TR-FRET Cellular Assays for Interrogating Posttranslational Modifications of Histone H3.
AuthorsMachleidt T, Robers MB, Hermanson SB, Dudek JM, Bi K,
JournalJ Biomol Screen
PubMed ID21972037
'Posttranslational modifications such as phosphorylation, acetylation, and methylation play important roles in regulating the structures and functions of histones, which in turn regulate gene expression and DNA repair and replication. Histone-modifying enzymes, such as deacetylases, methyltransferases and demethylases, have been pursued as therapeutic targets for various diseases. However, detection of ... More