TaqMan™ hPSC Scorecard™ 检测板，快速 96 孔

You can load samples using a single channel pipette, but this method is time-consuming and may increase pipetting error. We strongly recommend that you use an 8- or 16-channel multichannel pipette. You also can dispense samples using automated systems, with the understanding that additional sample will be required to compensate for the dead volume.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center

Setting up your cDNA synthesis in 8 wells of a 96-well plate or in 8-well PCR strips facilitates sample loading of the 96-well and 384-well hPSC Scorecard Panel plates.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center

Somatic non-pluripotent primary cells, such as the parental lines used for iPSC generation, are not pluripotent in nature and their scores will be low. However, the expression of lineage markers will largely rely on homogeneity of the cells. Note that the markers in the panel are designed to evaluate early germ layer specification and not any particular terminally differentiated state.

Minor differences in gene expression profiles are sometimes observed based on the reprogramming method, but in general the hPSC Scorecard analysis results do not change significantly for lines derived using various reprogramming methods. This was tested with ESC and iPS derived using episomal or Sendai-based reprogramming systems before and after seven days of spontaneous differentiation. Cells were grown in KSR-based media on irradiated MEFs prior to removal of FGF for EB formation.

The presence or absence of Sendai virus in established iPSC clones does not have an impact on pluripotency and hence on TaqMan hPSC Scorecard analysis results.