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Gibco™
Expi293™ Expression System Kit
Designed with transient expression technology for rapid and ultra high-yield protein production from mammalian cells.
| Catalog Number | Quantity |
|---|---|
| A14635 | 1 Kit |
Catalog number A14635
Price (CNY)
33,476.00
Each
Quantity:
1 Kit
Price (CNY)
33,476.00
Each
The Expi293 Expression System is a complete, scalable platform for rapid, high-yield transient protein production from mammalian HEK293 cells. The system includes: high-density Expi293F cells in Expi293 Expression Medium and ExpiFectamine 293 transfection reagent optimized with enhancers to maximize protein production and reproducibility.
- Complete kit—contains all components needed for efficient, recombinant protein expression with easy-to-follow culture and transfection protocols
- Up to 1 g/L of protein—2- to 10-fold higher protein yields than previous generation transient expression systems, such as the FreeStyle 293 Expression System
- Rapid production—rapid expression of recombinant proteins in just 2–7 days for a wide variety of protein formats from integral membrane proteins to monoclonal antibodies
- High density cultures—high-density Expi293F cultures yield significantly more protein-expressing cells per mL, boosting expression efficiency
- Native folding and mammalian post-translational modifications—proteins are expressed from human HEK293 cells resulting in a high degree of glycosylation and quality
- Scalable expression from <1 mL to >10 L—easily scalable and automation friendly from 96 deepwell plates for high throughput applications to multi-liter bioreactor platforms
The components included in the Expi293 Expression System Kit are: two vials of frozen Expi293F cells, one liter of Expi293 Expression Medium, one ExpiFectamine 293 Transfection kit sufficient to transfect up to 1 liter of final culture, Opti-MEM I Reduced Serum Medium complexation buffer, and an antibody-expressing positive control vector. Expi293 Transfection kits are also available in large-scale formats for transfecting up to 10 L and 50 L of Expi293 cells. Custom formats and volumes are also available upon request.
Engineered Expi293F cell lines are available for a range of structural biology applications, including Cryo-EM, NMR, and X-ray crystallography. Available individually or in kits.
- Expi293F GnTI- Cells (Cat. No. A39240)
- Expi293F Inducible Cells (Cat. No. A39241)
- Expi293F Inducible GnTI- Cells (Cat. No. A39242)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineExpi293F™
Delivery TypeTransfection
Expression StabilityTransient
Expression SystemMammalian
For Use With (Application)Protein Expression
Key FunctionsProtein Production, Constitutive Expression
No. of Reactions33
Product LineExpi293™
Product TypeExpression System Kit
Protein TagUntagged
Quantity1 Kit
System TypeExpi293™ Expression System
Cell Type293-derived Cells
PromoterCMV
Transfection TechniqueLipid-based Transfection
Unit SizeEach
Contents & Storage
2 vials frozen Expi293F cells
1 bottle 1000 mL Expi293 Expression Medium
1 ExpiFectamine 293 transfection kit for 1 L of culture
1 vial Antibody Expressing Positive Control Vector
1 bottle 100 mL Opti-MEM Reduced Serum Medium
Store all components at +2°C to +8°C, except frozen cells are stored in liquid nitrogen
1 bottle 1000 mL Expi293 Expression Medium
1 ExpiFectamine 293 transfection kit for 1 L of culture
1 vial Antibody Expressing Positive Control Vector
1 bottle 100 mL Opti-MEM Reduced Serum Medium
Store all components at +2°C to +8°C, except frozen cells are stored in liquid nitrogen