A STUDY OF SPONTANEOUS MINIATURE POTENTIALS IN SPINAL MOTONEURONES.
AuthorsKATZ B, MILEDI R,
JournalJ Physiol
PubMed ID14062684
An investigation of spontaneous activity at the neuromuscular junction of the rat.
AuthorsLILEY AW,
JournalJ Physiol
PubMed ID13332600
Regarding the Action of Hydrate of Soda, Hydrate of Ammonia, and Hydrate of Potash on the Ventricle of the Frog's Heart.
AuthorsRinger S,
JournalJ Physiol
PubMed ID16991317
Potassium channels in myelinated nerve. Selective permeability to small cations.
AuthorsHille B,
JournalJ Gen Physiol
PubMed ID4541077
Activation of the endoplasmic reticulum calcium sensor STIM1 and store-operated calcium entry by rotavirus requires NSP4 viroporin activity.
AuthorsHyser JM, Utama B, Crawford SE, Broughman JR, Estes MK,
Journal
PubMed ID24109210
Rotavirus nonstructural protein 4 (NSP4) induces dramatic changes in cellular calcium homeostasis. These include increased endoplasmic reticulum (ER) permeability, resulting in decreased ER calcium stores and activation of plasma membrane (PM) calcium influx channels, ultimately causing a 2- to 4-fold elevation in cytoplasmic calcium. Elevated cytoplasmic calcium is absolutely required ... More
Stimulated Emission Depletion (STED) Imaging of Clathrin-Mediated Endocytosis in Living Cells.
AuthorsBottanelli F, Schroeder L
JournalMethods Mol Biol
PubMed ID30129018
'The recent development of probes and labeling strategies for multicolor super-resolution imaging in living cells allows cell biologists to follow cellular processes with unprecedented details. Here we describe how to image endocytic events at the plasma membrane of living cells using commercial (Leica, Abberior Instruments) or custom built STED microscopes.' ... More
High-Resolution Live-Cell Imaging and Analysis by Laser Desorption/Ionization Droplet Delivery Mass Spectrometry.
AuthorsLee JK, Jansson ET, Nam HG, Zare RN
JournalAnal Chem
PubMed ID27110027
We have developed a new ambient-ionization mass spectrometric technique named laser desorption/ionization droplet delivery mass spectrometry (LDIDD-MS). LDIDD-MS permits high-resolution, high-sensitivity imaging of tissue samples as well as measurements of both single-cell apoptosis and live-cell exocytosis. A pulsed (15 Hz) UV laser beam (266 nm) is focused on a surface ... More
Long-Term Live Cell Imaging of Cell Migration: Effects of Pathogenic Fungi on Human Epithelial Cell Migration.
AuthorsWöllert T, Langford GM
JournalMethods Mol Biol
PubMed ID26498777
Long-term live cell imaging was used in this study to determine the responses of human epithelial cells to pathogenic biofilms formed by Candida albicans. Epithelial cells of the skin represent the front line of defense against invasive pathogens such as C. albicans but under certain circumstances, especially when the host's ... More
A fluorescent probe for cysteine depalmitoylation reveals dynamic APT signaling.
AuthorsKathayat RS, Elvira PD, Dickinson BC
JournalNat Chem Biol
PubMed ID27992880
Hundreds of human proteins are modified by reversible palmitoylation of cysteine residues (S-palmitoylation), but the regulation of depalmitoylation is poorly understood. Here, we develop 'depalmitoylation probes' (DPPs), small-molecule fluorophores, to monitor the endogenous activity levels of 'erasers' of S-palmitoylation, acylprotein thioesterases (APTs). Live-cell analysis with DPPs reveals rapid growth-factor-mediated inhibition ... More
Live-cell imaging of human spermatozoa using structured illumination microscopy.
AuthorsOpstad IS, Popova DA, Acharya G, Basnet P, Ahluwalia BS
JournalBiomed Opt Express
PubMed ID31065404
Structural details of spermatozoa are interesting from the perspectives of fundamental biology and growing reproductive health problems. Studies of nanostructural details of these extremely motile cells have been limited to fixed cells, largely using electron microscopy. Here we provide the protocols for and demonstrate live-cell multi-color super-resolution imaging of human ... More
Visualizing RNA in Live Bacterial Cells Using Fluorophore- and Quencher-Binding Aptamers.
AuthorsSunbul M, Arora A, Jäschke A
JournalMethods Mol Biol
PubMed ID29130205
To elucidate the roles, dynamics, and regulation of RNAs, it is vital to be able to visualize the RNA of interest (ROI) in living cells noninvasively. Here, we describe a novel live-cell RNA imaging method using fluorophore- and quencher-binding aptamers, which can be genetically fused to the ROI. In this ... More
Measuring S-Depalmitoylation Activity In Vitro and In Live Cells with Fluorescent Probes.
AuthorsKathayat RS, Dickinson BC
JournalMethods Mol Biol
PubMed ID31152398
S-palmitoylation is a reversible lipid posttranslational modification (PTM) that can mediate protein localization, trafficking, interaction with membranes, and a host of other biophysical characteristics. Over the past decade, a suite of chemoproteomic strategies have uncovered the breadth of S-palmitoylation, revealing widespread susceptibility to modification by this PTM throughout the human ... More
A simple microfluidic device for live-imaging of the vertical section of epithelial cells.
AuthorsAraki S, Nakano M, Tsugane M, Sunaga F, Hattori M, Nakano M, Nagai T, Suzuki H
JournalAnalyst
PubMed ID31799546
We investigated the capability of simple microfluidic devices with trenches having vertical sidewalls for live-cell fluorescence imaging of adherent cells. An epithelial cell line that forms a two-dimensional (2D) sheet was cultured to adhere to the vertical sidewall so that its vertical section can be imaged directly using ordinal inverted-type ... More