TaqMan™ hPSC Scorecard™ 检测板，快速 96 孔

引用和文献 (20)

Applied Biosystems™

TaqMan™ hPSC Scorecard™ 检测板，快速 96 孔

Applied Biosystems™ TaqMan™ hPSC Scorecard™ 检测板 2 x 96w FAST了解更多信息

货号	数量
A15876	96 次反应

货号 A15876

价格（CNY)

10,615.26

Ends: 31-Dec-2025

12,650.00

共减 2,034.74 (16%)

添加至购物车

96 次反应

价格（CNY)

10,615.26

Ends: 31-Dec-2025

12,650.00

共减 2,034.74 (16%)

添加至购物车

Applied Biosystems™ TaqMan™ hPSC Scorecard™ 检测板 2 x 96w FAST 可验证多能性以及测定 ES 和 iPS 细胞系的谱系偏倚。两个 96 孔板各包含 94 个孔中干燥的预定 TaqMan™ 基因表达检测（包括内源性对照）。预混液不包括在此检测板中。有关检测板与预混液组合的试剂盒，请参见 TaqMan™ hPSC Scorecard™ 试剂盒 2 x 96w FAST。该产品还提供 384 孔规格。

•在一次简单的实验中评估整个基因标签
• 将结果与随附的 hPSC Scorecard™ 分析软件中的参考标准进行比较
•确定具有三系分化潜能的细胞系
•评价特定于一个胚层的诱导分化
•访问经验证的内容，增加结果的可信度

在一次实验中评估整个基因标签
TaqMan™ hPSC Scorecard™ 检测组合能以稳定、便利的干燥形式提供预铺平板测定，有助于节省时间。只需将 TaqMan™ 预混液添加到您的目的 cDNA 中，并使用多通道移液器将其转移到 96 孔板中。

将结果与参考标准进行比较
TaqMan™ hPSC Scorecard™ 检测组合包括访问基于云计算的专有分析软件，使您可以查看和导出结果的图形和表格，以及生成报告。该软件与七个 Applied Biosystems™ qRT-PCR 系统兼容，无需额外收费。

鉴定具有三系分化潜能的细胞系
将随机分化的拟胚体 (EB) 样品（使用标准 EB 方法分化至少 7 天）包括在您的分析中，并确定您的细胞系是否偏向于三胚层（内胚层、中胚层、外胚层）中的一层或多层。

评价特定于一个胚层的诱导分化
检测组合包括超过用于三个胚层中每个胚层的 20 多个标志物，这些胚层都已显示对单层培养中的定向分化的响应与预期一致。利用这一检测板快速评价目的胚层的时间进程数据、培养条件和培养基配方。

访问经验证内容
检测板内容基于已发表的工作（Bock 等人，Cell 144，439–452，2011），并对照多个人 ES 和 iPS 细胞系进行了验证。

产品包装规格
TaqMan™ hPSC Scorecard™ 检测板 2 x 96w FAST 包括：

•包含 94 个 TaqMan™ 基因表达检测的两个 96 孔板
•两个光学板盖

仅供科研使用。不可用于诊断程序。

检测方法引物-探针检测

适用于（设备）QuantStudio™ 12k Flex，StepOne™，快速模式，ViiA™ 7 系统

包括2 x 96 孔板

产品线TaqMan

数量96 次反应

反应速度快速

研究类别干细胞研究

类型检测组合

产品规格96 阵列板

Unit SizeEach

内容与储存

目录：两个 96 孔板，各包含 96 个检测板和两个光学盖

在室温下储存。

常见问题解答 (FAQ)

I'm using the TaqMan hPSC Scorecard Panel. How do I load the samples onto a 384-well plate if I don't have a multichannel pipette?

You can load samples using a single channel pipette, but this method is time-consuming and may increase pipetting error. We strongly recommend that you use an 8- or 16-channel multichannel pipette. You also can dispense samples using automated systems, with the understanding that additional sample will be required to compensate for the dead volume.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center

Why should I set up cDNA synthesis in 8 wells of a 96-well plate or in 8-well PCR strips when using the TaqMan hPSC Scorecard Panel?

Setting up your cDNA synthesis in 8 wells of a 96-well plate or in 8-well PCR strips facilitates sample loading of the 96-well and 384-well hPSC Scorecard Panel plates.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center

Can I analyze somatic non-pluripotent primary cells with the TaqMan hPSC Scorecard Panel?

Somatic non-pluripotent primary cells, such as the parental lines used for iPSC generation, are not pluripotent in nature and their scores will be low. However, the expression of lineage markers will largely rely on homogeneity of the cells. Note that the markers in the panel are designed to evaluate early germ layer specification and not any particular terminally differentiated state.

Does the reprogramming method affect the TaqMan hPSC Scorecard analysis results?

Minor differences in gene expression profiles are sometimes observed based on the reprogramming method, but in general the hPSC Scorecard analysis results do not change significantly for lines derived using various reprogramming methods. This was tested with ESC and iPS derived using episomal or Sendai-based reprogramming systems before and after seven days of spontaneous differentiation. Cells were grown in KSR-based media on irradiated MEFs prior to removal of FGF for EB formation.

Will the presence of Sendai virus affect my TaqMan hPSC Scorecard analysis results?

The presence or absence of Sendai virus in established iPSC clones does not have an impact on pluripotency and hence on TaqMan hPSC Scorecard analysis results.

View all TaqMan™ hPSC Scorecard™ 检测板，快速 96 孔 FAQs

引用和文献 (20)

引用和文献

Abstract

Characterizing Pluripotent Stem Cells Using the TaqMan(®) hPSC Scorecard (TM) Panel.

Authors:Fergus J, Quintanilla R, Lakshmipathy U,

Journal:

PubMed ID:25138722

'Rapid technological developments for the efficient generation of footprint-free induced pluripotent stem cells (iPSC) enabled the creation of patient-specific iPSC for downstream applications in drug discovery and regenerative medicine. However, the large number of iPSCs, generated from diverse genetic backgrounds using various methods and culture conditions, created a steep challenge ... More

Generation of iPSCs as a Pooled Culture Using Magnetic Activated Cell Sorting of Newly Reprogrammed Cells.

Authors:Yang W, Liu Y, Slovik KJ, Wu JC, Duncan SA, Rader DJ, Morrisey EE,

Journal:

PubMed ID:26281015

'Although significant advancement has been made in the induced pluripotent stem cell (iPSC) field, current methods for iPSC derivation are labor intensive and costly. These methods involve manual selection, expansion, and characterization of multiple clones for each reprogrammed cell sample and therefore significantly hampers the feasibility of studies where a ... More

Efficient Generation of Induced Pluripotent Stem and Neural Progenitor Cells From Acutely Harvested Dura Mater Obtained During Ventriculoperitoneal Shunt Surgery.

Authors:Cary WA, Hori CN, Pham MT, Nacey CA, McGee JL, Hamou M, Berman RF, Bauer G, Nolta JA, Waldau B,

Journal:

PubMed ID:26074438

'The dura mater can be easily biopsied during most cranial neurosurgical operations. We describe a protocol that allows for robust generation of induced pluripotent stem cells (iPSCs) and neural progenitors from acutely harvested dura mater. To generate iPSCs and neural progenitor cells from dura mater obtained during ventriculoperitoneal shunt surgery. ... More

Integrative Analyses of Human Reprogramming Reveal Dynamic Nature of Induced Pluripotency.

Authors:Cacchiarelli D, Trapnell C, Ziller MJ, Soumillon M, Cesana M, Karnik R, Donaghey J, Smith ZD, Ratanasirintrawoot S, Zhang X, Ho Sui SJ, Wu Z, Akopian V, Gifford CA, Doench J, Rinn JL, Daley GQ, Meissner A, Lander ES, Mikkelsen TS,

Journal:

PubMed ID:26186193

'Induced pluripotency is a promising avenue for disease modeling and therapy, but the molecular principles underlying this process, particularly in human cells, remain poorly understood due to donor-to-donor variability and intercellular heterogeneity. Here, we constructed and characterized a clonal, inducible human reprogramming system that provides a reliable source of cells ... More

Transcription factor binding dynamics during human ES cell differentiation.

Authors:Tsankov AM, Gu H, Akopian V, Ziller MJ, Donaghey J, Amit I, Gnirke A, Meissner A,

Journal:

PubMed ID:25693565

'Pluripotent stem cells provide a powerful system to dissect the underlying molecular dynamics that regulate cell fate changes during mammalian development. Here we report the integrative analysis of genome-wide binding data for 38 transcription factors with extensive epigenome and transcriptional data across the differentiation of human embryonic stem cells to ... More

View all TaqMan™ hPSC Scorecard™ 检测板，快速 96 孔 citations