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Thermo Scientific Chemicals
5,5'-二硫双(2-硝基苯甲酸), 99%
CAS: 69-78-3 | C14H8N2O8S2 | 396.34 g/mol
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化学标识符
CAS69-78-3
规格
规格表
规格表Appearance (Color)Pale yellow to pale cream
FormPowder
Assay (Aqueous acid-base Titration)≥98.5 to ≤101.5%
Assay (HPLC)≥98.5%
Identification (FTIR)Conforms
Reagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Reagent for determination of sufhydryl groups.5,5′-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content.
Solubility
Soluble in water and ethanol.
Notes
Keep the container tightly closed in a dry and well-ventilated place. Incompatible with strong oxidizing agents and strong bases.
Reagent for determination of sufhydryl groups.5,5′-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content.
Solubility
Soluble in water and ethanol.
Notes
Keep the container tightly closed in a dry and well-ventilated place. Incompatible with strong oxidizing agents and strong bases.
RUO – Research Use Only
General References:
- Reacts with free thiol groups to form a highly colored (yellow) thiolate anion: Arch. Biochem. Biophys., 82, 70 (1959); used for quantitative determination of thiols. Also used in chromogenic assay of acetylcholinesterase: Enzyme Assays, R. Eisenthal and M. J. Danson, Eds., OUP, Oxford (1992), p81.
- Titi, H. M.; Nandi, G.; Thakuria, R.; Goldberg, I. Coordination polymers of 5,5'-dithiobis(2-nitrobenzoic acid): Synthesis, structure and topology. Inorg. Chim. Acta 2015, 426, 55-63.
- Li, Z. G. Chapter Six - Quantification of Hydrogen Sulfide Concentration Using Methylene Blue and 5,5'-Dithiobis(2-Nitrobenzoic Acid) Methods in Plants. Methods Enzymol. 2015, 554, 101-110.