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Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We do not recommend thawing at 37 degrees C. Best results are observed upon thawing at room temperature for approximately 2 hrs or overnight at 2-8 degrees C.Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We recommend thawing it at 4 degrees C overnight or at room temperature for 2 hours. We don't recommend thawing in a water bath or incubator.Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Yes, we recommend that you aliquot when you first thaw it and thereafter subject the aliquot to no more than 2 additional freeze/thaw cycles.Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.