GeneArt® Chlamydomonas Engineering Kit - FAQs

查看更多产品信息 GeneArt® Chlamydomonas Engineering Kit - FAQs (A14258)

3 个常见问题解答

我可否使用Neon转染系统来电转哺乳动物细胞以外的细胞类型？细菌可以么？

Neon转染系统可用于电转衣藻。请参见GeneArt Chlamydomonas Protein Expression Vector (货号A24231)的说明书中推荐的电转方案。目前，我们尚未提供细菌电转的Neon实验方案。

Can I use the Neon Transfection System for the electroporation of cells other than mammalian cells? How about bacterial cells?

The Neon Transfection System may be used for electroporation of Chlamydomonas. Please refer to the GeneArt Chlamydomonas Protein Expression Vector (Cat. No. A24231) manual for Neon instructions. Currently, we do not offer a Neon protocol for electroporation of bacterial cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Do you have a Chlamydomonas transformation protocol using the Neon Transfection System?

We recommend the following guidelines:

- For high transformation efficiencies, the OD750 of the C. reinhardtii culture should be > 0.8 before electroporation.
- Carry out all transformation steps at 4 degrees C using solutions pre-equilibrated at 4 degrees C.
- Electroporate the cells using the 100 µL Neon tip in TAP-40 mM sucrose solution or the MAX Efficiency Transformation Reagent For Algae (Cat. no. A24229) at 4 degrees C.
- Use the following Neon electroporation parameters: 2300 volts (Voltage), 13 ms (Pulse Width), 3 (Pulse Number)

For detailed instructions on using the Neon Transfection System, refer to the Neon Transfection System User Guide (https://tools.thermofisher.com/content/sfs/manuals/neon_device_man.pdf).

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.