the inner mitochondrial membrane in whole cells. We use membrane model systems to demonstrate that 10N-nonyl acridine orange binds to negatively charged phospholipids (cardiolipin, phosphatidylinositol and phosphatidylserine). The stoichiometry has been found to be 2 mol 10N-nonyl acridine ... More
Compared flow cytometric analysis of mitochondria using 10-n-nonyl acridine orange and rhodamine 123.
AuthorsBenel L, Ronot X, Mounolou JC, Gaudemer F, Adolphe M
JournalBasic Appl Histochem
PubMed ID2757602
'The use of the supravital mitochondrial-specific dye Rhodamine 123 (Rh 123) in combination with flow cytometry permits the monitoring of the changes in the mitochondrial transmembrane potential, reflecting the overall mitochondrial activity of the living cell. While this probe appears to be a potent tool for these studies, it also ... More
Persistent oxidative stress in chromosomally unstable cells.
AuthorsLimoli CL, Giedzinski E, Morgan WF, Swarts SG, Jones GD, Hyun W
JournalCancer Res
PubMed ID12810636
'Past work using the human-hamster hybrid line GM10115 has demonstrated that exposure to a variety of DNA damaging agents can lead to the persistent destabilization of chromosomes. To gain insight into the potential biochemical mechanisms involved in perpetuating the unstable phenotype, groups of clones characterized as stable or unstable were ... More
Mitochondria play no roles in Mn(II)-induced apoptosis in HeLa cells.
AuthorsOubrahim H, Stadtman ER, Chock PB
JournalProc Natl Acad Sci U S A
PubMed ID11493712
'Manganese(II) has been shown to exhibit catalase-like activity under physiological conditions. In the course of studies to test the antioxidant activity of Mn(II) on HeLa cells, it was observed at high concentrations (1-2 mM) that Mn(II) also induced apoptosis, as judged by changes in cell morphology, caspase-3 activation, cleavage of ... More
Sequence of metabolic changes during X-ray-induced apoptosis.
AuthorsFerlini C, De Angelis C, Biselli R, Distefano M, Scambia G, Fattorossi A
JournalExp Cell Res
PubMed ID10047458
'A cell population undergoing apoptosis usually contains varying proportions of cells in the diverse stages of the process, from very early continuously through to secondary necrosis. This heterogeneity acts as a confounding factor in metabolic studies if a general population is investigated. Using fluorescent probes and multiparameter flow cytometry, we ... More
Visualization of phospholipid domains in Escherichia coli by using the cardiolipin-specific fluorescent dye 10-N-nonyl acridine orange.
AuthorsMileykovskaya E, Dowhan W
JournalJ Bacteriol
PubMed ID10648548
'Cardiolipin (CL)-specific fluorescent dye 10-N-nonyl-acridine orange (NAO) was used to visualize CL distribution in Escherichia coli cells of different phospholipid compositions. In a filamentous mutant containing only anionic phospholipids, green fluorescent spots were observed along the filaments at approximately regular intervals. Three-dimensional image reconstruction obtained by optical sectioning and a ... More
Binding of 10-N-nonyl acridine orange to cardiolipin-deficient yeast cells: implications for assay of cardiolipin.
AuthorsGohil VM, Gvozdenovic-Jeremic J, Schlame M, Greenberg ML
JournalAnal Biochem
PubMed ID15963941
Probing mitochondrial probes.
AuthorsIsola R, Falchi AM, Diana A, Diaz G
JournalCytometry
PubMed ID11002271
Behavior of mitochondria in the living cell.
AuthorsBereiter-Hahn J
JournalInt Rev Cytol
PubMed ID2246114
Assessment of mitochondrial membrane potential in situ using single potentiometric dyes and a novel fluorescence resonance energy transfer technique.
AuthorsDykens JA, Stout AK
JournalMethods Cell Biol
PubMed ID11381600
10-N nonyl-acridine orange: a fluorescent probe which stains mitochondria independently of their energetic state.
AuthorsMaftah A, Petit JM, Ratinaud MH, Julien R
JournalBiochem Biophys Res Commun
PubMed ID2478126
The specificity of binding of 10-N Nonyl Acridine Orange to mitochondria, and more precisely to inner membranes, is demonstrated by subcellular fractionation of hepatocytes. Unlike Rhodamine 123, which is a preferential marker of the transmembrane potential, Nonyl Acridine Orange binding is essentially independent of the mitochondria energization state although a ... More
Mitochondrial autophagy is an HIF-1-dependent adaptive metabolic response to hypoxia.
AuthorsZhang H, Bosch-Marce M, Shimoda LA, Tan YS, Baek JH, Wesley JB, Gonzalez FJ, Semenza GL,
JournalJ Biol Chem
PubMed ID18281291
Autophagy is a process by which cytoplasmic organelles can be catabolized either to remove defective structures or as a means of providing macromolecules for energy generation under conditions of nutrient starvation. In this study we demonstrate that mitochondrial autophagy is induced by hypoxia, that this process requires the hypoxia-dependent factor-1-dependent ... More
Mitochondria are selective targets for the protective effects of heat shock against oxidative injury.
AuthorsPolla BS, Kantengwa S, François D, Salvioli S, Franceschi C, Marsac C, Cossarizza A
JournalProc Natl Acad Sci U S A
PubMed ID8692837
Heat shock (HS) proteins (HSPs) induce protection against a number of stresses distinct from HS, including reactive oxygen species. In the human premonocytic line U937, we investigated in whole cells the effects of preexposure to HS and exposure to hydrogen peroxide (H2O2) on mitochondrial membrane potential, mass, and ultrastructure. HS ... More
Changes in intramitochondrial cardiolipin distribution in apoptosis-resistant HCW-2 cells, derived from the human promyelocytic leukemia HL-60.
AuthorsGarcia Fernandez M, Troiano L, Moretti L, Pedrazzi J, Salvioli S, Castilla-Cortazar I, Cossarizza A
JournalFEBS Lett
PubMed ID10930584
Using a cytofluorimetric approach, we studied intramitochondrial cardiolipin (CL) distribution in HCW-2 cells, an apoptosis-resistant clone of human HL-60 cells. In HL-60, about 50% of total CL is distributed in the outer leaflet of mitochondrial inner membrane, while in HCW-2 a significantly higher amount of CL (about 65%) is in ... More
In situ flow cytometric analysis of nonyl acridine orange-stained mitochondria from splenocytes.
AuthorsRatinaud MH, Leprat P, Julien R
JournalCytometry
PubMed ID2454177
Due to its spectral characteristics, the fluorochrome nonyl acridine orange (NAO) (lambda abs:489 nm, lambda em:525 nm), which is spontaneously incorporated by mitochondria with a high relative specificity, provides a new probe for the in situ study of these organelles by flow cytometry. In 15 min at 20 degrees C, ... More
Cardiolipin binds nonyl acridine orange by aggregating the dye at exposed hydrophobic domains on bilayer surfaces.
10-N-Nonyl acridine orange (NAO) has been used at low concentrations as a fluorescent indicator for cardiolipin (CL) in membranes and bilayers. The mechanism of its selective fluorescence in the presence of CL, and not any other phospholipids, is not understood. The dye might recognize CL by its high pK (pK(2)>8.5). ... More
[Hydrophobic acridine dyes for fluorescence staining of mitochondria in living cells. 2. Comparison of staining of living and fixed Hela-cells with NAO and DPPAO]
AuthorsErbrich U, Septinus M, Naujok A, Zimmermann HW
JournalHistochemistry
PubMed ID6547420
The hydrophobic fluorescence dyes NAO and DPPAO (see scheme of structural formulae) stain the mitochondria of living HeLa-cells. The trans-membrane potential favours the dye accumulation of the cation NAO and supports the hydrophobic interaction of the dye with the mitochondrial membrane lipids and proteins. The lecithin-like dye DPPAO is electrical ... More
Uptake of fluorescent dyes associated with the functional expression of the cystic fibrosis transmembrane conductance regulator in epithelial cells.
AuthorsWersto RP, Rosenthal ER, Crystal RG, Spring KR
JournalProc Natl Acad Sci U S A
PubMed ID8577734
Specific mutations in the cystic fibrosis transmembrane conductance regulator (CFTR), the most common autosomal recessive fatal genetic disease of Caucasians, result in the loss of epithelial cell adenosine 3',5'-cyclic-monophosphate (cAMP)-stimulated Cl- conductance. We show that the influx of a fluorescent dye, dihydrorhodamine 6G (dR6G), is increased in cells expressing human ... More
Downregulation of multiple stress defense mechanisms during differentiation of human embryonic stem cells.
AuthorsSaretzki G, Walter T, Atkinson S, Passos JF, Bareth B, Keith WN, Stewart R, Hoare S, Stojkovic M, Armstrong L, von Zglinicki T, Lako M,
JournalStem Cells
PubMed ID18055443
Evolutionary theory predicts that cellular maintenance, stress defense, and DNA repair mechanisms should be most active in germ line cells, including embryonic stem cells that can differentiate into germ line cells, whereas it would be energetically unfavorable to keep these up in mortal somatic cells. We tested this hypothesis by ... More
Loss of autophagy in erythroid cells leads to defective removal of mitochondria and severe anemia in vivo.
AuthorsMortensen M, Ferguson DJ, Edelmann M, Kessler B, Morten KJ, Komatsu M, Simon AK,
JournalProc Natl Acad Sci U S A
PubMed ID20080761
Timely elimination of damaged mitochondria is essential to protect cells from the potential harm of disordered mitochondrial metabolism and release of proapoptotic proteins. In mammalian red blood cells, the expulsion of the nucleus followed by the removal of other organelles, such as mitochondria, are necessary differentiation steps. Mitochondrial sequestration by ... More
Transaldolase deficiency influences the pentose phosphate pathway, mitochondrial homoeostasis and apoptosis signal processing.
AuthorsQian Y, Banerjee S, Grossman CE, Amidon W, Nagy G, Barcza M, Niland B, Karp DR, Middleton FA, Banki K, Perl A,
JournalBiochem J
PubMed ID18498245
TAL (transaldolase) was originally described in the yeast as an enzyme of the PPP (pentose phosphate pathway). However, certain organisms and mammalian tissues lack TAL, and the overall reason for its existence is unclear. Recently, deletion of Ser(171) (TALDeltaS171) was found in five patients causing inactivation, proteasome-mediated degradation and complete ... More
Nitric oxide mediates T cell cytokine production and signal transduction in histidine decarboxylase knockout mice.
AuthorsKoncz A, Pasztoi M, Mazan M, Fazakas F, Buzas E, Falus A, Nagy G,
JournalJ Immunol
PubMed ID17982051
Histamine is a key regulator of the immune system. Several lines of evidence suggest the role of histamine in T cell activation and accelerated Th1 immune response is a hallmark of histidine decarboxylase knockout (HDC-KO) mice, with a complete lack of endogenously produced histamine. According to our previous work, T ... More
Bcl-xL prevents cell death following growth factor withdrawal by facilitating mitochondrial ATP/ADP exchange.
Growth factor withdrawal is associated with a metabolic arrest that can result in apoptosis. Cell death is preceded by loss of outer mitochondrial membrane integrity and cytochrome c release. These mitochondrial events appear to follow a relative increase in mitochondrial membrane potential. This change in membrane potential results from the ... More
Age-dependent modifications of mitochondrial trans-membrane potential and mass in rat splenic lymphocytes during proliferation.
AuthorsPieri C, Recchioni R, Moroni F
JournalMech Ageing Dev
PubMed ID8246634
The specific fluorescent probes, Rhodamine 123 (Rh-123) and Nonyl-Acridine Orange (NAO) were, respectively, used to monitor the changes in membrane potential and mass of lymphocyte mitochondria during aging and proliferation. An age-dependent increase of the uptake of both fluorochromes was observed in resting cells; however, NAO fluorescence increased to a ... More
On the mechanism underlying calcein-induced cytotoxicity.
AuthorsLiminga G, Jonsson B, Nygren P, Larsson R
JournalEur J Pharmacol
PubMed ID10594326
The cellular pharmacology of calcein acetoxymethyl ester (calcein/AM)-induced cytotoxicity was investigated in human tumor cell lines in order to identify tentative mechanisms of action. The activity profile in 10 cell lines with known mechanisms of resistance was compared with the activity profiles of standard drugs and experimental substances. The activity ... More
The Saccharomyces cerevisiae MFS superfamily SGE1 gene confers resistance to cationic dyes.
AuthorsJacquot C, Julien R, Guilloton M
JournalYeast
PubMed ID9271105
A gene from Saccharomyces cerevisiae whose overexpression confers resistance to 10-N-nonyl acridine orange (NAO) has been isolated. This cationic dye binds acidic phospholipids and more specifically cardiolipin (Petit, J. M., Maftah, A., Ratinaud, M. H. and Julien, R. Eur. J. Biochem. 209, 267-273, 1992). The isolated gene was found to ... More
Determination of electrophoretic mobility distributions through the analysis of individual mitochondrial events by capillary electrophoresis with laser-induced fluorescence detection.
AuthorsDuffy CF, Fuller KM, Malvey MW, O'Kennedy R, Arriaga EA
JournalAnal Chem
PubMed ID11795787
Here we report on the analysis of mitochondrial preparations by capillary electrophoresis with postcolumn laser-induced fluorescence detection. Individual mitochondria are detected by fluorescent labeling with the mitochondrion-selective probe, 10-nonyl acridine orange. Interactions between the organelles and the capillary walls are controlled by derivatization of the capillaries with poly(acryloylaminopropanol). As expected ... More
Quantitation of mitochondrial alterations associated with apoptosis.
AuthorsCastedo M, Ferri K, Roumier T, Métivier D, Zamzami N, Kroemer G
JournalJ Immunol Methods
PubMed ID12072177
Mitochondria undergo two major changes during early apoptosis. On the one hand, the outer mitochondrial membrane becomes permeable to proteins, resulting in the release of soluble intermembrane proteins (SIMPs) from the mitochondrion. On the other hand, the inner mitochondrial membrane transmembrane potential (DeltaPsi(m)) is reduced. These changes occur in most, ... More
Three-parameter flow cytometric analysis of rat spermatogenesis.
AuthorsSuter L, Koch E, Bechter R, Bobadilla M
JournalCytometry
PubMed ID9012383
Mammalian spermatogenesis is a complex process which is not yet fully understood. In this paper we describe the analysis of rat spermatogenesis by means of 3-parameter flow cytometry. Since the analysis of DNA content only provides sufficient information for the identification of 4 cell populations, additional parameters were combined with ... More
Cytofluorometric analysis of chondrotoxicity of fluoroquinolone antimicrobial agents.
AuthorsHayem G, Petit PX, Levacher M, Gaudin C, Kahn MF, Pocidalo JJ
JournalAntimicrob Agents Chemother
PubMed ID8192451
To better understand quinolone-related arthropathy, we conceived an experimental ex vivo model using cell cultures of articular chondrocytes issued from pretreated New Zealand White rabbits (NZW). Juvenile (4- to 5-week-old) NZW were orally dosed with ofloxacin or pefloxacin (300 mg/kg of body weight for 1 day) or with pefloxacin (300 ... More
Creation and characterization of mitochondrial DNA-depleted cell lines with "neuronal-like" properties.
AuthorsMiller SW, Trimmer PA, Parker WD, Davis RE
JournalJ Neurochem
PubMed ID8863494
Mitochondrial dysfunction and attendant bioenergetic defects are increasingly recognized as playing an important role in neurodegenerative disorders. The increased attention on mitochondrial involvement points to the need for developing cell lines that have neuron-like characteristics for the genetic analysis and modeling of these diseases. We describe the creation of respiratory-deficient ... More
Mitochondrial free radical signal in ceramide-dependent apoptosis: a putative mechanism for neuronal death in Parkinson's disease.
AuthorsFrance-Lanord V, Brugg B, Michel PP, Agid Y, Ruberg M
JournalJ Neurochem
PubMed ID9326290
Activation of the apoptogenic sphingomyelin-dependent signaling pathway in neuronally differentiated PC12 cells with cell-permeant C2-ceramide resulted in a transient and short-lived emission of reactive oxygen species that was maximal 6 h after the beginning of treatment, followed immediately by nuclear translocation of the transcription factor nuclear factor kappaB. The production ... More
Reactive oxygen species derived from the mitochondrial respiratory chain are not responsible for the basal levels of oxidative base modifications observed in nuclear DNA of Mammalian cells.
The mitochondrial electron transport chain (ETC) is the most important source of reactive oxygen species (ROS) in mammalian cells. To assess its relevance to the endogenous generation of oxidative DNA damage in the nucleus, we have compared the background (steady-state) levels of oxidative DNA base modifications sensitive to the repair ... More
Mitochondria and caspases in induced apoptosis in human luteinized granulosa cells.
AuthorsKhan SM, Dauffenbach LM, Yeh J
JournalBiochem Biophys Res Commun
PubMed ID10708590
Apoptosis occurs as a physiologic process in the ovarian life cycle. Staurosporine, a protein kinase inhibitor, is reported to induce apoptosis. Here, we hypothesize that staurosporine will induce apoptosis in human luteinized granulosa cells and that mitochondria and the caspase cascade participate in this process. Luteinized granulosa cells isolated from ... More
Mitochondrial modifications during rat thymocyte apoptosis: a study at the single cell level.
AuthorsCossarizza A, Kalashnikova G, Grassilli E, Chiappelli F, Salvioli S, Capri M, Barbieri D, Troiano L, Monti D, Franceschi C
JournalExp Cell Res
PubMed ID8082735
Apoptosis is an active type of cell death, occurring under several physiological and pathological conditions. The role of cellular organelles such as mitochondria in this process is still an open question. We recently described a new method to measure mitochondrial membrane potential in intact cells using flow cytometry. Using this ... More
Mouse testis cell sorting according to DNA and mitochondrial changes during spermatogenesis.
AuthorsPetit JM, Ratinaud MH, Cordelli E, Spanò M, Julien R
JournalCytometry
PubMed ID7796695
Flow cytometry can measure variations in DNA content and chromatin structure as well as dramatic changes in the mitochondria of germ cells during maturation from spermatogonia to elongated spermatids. Using 10-N nonyl acridine orange (NAO), an inner mitochondrial membrane dye, it is easy to follow mitochondria rearrangements. Mouse testis cells ... More
The use and effects of vital fluorescent dyes: observation of motor nerve terminals and satellite cells in living frog muscles.
AuthorsHerrera AA, Banner LR
JournalJ Neurocytol
PubMed ID2351996
Several different fluorescent mitochondrial dyes were tested as vital stains for motor nerve terminals and other cells in frog skeletal muscles. It was found that 3,3' diethyloxadicarbocyanine iodide and 4-(4-diethylaminostyryl)-N-methylpyridinium iodide were most useful. Both dyes labelled motor nerve terminals with high reliability. Electrophysiological and morphological control experiments showed that ... More
A new method for testing cell ageing using two mitochondria specific fluorescent probes.
AuthorsLeprat P, Ratinaud MH, Julien R
JournalMech Ageing Dev
PubMed ID2325431
Cell culture techniques have considerably improved our understanding of the numerous changes related to aging. For instance, murine lymphocytes obtained from animals older than 6 months progressively lose their, in vitro, proliferative capacity. Numerous studies have shown that this loss is due to changes in the mitochondrial compartment such as ... More
Isolation and characterization of the gene (CLS1) encoding cardiolipin synthase in Saccharomyces cerevisiae.
AuthorsChang SC, Heacock PN, Mileykovskaya E, Voelker DR, Dowhan W
JournalJ Biol Chem
PubMed ID9614098
In eukaryotic cells, cardiolipin (CL) synthase catalyzes the final step in the synthesis of CL from phosphatidylglycerol and CDP-diacylglycerol. CL and its synthesis are localized predominantly to the mitochondrial inner membrane, and CL is generally thought to be an essential component of many mitochondrial processes. By using homology searches for ... More
Use of the fluorescent dye 10-N-nonyl acridine orange in quantitative and location assays of cardiolipin: a study on different experimental models.
AuthorsGarcia Fernandez MI, Ceccarelli D, Muscatello U
JournalAnal Biochem
PubMed ID15113694
The fluorescent dye 10-N-nonyl acridine orange (NAO) is extensively used for location and quantitative assays of cardiolipin in living cells on the assumption of its high specificity for cardiolipin; however, the limits and the mechanism of this specificity are not clear. Moreover, whether factors such as the membrane potential in ... More
A model for p53-induced apoptosis.
AuthorsPolyak K, Xia Y, Zweier JL, Kinzler KW, Vogelstein B
JournalNature
PubMed ID9305847
The inactivation of the p53 gene in a large proportion of human cancers has inspired an intense search for the encoded protein's physiological and biological properties. Expression of p53 induces either a stable growth arrest or programmed cell death (apoptosis). In human colorectal cancers, the growth arrest is dependent on ... More
Bid-cardiolipin interaction at mitochondrial contact site contributes to mitochondrial cristae reorganization and cytochrome C release.
AuthorsKim TH, Zhao Y, Ding WX, Shin JN, He X, Seo YW, Chen J, Rabinowich H, Amoscato AA, Yin XM
JournalMol Biol Cell
PubMed ID15107464
Release of cytochrome c from the mitochondrial intermembrane space is critical to apoptosis induced by a variety of death stimuli. Bid is a BH3-only prodeath Bcl-2 family protein that can potently activate this efflux. In the current study, we investigated the mitochondrial localization of Bid and its interactions with mitochondrial ... More
Glutathione is a factor of resistance of Jurkat leukemia cells to nitric oxide-mediated apoptosis.
AuthorsUmansky V, Rocha M, Breitkreutz R, Hehner S, Bucur M, Erbe N, Dröge W, Ushmorov A
JournalJ Cell Biochem
PubMed ID10861855
We have previously reported that nitric oxide (NO) stimulates apoptosis in different human neoplastic lymphoid cell lines through mitochondrial damage (including degradation of cardiolipin, a major mitochondrial lipid) followed by activation of caspases. Here we demonstrate that Jurkat human leukemia cells which survive after 24 h treatment with NO form ... More
Direct analysis and significance of cardiolipin transverse distribution in mitochondrial inner membranes.
AuthorsPetit JM, Huet O, Gallet PF, Maftah A, Ratinaud MH, Julien R
JournalEur J Biochem
PubMed ID8143741
The distribution of cardiolipin across the inner mitochondrial membrane was directly determined by using the ability of the fluorescent dye 10-N-nonyl-3,6-bis(dimethylamino)acridine (10-N-nonyl acridine orange) to form dimers when it interacts with the diacidic phospholipid. Two independent methods were employed: (a) a spectrophotometric measurement of 10-N-nonyl acridine orange binding to isolated ... More
Intracellular distribution of the fluorescent dye nonyl acridine orange responds to the mitochondrial membrane potential: implications for assays of cardiolipin and mitochondrial mass.
AuthorsJacobson J, Duchen MR, Heales SJ
JournalJ Neurochem
PubMed ID12124423
Cardiolipin, a polyunsaturated acidic phospholipid, is found exclusively in bacterial and mitochondrial membranes where it is intimately associated with the enzyme complexes of the respiratory chain. Cardiolipin structure and concentration are central to the function of these enzyme complexes and damage to the phospholipid may have consequences for mitochondrial function. ... More
Alterations in mitochondrial structure and function are early events of dexamethasone-induced thymocyte apoptosis.
AuthorsPetit PX, Lecoeur H, Zorn E, Dauguet C, Mignotte B, Gougeon ML
JournalJ Cell Biol
PubMed ID7790370
In this paper we used a multiparametric approach to analyze extensively the events occurring during apoptotic cell death of thymocytes, and furthermore, we asked whether alterations in mitochondrial structure and function are occurring in early stages of apoptosis. A multiparametric quantitative analysis was performed on normal or apoptotic thymocytes emerging ... More
SERCA1 truncated proteins unable to pump calcium reduce the endoplasmic reticulum calcium concentration and induce apoptosis.
AuthorsChami M, Gozuacik D, Lagorce D, Brini M, Falson P, Peaucellier G, Pinton P, Lecoeur H, Gougeon ML, le Maire M, Rizzuto R, Bréchot C, Paterlini-Bréchot P
JournalJ Cell Biol
PubMed ID11402072
By pumping calcium from the cytosol to the ER, sarco/endoplasmic reticulum calcium ATPases (SERCAs) play a major role in the control of calcium signaling. We describe two SERCA1 splice variants (S1Ts) characterized by exon 4 and/or exon 11 splicing, encoding COOH terminally truncated proteins, having only one of the seven ... More
Functional F1-ATPase essential in maintaining growth and membrane potential of human mitochondrial DNA-depleted rho degrees cells.
AuthorsBuchet K, Godinot C
JournalJ Biol Chem
PubMed ID9722521
F1-ATPase assembly has been studied in human rho degrees cells devoid of mitochondrial DNA (mtDNA). Since, in these cells, oxidative phosphorylation cannot provide ATP, their growth relies on glycolysis. Despite the absence of the mtDNA-coded F0 subunits 6 and 8, rho degrees cells possessed normal levels of F1-ATPase alpha and ... More
Flow cytometric analysis of human epidermal cell ageing using two fluorescent mitochondrial probes.
AuthorsDumas M, Maftah A, Bonte F, Ratinaud MH, Meybeck A, Julien R
JournalC R Acad Sci III
PubMed ID7757813
Cardiolipin, mitochondrial transmembrane potential, cell refringence and cell diameter were examined in epidermal cells obtained from 42 women between 9- to 75-year-old. The study was carried out in situ by flow cytometry on cells having incorporated either Nonyl Acridine Orange or Rhodamine 123, 2 mitochondria-specific dyes. Cardiolipin levels, determined by ... More
The biochemistry of programmed cell death.
AuthorsKroemer G, Petit P, Zamzami N, Vayssière JL, Mignotte B
JournalFASEB J
PubMed ID7557017
Programmed cell death (PCD) is involved in the removal of superfluous and damaged cells in most organ systems. The induction phase of PCD or apoptosis is characterized by an extreme heterogeneity of potential PCD-triggering signal transduction pathways. During the subsequent effector phase, the numerous PCD-inducing stimuli converge into a few ... More
The role of a mitochondrial pathway in the induction of apoptosis by chemicals extracted from diesel exhaust particles.
AuthorsHiura TS, Li N, Kaplan R, Horwitz M, Seagrave JC, Nel AE
JournalJ Immunol
PubMed ID10946301
We are interested in the cytotoxic and proinflammatory effects of particulate pollutants in the respiratory tract. We demonstrate that methanol extracts made from diesel exhaust particles (DEP) induce apoptosis and reactive oxygen species (ROS) in pulmonary alveolar macrophages and RAW 264.7 cells. The toxicity of these organic extracts mimics the ... More
Recording of mitochondrial transmembrane potential and volume in cultured rat osteoclasts by confocal laser scanning microscopy.
Osteoclasts are multinuclear bone-resorbing cells which contain abundant mitochondria. Morphological studies have suggested that a correlation may exist between mitochondrial concentration and bone resorption by osteoclasts. However, investigation of mitochondrial transmembrane potential (delta psi) and volume has been hampered by the difficulty in obtaining a sufficient number of osteoclasts for ... More
Mitochondrial phospholipid hydroperoxide glutathione peroxidase inhibits the release of cytochrome c from mitochondria by suppressing the peroxidation of cardiolipin in hypoglycaemia-induced apoptosis.
AuthorsNomura K, Imai H, Koumura T, Kobayashi T, Nakagawa Y
JournalBiochem J
PubMed ID10998361
Cytochrome c (cyt. c) is a proapoptotic factor that binds preferentially to cardiolipin (CL), a mitochondrial lipid, but not to cardiolipin hydroperoxide (CL-OOH). Cyt. c that had bound to CL liposomes was liberated on peroxidation of the liposomes by a radical. The generation of CL-OOH in mitochondria occurred before the ... More
Direct cardiolipin assay in yeast using the red fluorescence emission of 10-N-nonyl acridine orange.
AuthorsGallet PF, Maftah A, Petit JM, Denis-Gay M, Julien R
JournalEur J Biochem
PubMed ID7882991
The dye 10-N-nonyl-3,6-bis(dimethylamino)acridine (10-N-nonyl acridine orange) has been recently identified as a specific probe for cardiolipin (Ka = 2 x 10(6) M-1). It also interacts, at lower affinity (Ka = 7 x 10(4) M-1), with other acidic phospholipids [Petit, J. M., Maftah, A., Ratinaud, M. H. & Julien, R. (1992) ... More
Translocation of active mitochondria during hamster preimplantation embryo development studied by confocal laser scanning microscopy.
AuthorsBarnett DK, Kimura J, Bavister BD
JournalDev Dyn
PubMed ID8770552
The role of mitochondrial metabolism in the development of preimplantation embryos is unclear. To clarify the importance of mitochondria in early development, the fluorescent probes rhodamine 123 (Rh123: stains active mitochondrial membrane) and nonyl acridine orange (NAO: stains active and inactive mitochondrial membrane) were used with confocal laser scanning microscopy ... More
The apoptosis-necrosis paradox. Apoptogenic proteases activated after mitochondrial permeability transition determine the mode of cell death.
AuthorsHirsch T, Marchetti P, Susin SA, Dallaporta B, Zamzami N, Marzo I, Geuskens M, Kroemer G
JournalOncogene
PubMed ID9380409
Mitochondrial alterations including permeability transition (PT) constitute critical events of the apoptotic cascade and are under the control of Bcl-2 related gene products. Here we show that induction of PT is sufficient to activate CPP32-like proteases with DEVDase activity and the associated cleavage of the nuclear DEVDase substrate poly(ADP-ribose) polymerase ... More
Impact of proliferative activity and tumorigenic conversion on mitochondrial function of fibroblasts in 2D and 3D culture.
The purpose of the present study was to examine mitochondrial function in differently transformed cells relative to their tumorigenic state and proliferative activity in vitro. An established two-step carcinogenesis model consisting of immortal and tumorigenic rat embryo fibroblasts that can be cultured as monolayers and multicellular spheroids was investigated. Flow ... More
[Hydrophobic acridine dyes for fluorescent staining of mitochondria in living cells. 3. Specific accumulation of the fluorescent dye NAO on the mitochondrial membranes in HeLa cells by hydrophobic interaction. Depression of respiratory activity, changes in the ultrastructure of mitochondria due to NAO. Increase of fluorescence in vital stained mitochondria in situ by irradiation]
AuthorsSeptinus M, Berthold T, Naujok A, Zimmermann HW
JournalHistochemistry
PubMed ID2579934
The hydrophobic fluorescence dye 10-n-nonyl-acridinium-orange-chloride, NAO, stains specifically the mitochondria of living HeLa-cells. A dye concentration of 1 X 10(-8) M is sufficient for vital staining and at 5 X 10(-7) M an incubation time less than 1 min is enough to generate the bright green fluorescence of the mitochondria. ... More
Analysis of the membrane potential of rat- and mouse-liver mitochondria by flow cytometry and possible applications.
AuthorsPetit PX, O'Connor JE, Grunwald D, Brown SC
JournalEur J Biochem
PubMed ID2269275
Washed and purified rat- or mouse-liver mitochondria exhibiting high membrane integrity and metabolic activity were studied by flow cytometry. The electrophoretic accumulation/redistribution of cationic lipophilic probes, rhodamine 123, safranine O and a cyanine derivative, 3,3'-dihexyloxadicarbocyanine iodide, during the energization process was studied and was consistent with the generation of a ... More
Peroxidase activity and structural transitions of cytochrome c bound to cardiolipin-containing membranes.
AuthorsBelikova NA, Vladimirov YA, Osipov AN, Kapralov AA, Tyurin VA, Potapovich MV, Basova LV, Peterson J, Kurnikov IV, Kagan VE
JournalBiochemistry
PubMed ID16605268
During apoptosis, cytochrome c (cyt c) is released from intermembrane space of mitochondria into the cytosol where it triggers the caspase-dependent machinery. We discovered that cyt c plays another critical role in early apoptosis as a cardiolipin (CL)-specific oxygenase to produce CL hydroperoxides required for release of pro-apoptotic factors [Kagan, ... More
Mitochondrial proliferation and paradoxical membrane depolarization during terminal differentiation and apoptosis in a human colon carcinoma cell line.
AuthorsMancini M, Anderson BO, Caldwell E, Sedghinasab M, Paty PB, Hockenbery DM
JournalJ Cell Biol
PubMed ID9230085
Herbimycin A, a tyrosine kinase inhibitor, induces cellular differentiation and delayed apoptosis in Colo-205 cells, a poorly differentiated human colon carcinoma cell line. Cell cycle analysis in conjunction with end labeling of DNA fragments revealed that G2 arrest preceded apoptotic cell death. Ultrastructural examination of herbimycin-treated cells demonstrated morphologic features ... More
Nitric-oxide-induced apoptosis in human leukemic lines requires mitochondrial lipid degradation and cytochrome C release.
AuthorsUshmorov A, Ratter F, Lehmann V, Dröge W, Schirrmacher V, Umansky V
JournalBlood
PubMed ID10090945
We have previously shown that nitric oxide (NO) stimulates apoptosis in different human neoplastic lymphoid cell lines through activation of caspases not only via CD95/CD95L interaction, but also independently of such death receptors. Here we investigated mitochondria-dependent mechanisms of NO-induced apoptosis in Jurkat leukemic cells. NO donor glycerol trinitrate (at ... More
Influence of trans-membrane potential and of hydrophobic interactions on dye accumulation in mitochondria of living cells. Photoaffinity labelling of mitochondrial proteins, action of potential dissipating drugs, and competitive staining.
AuthorsSchneider K, Naujok A, Zimmermann HW
JournalHistochemistry
PubMed ID7960945
The lipophilic cationic fluorescent dye azopentylmethylindocarbocyanine (APMC) specifically stains the mitochondria in living cells. The dye contains a photosensitive diazirine ring and is suitable for photoaffinity labelling of mitochondrial proteins. By a combination of photoaffinity labelling cell cultures of mouse fibroblasts (LM) with APMC, lysis of the labelled cells, subsequent ... More
Staining of mitochondrial membranes with 10-nonyl acridine orange, MitoFluor Green, and MitoTracker Green is affected by mitochondrial membrane potential altering drugs.
AuthorsKeij JF, Bell-Prince C, Steinkamp JA
JournalCytometry
PubMed ID10685077
BACKGROUND: We set out to develop an assay for the simultaneous analysis of mitochondrial membrane potential and mass using the probes 10-nonyl acridine orange (NAO), MitoFluor Green (MFG), and MitoTracker Green (MTG) in HL60 cells. However, in experiments in which NAO and MFG were combined with orange emitting mitochondrial membrane ... More
Apoptosis-resistant mitochondria in T cells selected for resistance to Fas signaling.
AuthorsWang GQ, Gastman BR, Wieckowski E, Goldstein LA, Rabinovitz A, Yin XM, Rabinowich H
JournalJ Biol Chem
PubMed ID11063743
Jurkat leukemic T cells are highly sensitive to the extrinsic pathways of apoptosis induced via the death receptor Fas or tumor necrosis factor-related apoptosis-inducing ligand as well as to the intrinsic/mitochondrial pathways of death induced by VP-16 or staurosporin. We report here that clonal Jurkat cell lines selected for resistance ... More
Lack of mitochondrial anionic phospholipids causes an inhibition of translation of protein components of the electron transport chain. A yeast genetic model system for the study of anionic phospholipid function in mitochondria.
AuthorsOstrander DB, Zhang M, Mileykovskaya E, Rho M, Dowhan W
JournalJ Biol Chem
PubMed ID11335731
Reduction of mitochondrial cardiolipin (CL) levels has been postulated to compromise directly the function of several essential enzymes and processes of the mitochondria. There is limited genetic evidence for the critical roles with which CL and its precursor phosphatidylglycerol (PG) have been associated. A null allele of the PGS1 gene ... More
Increase of mitochondria and mitochondrial DNA in response to oxidative stress in human cells.
AuthorsLee HC, Yin PH, Lu CY, Chi CW, Wei YH
JournalBiochem J
PubMed ID10816438
Mitochondrial respiratory function is impaired in the target tissues of patients with mitochondrial diseases and declines with age in various human tissues. It is generally accepted that respiratory-chain defects result in enhanced production of reactive oxygen species and free radicals in mitochondria. Recently, we have demonstrated that the copy number ... More
Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses.
AuthorsDavey HM, Kell DB
JournalMicrobiol Rev
PubMed ID8987359
The most fundamental questions such as whether a cell is alive, in the sense of being able to divide or to form a colony, may sometimes be very hard to answer, since even axenic microbial cultures are extremely heterogeneous. Analyses that seek to correlate such things as viability, which is ... More
Characterization of multidrug resistance by fluorescent dyes.
AuthorsKessel D, Beck WT, Kukuruga D, Schulz V
JournalCancer Res
PubMed ID1873811
Fluorimetric techniques were used to examine accumulation of fluorescent probes by the P388 murine leukemia and an anthracycline-resistant subline, P388/Adriamycin(ADR), which expresses the multidrug-resistant phenotype. P388 could be differentiated from P388/ADR on the basis of fluorescence intensity measurements using 3 classes of cationic dyes that are sensitive to membrane potential ... More
Associated effects of divergent selection for residual feed consumption on reproduction, sperm characteristics, and mitochondria of spermatozoa.
AuthorsMorisson M, Bordas A, Petit JM, Jayat-Vignoles C, Julien R, Minvielle F
JournalPoult Sci
PubMed ID9068039
Eighteen generations of divergent selection for residual feed intake have been completed in two Rhode Island Red lines of domestic fowl. The high intake R+ line and the low intake R- line cocks used to sire Generation 19 of the selection experiment have been compared for associated responses on fertility, ... More
Subcellular localization of Photofrin and aminolevulinic acid and photodynamic cross-resistance in vitro in radiation-induced fibrosarcoma cells sensitive or resistant to photofrin-mediated photodynamic therapy.
AuthorsWilson BC, Olivo M, Singh G
JournalPhotochem Photobiol
PubMed ID9066298
The subcellular and, specifically, mitochondrial localization of the photodynamic sensitizers Photofrin and aminolevulinic acid (ALA)-induced protoporphyrin-IX (PpIX) has been investigated in vitro in radiation-induced fibrosarcoma (RIF) tumor cells. Comparisons were made of parental RIF-1 cells and cells (RIF-8A) in which resistance to Photofrin-mediated photodynamic therapy (PDT) had been induced. The ... More
Increase in cytosolic Ca2+ levels through the activation of non-selective cation channels induced by oxidative stress causes mitochondrial depolarization leading to apoptosis-like death in Leishmania donovani promastigotes.
AuthorsMukherjee SB, Das M, Sudhandiran G, Shaha C
JournalJ Biol Chem
PubMed ID11983701
Reactive oxygen species are important regulators of protozoal infection. Promastigotes of Leishmania donovani, the causative agent of Kala-azar, undergo an apoptosis-like death upon exposure to H2O2. The present study shows that upon activation of death response by H2O2, a dose- and time-dependent loss of mitochondrial membrane potential occurs. This loss ... More
Flow cytometric analysis of the early phases of apoptosis by cellular and nuclear techniques.
AuthorsFerlini C, Di Cesare S, Rainaldi G, Malorni W, Samoggia P, Biselli R, Fattorossi A
JournalCytometry
PubMed ID8725659
The early events occurring during apoptosis at the plasma membrane, chromatin, and mitochondrial levels were investigated in freshly isolated irradiated human lymphocytes, growth factor-deprived cultured human lymphocytes, and dexamethasone (DEX)-treated murine thymocytes. In intact, unfixed cells, evaluation of the light scatter properties and of DNA stainability with ethidium bromide (EB) ... More
Cardiolipin domains in Bacillus subtilis marburg membranes.
AuthorsKawai F, Shoda M, Harashima R, Sadaie Y, Hara H, Matsumoto K
JournalJ Bacteriol
PubMed ID14973018
Recently, use of the cardiolipin (CL)-specific fluorescent dye 10-N-nonyl-acridine orange (NAO) revealed CL-rich domains in the Escherichia coli membrane (E. Mileykovskaya and W. Dowhan, J. Bacteriol. 182: 1172-1175, 2000). Staining of Bacillus subtilis cells with NAO showed that there were green fluorescence domains in the septal regions and at the ... More
Mitochondrial dysfunctions in circulating T lymphocytes from human immunodeficiency virus-1 carriers.
AuthorsMacho A, Castedo M, Marchetti P, Aguilar JJ, Decaudin D, Zamzami N, Girard PM, Uriel J, Kroemer G
JournalBlood
PubMed ID7670095
In several models of lymphocyte apoptosis, two alterations of mitochondrial function precede advanced DNA fragmentation: (1) a reduction of mitochondrial transmembrane potential (delta psi m) and (2) an increase in mitochondrial generation of superoxide anion. Here we show that two fluorochromes allow for the identification of analogous mitochondrial perturbations in ... More
Sequential acquisition of mitochondrial and plasma membrane alterations during early lymphocyte apoptosis.
AuthorsCastedo M, Hirsch T, Susin SA, Zamzami N, Marchetti P, Macho A, Kroemer G
JournalJ Immunol
PubMed ID8752896
When cells undergo nuclear apoptosis (chromatin condensation, DNA fragmentation), they already manifest at least three alterations that can be quantified cytofluorometrically at the single-cell level: 1) a loss of mitochondrial transmembrane potential (delta psi m), 2) an increased production of superoxide anions, and 3) the aberrant exposure of phosphatidylserine (PS) ... More
Songorine promotes cardiac mitochondrial biogenesis via Nrf2 induction during sepsis.
Authors
JournalRedox Biol
PubMed ID33189984
SIRT3 consolidates heterochromatin and counteracts senescence.
Authors
JournalNucleic Acids Res
PubMed ID33706382
Dynamin 1-like-dependent mitochondrial fission initiates overactive mitophagy in the hepatotoxicity of cadmium.
Authors
JournalAutophagy
PubMed ID24121705
DNA polymerase gamma (Polγ) deficiency triggers a selective mTORC2 prosurvival autophagy response via mitochondria-mediated ROS signaling.