Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome.
AuthorsFusaki N, Ban H, Nishiyama A, Saeki K, Hasegawa M,
JournalProc Jpn Acad Ser B Phys Biol Sci
PubMed ID19838014
'Induced pluripotent stem cells (iPSC) have been generated from somatic cells by introducing reprogramming factors. Integration of foreign genes into the host genome is a technical hurdle for the clinical application. Here, we show that Sendai virus (SeV), an RNA virus and carries no risk of altering host genome, is ... More
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
AuthorsTakahashi K, Yamanaka S,
JournalCell
PubMed ID16904174
Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four ... More