原代小鼠海马神经元

Name: 原代小鼠海马神经元
SKU: A15587

Gibco Primary Mouse Hippocampal Neurons are isolated and cryopreserved from C57BL/6 embryonic day-17 mice. A flexible, ready-to-use, and quality alternative了解更多信息

货号 A15587

价格（CNY)

Gibco Primary Mouse Hippocampal Neurons are isolated and cryopreserved from C57BL/6 embryonic day-17 mice. A flexible, ready-to-use, and quality alternative to fresh neurons, each vial contains 1 million cells per mL. These neurons consistently yield post-thaw viability in the range of 50-80%, have high neuronal purity, and are validated for functionality.

• High viability: post-thaw viability of 50% or greater
• High purity: greater than 98% neuronal phenotype
• Functionally validated: tested for long-term survival, neurotransmitter response, and transduction

For Research Use Only. Not for use in diagnostic procedures.

规格

池数量1百万

产品类型小鼠海马神经元

数量1 X 10⁶ 细胞

备注小鼠品系为 C57BL/6

经过测试无菌性、存活率、支原体

年龄胎儿

细胞类型神经细胞

培养类型贴壁细胞培养

形式Cryopreserved

种属Mouse

组织海马

Unit SizeEach

常见问题解答 (FAQ)

How were the Primary Mouse Hippocampal Neurons isolated? How specific was the dissection area (does it include the dentate gyrus, CA1, CA2, and/or CA3)? How much glutamate should I use for culturing them?

The neurons isolated are likely from all the areas of the hippocampus you listed. The glutamate is used to establish the culture. If the media is being changed every day, the media should have glutamate supplemented through day 4 after recovery. Beyond day 4, it is not necessary and likely not preferred. Any glutamate can be used as long as it is of a higher purity such as FCC, USP, ACS, etc. grade.

I'm currently culturing neurons. Which media system do you recommend using in conjunction with the Poly-D-Lysine matrix?

We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What cell types will Poly-D-Lysine support as an attachment matrix?

The Poly-D-Lysine matrix has successfully supported the attachment of primary rodent neurons, iPSC-derived neurons and PC12 cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.