Essential 6™ Medium - FAQs

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6 product FAQs found

我应如何在体外培养人诱导性多能干（iPS）细胞？

人iPS与人ES细胞的生长条件相同。与人ES细胞类似，人iPS细胞能够生长于辐照的MEF滋养层细胞上+含20% KnockOut SR，0.1 mM NEAA，1mM谷氨酰胺，0.1mM b-ME的DMEM/F12培养基 + 辐照MEF饲养细胞 + 100 ng/mL bFGF的培养条件中，或Matrigel基质包被培养皿 + MEF条件培养基 + 100 ng/mL bFGF的培养条件中（Science318:1917 (2007)）。人ES细胞与iPS细胞两者均可生长于KnockOut ESC/iPS培养基中（货号A14131）。

人胚胎干（ES）细胞研究中一般使用何种生长因子？

用于细胞培养：人重组激活化素A（货号PHC9564），bFGF（货号PHG0261），IGF-II（货号PHG0084）（Stem Cells 24:1476 (2006); Nature 448:1015 (2007)）。
用于人ES细胞分化：BMP-4（货号PHC9533），EGF（货号PHG0311）和HGF（货号PHG0324）（Proc Natl Acad Sci U S A 97:11307 (2000)）。

Can I store Gibco Essential 6 Medium (Cat. No. A1516401) at -20 degrees C?

We do not recommend freezing our liquid media products. Freezing can cause some components like amino acids and inorganic salts to precipitate upon thawing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it possible to reprogram somatic cells using Essential 7 or Essential 8 medium and transition them into StemFlex Medium upon harvest?

Yes. If improved reprogramming efficiency is required, then you may utilize Essential 7 (Essential 6 + bFGF) or Essential 8 Medium for reprogramming somatic cells and directly transition them into StemFlex Medium system upon colony selection.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

When should I use the full length Human FGF-basic (FGF-2/bFGF) Recombinant Protein or the truncated variant, Human FGF-basic (FGF-2/bFGF) (aa 10-155) Recombinant Protein?

The full length Human FGF-basic (FGF-2/bFGF) (aa 1-155) Recombinant Protein is recommended for stem cells whereas the truncated variant, Human FGF-basic (FGF-2/bFGF) (aa 10-155) Recombinant Protein which is missing the first 9 amino acids, is recommended for use with neural and cardiac cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are induced pluripotent stem cells?

Induced pluripotent stem cells (iPS or iPSCs) are pluripotent stem cells directly generated by introducing combination of genes coding for “reprogramming factors” into adult cells. These reprogramming factors include Oct4, Sox2, c-Myc, KLF4, NANOG, and LIN28. Yu, et al, generated iPS from a human mesenchymal cell line using lentiviral vectors carrying Oct4, Sox2, NANOG, and LIN28 genes (Science 318:1917 (2007)). Using a similar approach, Takahashi et al, generated iPS from human primary fibroblast cells by introducing genes coding for Oct3, Sox2, KLF4, and c-Myc into these cells (Cell 131:861 (2007)). iPS generated by reprogramming are similar to human ES cells in morphology, the capacity for unlimited proliferation, surface-antigen expression, gene expression, the ability to differentiate into cell types representing the three germ layers in vitro, and the ability to form teratomas after injection into SCID mice.