CytoTune®-iPS Sendai Reprogramming Kit - FAQs

Invitrogen CytoTune-iPS 2.0试剂盒（货号A16517，A16518）相比已停产的原始Invitrogen CytoTune-iPS仙台病毒重编程试剂盒（货号A13780-01，A13780-02），能够为用户提供更高的重编程效率，更快的载体清除率，以及更低的细胞毒性。

2.0试剂盒包含了三个载体，其中一个是专为增强重编程效率而设计的多顺反子载体（Invitrogen CytoTune 2.0 KOS）。这一多顺反子载体具有与众不同的骨架——在聚合酶相关基因中包含了温度敏感的突变，这一突变能够帮助用户在重编程操作完成后更快地清除病毒，对细胞毒性也更小。

Invitrogen CytoTune-iPS仙台病毒重编程试剂盒（货号A13780-01，A13780-02）是一套非整合型的系统，能够将体细胞重编程为诱导性多能干细胞（iPSC）。本试剂盒利用四种仙台病毒载体，分别表达Yamanaka四因子中的一种：Oct3/4，Sox2，Klf4与c-Myc。这些转录因子在体细胞中的表达被证明是成功诱导iPSC的关键因素。

请注意Invitrogen CytoTune-iPS仙台病毒重编程试剂盒（货号A13780-01，A13780-02）已经停产，并由Invitrogen CytoTune-iPS 2.0仙台病毒重编程试剂盒（货号A16517，A16518）。  

The Invitrogen CytoTune-iPS 2.0 Kit (Cat. Nos. A16517, A16518) offers higher reprogramming efficiency, faster clearance of the vectors, and lower cytotoxicity compared to the original Invitrogen CytoTune-iPS Sendai Reprogramming Kit (Cat. Nos. A13780-01, A13780-02) that has been discontinued.

The 2.0 kit contains three vectors, one of which is a polycistronic vector (Invitrogen CytoTune 2.0 KOS), designed to deliver increased reprogramming efficiency. This polycistronic vector has a different backbone containing temperature-sensitive mutations in the polymerase-related genes, and this helps to clear the virus faster after reprogramming and causes less cytotoxicity to the cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

The Invitrogen CytoTune -iPS Sendai Reprogramming Kit (Cat. Nos. A13780-01, A13780-02) is a non-integrating system that reprograms somatic cells into induced pluripotent stem cells (iPSCs). This kit utilizes four Sendai virus-based vectors, each capable of expressing one of the four Yamanaka factors: Oct3/4, Sox2, Klf4, and c-Myc. The expression of these transcription factors in somatic cells has been shown to be a critical factor in the successful generation of iPSCs.

Please note that the Invitrogen CytoTune-iPS Sendai Reprogramming Kit (Cat. Nos. A13780-01, A13780-02) has been discontinued and replaced by the Invitrogen CytoTune-iPS 2.0 Sendai Reprogramming Kit (Cat. Nos. A16517, A16518).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.