ExpiFectamine™ 293 Transfection Kit

The ExpiFectamine 293 Transfection Kit is a core component of the Expi293 Expression System. It is designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells. The high-efficiency, cationic, lipid-based transfection reagent and transfection enhancers are designed to power the highest possible level of protein expression from Expi293F cells cultured in Expi293 Expression Medium.

• Designed specifically for transfection of high density suspension cell culture, with matching transfection enhancers that boost transfection performance and protein expression
• Achieves protein yields 2- to 10-fold higher than other transfection reagents used on high density 293 cell cultures
• Employs the same transient expression protocols typically used in current low-density 293 suspension culture systems to easily switch from low density systems to the high yield, high density Expi293 Expression System
• Provides robust and reproducible transfection results, giving users greater confidence in their results
• Scale transfections for culture volumes of less than 1 mL to greater than 10 L, while maintaining equivalent volumetric protein yields

The ExpiFectamine 293 Transfection Kit is specifically designed to work with Expi293F cells in the Expi293 Expression System. The transfection kit consists of the cationic lipid-based ExpiFectamine 293 Reagent and ExpiFectamine 293 Transfection Enhancers 1 and 2. The enhancers are added 18–22 hours post-transfection to boost transfection, cell viability, and protein expression. The protocol for use of ExpiFectamine 293 Transfection components is very similar to transfection protocols for other transient expression reagents, such as 293fectin and FreeStyle MAX.