Effect of diacylglycerol content on some physicochemical properties of the insect lipoprotein, lipophorin. Correlation with the binding of apolipophorin-III.
AuthorsSoulages JL, Wells MA
JournalBiochemistry
PubMed ID8117694
'Diacylglycerol (DG) is the main lipid component of the insect lipoprotein lipophorin. In order to study the effect of DG content on the structure and properties of lipophorin and to analyze the role of DG in the binding of apolipophorin-III (apoLp-III), an exchangeable apolipoprotein, we developed a method that allows ... More
Photoactivated cross-linking of proteins within the erythrocyte membrane core.
AuthorsMikkelsen RB, Wallach DF
JournalJ Biol Chem
PubMed ID12163
'We describe the reactions of three lipophilic, photoactivated cross-linking reagents, 1,5-diazidonapthalene, 4,4''-diazidobiphenyl, and the reversible 4,4''-dithiobisphenylazide, with erythrocyte membranes. Cross-linking occurs only upon photoactivation. At pH 7 to 8, only spectrin components are cross-linked by these reagents. At pH 5.0 to 5.5 several additional membrane proteins including the major "integral" ... More
The alpha and gamma subunits of initiation factor eIF-2 can be cross-linked to 18S ribosomal RNA within the quaternary initiation complex, eIF-2.Met-tRNAf.GDPCP.small ribosomal subunit.
AuthorsWestermann P, Nygård O, Bielka H
JournalNucleic Acids Res
PubMed ID7443514
'Initiation factor eIF-2 from rat liver was reacted with the hetero-bifunctional cross-linking reagents ABAI or APTPI without diminishing its ability to form the quaternary initiation complex with Met-tRNAf, GDPCP and the small ribosomal subunit. Upon irradiation with UV light, subunits alpha and gamma of eIF-2 became covalently linked to 18S ... More
Localization of membrane-associated proteins in vesicular stomatitis virus by use of hydrophobic membrane probes and cross-linking reagents.
AuthorsZakowski JJ, Wagner RR
JournalJ Virol
PubMed ID6255216
'The location of membrane-associated proteins of vesicular stomatitis virus was investigated by using two monofunctional and three bifunctional probes that differ in the degree to which they partition into membranes and in their specific group reactivity. Two hydrophobic aryl azide probes, [(125)I]5-iodonaphthyl-1-azide and [(3)H]pyrenesulfonylazide, readily partitioned into virion membrane and, ... More
Building a replisome solution structure by elucidation of protein-protein interactions in the bacteriophage T4 DNA polymerase holoenzyme.
'Assembly of DNA replication systems requires the coordinated actions of many proteins. The multiprotein complexes formed as intermediates on the pathway to the final DNA polymerase holoenzyme have been shown to have distinct structures relative to the ground-state structures of the individual proteins. By using a variety of solution-phase techniques, ... More
Synthesis and application of cleavable photoactivable heterobifunctional reagents.
AuthorsVanin EF, Ji TH
JournalBiochemistry
PubMed ID7317350
'Three different cleavable photoactivable heterobifunctional reagents have been synthesized and characterized by proton NMR, 13C NMR, mass spectroscopy, infrared spectroscopy, and thin-layer chromatography. They are the N-hydroxysuccinimide ester of 3-[(4-azidophenyl)dithio]-propionic acid, methyl 3-[(4-azidophenyl)dithio]propionimidate, and N-[(4-azidophenyl)thio]phthalimide. Concanavalin A was coupled with one of the reagents, the N-hydroxysuccinimide ester of 3-[(4-azidophenyl)dithio]propionic acid, ... More
Chemical modification of proteins: comments and perspectives.
AuthorsFeeney RE
JournalInt J Pept Protein Res
PubMed ID3570660
'The use of chemical modification of proteins has increased exponentially during the past two decades. Today the many different uses of chemical modification include determination of relative reactivities of side chain groups, the quantitation of individual amino acids, development of affinity reagents, mechanism-based reagents for pharmaceutical uses, cross-linking reagents, special ... More
Photochemical cross-linking of cell membranes. A test for natural and random collisional cross-links by millisecond cross-linking.
AuthorsKiehm DJ, Ji TH
JournalJ Biol Chem
PubMed ID925009
Synthesis and characterization of N-(4-azidophenylthio)phthalimide: A cleavable, photoactivable crosslinking reagent that reacts with sulfhydryl groups.
AuthorsMoreland RB, Smith PK, Fujimoto EK, Dockter ME
JournalAnal Biochem
PubMed ID6285759
A photochemical crosslinking study of the subunit structure of membrane-associated spectrin.
AuthorsMiddaugh CR, Ji TH
JournalEur J Biochem
PubMed ID7439176
The subunit structure of membrane-associated spectrin was investigated by crosslinking human erythrocyte membranes with a variety of cleavable photosensitive heterobifunctional reagents by flash photolysis millisecond crosslinking. Crosslinked complexes were analyzed on agarose-polyacrylamide gels whose high exclusion limit the resolution of molecular with Mr larger than 10(6). Crosslinking of membrane ghosts ... More