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Thermo Scientific™
PolarScreen™ ER β 竞争剂检测试剂盒，红色
The PolarScreen™ ER Beta Competitor Assay Kit, Red, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of了解更多信息
| 货号 | 数量 |
|---|---|
| A15891 | 1 Kit |
货号 A15891
价格（CNY)
26,796.00
Each
数量:
1 Kit
价格（CNY)
26,796.00
Each
The PolarScreen™ ER Beta Competitor Assay Kit, Red, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential estrogen receptor (ER) beta ligands. The kit uses insect cell-expressed, full-length, native (untagged) human ER beta (Cat. No. A15664) and a novel, high-affinity, fluorescent estrogen ligand (Fluormone™ ES2) in a homogenous, mix-and-read assay format.
This kit produces a red color read-out; a 'green' kit is also available (Cat. No. A15890). Both kits suitably determine the IC50s of competitive compounds. Color choice depends on the spectral properties of the compounds to be screened and the available instrumentation. For additional information or assistance email drugdiscoverytech@lifetech.com.
The PolarScreen™ ER Beta Competitor Assay Kit is:
- Complete & ready-to-use out of the box—all reagents included, just add your test compounds
- High throughput compatible—20 μL fluorescence polarization assays in a 384-well plate
- Convenient—available in bulk sizes for larger screening needs
How the Assay Works
Full-length ER beta is added to a fluorescent estrogen ligand to form an ER-Fluormone™ ES2 complex. This complex is added to individual test compounds in multiwell plates. To determine relative affinity, the assay measures fluorescence polarization. Competing test compounds will displace the Fluormone™ ES2 ligand from ER beta, permitting it to tumble rapidly and resulting in low polarization values. A shift in the fluorescence polarization value in the presence of test compound is used to determine the relative affinity of test compounds for ER beta.
All components are manufactured under the strictest quality parameters. This product's performance, per the Certificate of Analysis, is guaranteed for 6 months after purchase. Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.
仅供科研使用。不可用于诊断程序。
规格
检测类别生化检测
颜色红色
成药靶标核受体
适用于（应用）核受体测定，荧光偏振
配体ER β
包装384 孔板
产品类型检测试剂盒
数量1 Kit
读值荧光偏振
靶标分子雌激素受体 β 核受体
产品线PolarScreen™
Unit SizeEach
内容与储存
• 雌激素受体 (ER) β 重组蛋白，全长（在 -68 至 -85°C 下储存）
• Fluromone™ EL 红色（在 -5 至 -30°C 下储存）
• ER 红色测定缓冲液（在室温下储存）
• Fluromone™ EL 红色（在 -5 至 -30°C 下储存）
• ER 红色测定缓冲液（在室温下储存）