iPSC克隆可通过碱性磷酸酶染色法轻松实现可视化,相关试剂盒有碱性磷酸酶活细胞染色试剂盒(Alkaline Phosphatase Live Stain,货号 A14353)。此外,重编程克隆可通过Tra1-60或Tra1-81抗体的活细胞染色来进行筛选,这些抗体能够识别未分化的iPSC,进而帮助用户从各种人体细胞中鉴定出重编程细胞。请参考用户手册(https://tools.thermofisher.com/content/sfs/manuals/AP_Live_Stain_man.pdf)获取完整的实验方案。
How can I prevent poor or dim staining when using Alkaline Phosphatase Live Stain?
Serum or serum replacement components in the growth medium may cause background and this can result in poor or dim staining. After the removal of the growth medium, gently wash the culture with pre-warmed DMEM/F-12 (Cat. No. 10565-018) for 5 minutes. Aspirate and repeat once before adding the AP Live Stain. To further decrease background staining, perform 3 separate washes of 5 minutes each with DMEM/F-12 for a total of 15 minutes following the staining, and visualize immediately.
How should Alkaline Phosphatase Live Stain be stored?
Store AP Live Stain at -20 degrees C in the freezer and thaw at room temperature. Avoid repeated freeze/thaw cycles and aliquot the AP Live Stain into smaller volumes if necessary. Always protect from light and avoid extended exposure at room temperature and atmospheric conditions.
Can I use Alkaline Phosphatase Live Stain in my reprogramming experiments?
AP Live Stain is ideal for screening colonies during early stages of reprogramming since it selectively stains PSCs while maintaining cell viability. It can be used in later stages of reprogramming to identify undifferentiated cells for the selection of iPSCs for further cultivation. AP Live Stain was developed specifically for use on live cultures for cell maintenance and is qualified to be free of mycoplasma and bioburden, and exhibits extremely low endotoxin levels.
How long should I wait before using Alkaline Phosphatase Live stain on my cells again?
Colonies may be restained as early as 24 hours after the initial staining. It is recommended that cells are allowed to recover properly to obtain consistent results.