Low Input RiboMinus™ Eukaryote System v2 - FAQs

How does the Low Input RiboMinus Eukaryote System v2 differ compare to the RiboMinus Eukaryote Kit for RNA-seq?

The Low Input RiboMinus Eukaryote kit improves upon the previous system (RiboMinus Eukaryote Kit for RNA-Seq) by removing significantly more rRNA (both cytoplasmic and mitochondrial) from the total RNA. This system provides maximum mapping to reference database with minimum bias, maintaining relative gene expression levels of even smaller RNA species. The resulting total RNA after depletion contains minimum contaminating rRNA with reduced variability.

The Low Input RiboMinus Eukaryote System v2 is designed for the removal of human, mouse, and rat rRNA. A magnetic bead-based purification module is included for purification of the whole transcriptome without altering the expression levels of small or non-coding RNA.