Characterizing Pluripotent Stem Cells Using the TaqMan(®) hPSC Scorecard (TM) Panel.
AuthorsFergus J, Quintanilla R, Lakshmipathy U,
Journal
PubMed ID25138722
'Rapid technological developments for the efficient generation of footprint-free induced pluripotent stem cells (iPSC) enabled the creation of patient-specific iPSC for downstream applications in drug discovery and regenerative medicine. However, the large number of iPSCs, generated from diverse genetic backgrounds using various methods and culture conditions, created a steep challenge ... More
CRISPR/Cas9 Genome Editing of Human-Induced Pluripotent Stem Cells Followed by Granulocytic Differentiation.
AuthorsDannenmann B, Nasri M, Welte K, Skokowa J
JournalMethods Mol Biol
PubMed ID32006418
'Research on patient-derived induced pluripotent stem cells (iPSCs) could immensely benefit from the implementation of CRISPR/Cas9 genome editing of iPSCs, creating unique opportunities such as the establishment of isogenic iPSC lines for disease modeling or personalized patient-specific drug screenings. Here we describe a stepwise protocol of safe, efficient, and selection-free ... More
A Protocol for One-Step Differentiation of Human Induced Pluripotent Stem Cells into Mature Podocytes.
AuthorsMurphy C, Feifel E, Jennings P, Gstraunthaler G, Wilmes A
JournalMethods Mol Biol
PubMed ID31124107
'Within the glomerulus, podocytes are highly specialized visceral epithelial cells that are part of the glomerular filtration barrier. Human podocyte cell culture is rather challenging for primary or immortalized cells, due to the nonproliferative state of the cells. In addition, rapid dedifferentiation is often observed. Hence, iPSC-derived podocytes offer an ... More
Kinetochore Proteins Have a Post-Mitotic Function in Neurodevelopment.
AuthorsZhao G, Oztan A, Ye Y, Schwarz TL
JournalDev Cell
PubMed ID30827899
'The kinetochore is a complex of proteins, broadly conserved from yeast to man, that resides at the centromere and is essential for chromosome segregation in dividing cells. There are no known functions of the core complex outside of the centromere. We now show that the proteins of the kinetochore have ... More
Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells.
AuthorsWinston TS, Suddhapas K, Wang C, Ramos R, Soman P, Ma Z
JournalStem Cells Int
PubMed ID30766604
Combination of stem cell technology and 3D biofabrication approaches provides physiological similarity to
An Efficient Protocol for Single-Cell Cloning Human Pluripotent Stem Cells.
AuthorsSingh AM
JournalFront Cell Dev Biol
PubMed ID30766873
Genomic manipulation of human pluripotent stem cells (hPSCs) has become essential to introduce genetic modifications and transgenes, and develop reporter lines. One of the major bottlenecks in gene editing is at the stage of single-cell cloning, which is thought to be variable across hPSC lines and is substantially reduced following ... More
Human Induced Pluripotent Stem Cell-Derived Non-Cardiomyocytes Modulate Cardiac Electrophysiological Maturation Through Connexin 43-Mediated Cell-Cell Interactions.
AuthorsBiendarra-Tiegs SM, Clemens DJ, Secreto FJ, Nelson TJ
JournalStem Cells Dev
PubMed ID31744402
The functional maturation status of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) has a notable impact upon their use in pharmacological studies, disease modeling, and therapeutic applications. Non-cardiomyocytes (non-CMs) produced in the differentiation process have previously been identified as having an extrinsic influence upon hiPSC-CM development, yet the underlying mechanisms ... More