Question 1

我能使用ProBond或Ni-NTA纯化系统来纯化Expi293表达培养基中的目的蛋白吗？

Accepted Answer

Expi293表达培养基并不直接兼容ProBond或Ni-NTA纯化系统。我们推荐在用His标签的纯化操作之前进行一次缓冲体系交换或样本透析。

Question 2

Can I purify my protein from Expi293 Expression Medium using ProBond or Ni-NTA purification systems?

Accepted Answer

Expi293 Expression Medium is not directly compatible with ProBond or Ni-NTA purification systems. We recommend performing a buffer exchange or dialyzing the samples before His-tag purification.

Question 3

Can I shake Expi293 cultures faster or slower than recommended?

Accepted Answer

For most flasks, there is a dropoff in expression when you go too fast or too slow because of cell shear stress or insufficient aeration. The effect is greater in plates where there is a sharper transition between static and moving fluid in the wells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Question 4

My Expi293 Expression Medium (Cat. No. A1435102) was accidentally frozen. Can I still use it?

Accepted Answer

This product should be stored at 2-8 degrees C and should not be frozen. The biggest issue with accidentally freezing this product is its solubility. If this product was accidentally frozen, we recommend placing it in a 2-8 degree environment and allowing it to slowly thaw overnight. If the product is fully thawed and shows no signs of precipitation, then it should still be usable, but we cannot guarantee effectiveness.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Question 5

Does Expi293 Expression Medium contain antibiotics?

Accepted Answer

Expi293 Expression Medium does not contain antibiotics. We do not recommend the use of antibiotics, however, 5 mL/L of Antibiotic-Antimycotic (Cat. No. 15240) containing penicillin, streptomycin, and amphotericin B may be used when required, as stated on page 1 in the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/expi293_exp_medium_man.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Question 6

Do I need to remove Anti-Clumping Agent and Pluronic F-68 from the medium while growing Expi293 cells? How about during transfection of these cells?

Accepted Answer

You can have Anti-Clumping Agent and Pluronic F-68 in the medium while growing Expi293 cells. You need to remove Anti-Clumping Agent at least 2 passages prior to transfection of Expi293 cells because it interferes with transfection. You can add it back to the medium after transfection. Pluoronic F-68 can be present during transfection as it does not interfere with transfection.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Expi293™ Expression Medium, 10 L - FAQs