PolarScreen™ 孕激素受体竞争剂检测试剂盒，绿色
PolarScreen™ 孕激素受体竞争剂检测试剂盒，绿色

PolarScreen™ 孕激素受体竞争剂检测试剂盒，绿色

PolarScreen™ 孕激素受体竞争剂检测试剂盒（绿色）为高通量荧光偏振法筛选潜在孕激素受体（PR）配体提供了灵敏高效的方法。该试剂盒采用带有谷胱甘肽-S-转移酶 (GST) 标签的昆虫细胞表达的人 PR了解更多信息
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货号数量
A15905400 x 20 μL assays
货号 A15905
价格（CNY)
15,820.00
Each
添加至购物车
数量:
400 x 20 μL assays
价格（CNY)
15,820.00
Each
添加至购物车
PolarScreen™ 孕激素受体竞争剂检测试剂盒（绿色）为高通量荧光偏振法筛选潜在孕激素受体（PR）配体提供了灵敏高效的方法。该试剂盒采用带有谷胱甘肽-S-转移酶 (GST) 标签的昆虫细胞表达的人 PR 配体结合结构域（也可单独购买），以及均一混合读数检测形式的新型高亲和力荧光孕激素受体配体 (Fluormone™ PL Green)。

该试剂盒产生绿色读数；也有“红色”试剂盒可供选择。 两种试剂盒均适合测定竞争性化合物的 IC50。颜色选择取决于待筛选化合物的光谱特性和可用的仪器。如需更多信息或帮助，请通过 drugdiscoverytech@lifetech.com 联系我们。

PolarScreen 孕激素受体竞争剂检测试剂盒为：

•全套、即开即用—包含所有试剂，只需添加测试化合物即可
•适合高通量—可以用384孔板进行荧光偏振检测
•方便—可提供大规格的组分，以满足更大的筛选需求

检测原理
在微孔板中存在竞争性测试化合物的情况下，将谷胱甘肽转移酶与人孕激素受体配体结合结构域的N末端融合体 (PR-LBD(GST)) 添加到荧光标签孕激素配体 (Fluormone PL Green) 中。有效竞争剂的存在会阻止 PL Green/PR-LBD(GST) 复合物的形成，从而降低偏振度。测试化合物存在情况下的偏振度变化可用于测定测试化合物与 PR-LBD(GST) 的相对亲和力。

如需大包装规格或帮助满足从检测方法开发到文库筛选等其他核受体检测需求，请联系我们。
仅供科研使用。不可用于诊断程序。
规格
适用于（应用）竞争性结合检测，核受体检测
配体PR
检测数量400 x 20 μL assays
产品类型检测试剂盒
数量400 x 20 μL assays
产品线PolarScreen™
Unit SizeEach
内容与储存
1管孕激素受体配体结合结构域重组蛋白（-68至 -85°C 储存）
1管 Fluormone PL Green（-5至 -30°C 储存）
2瓶 PR 筛选缓冲液（-5至 -30°C 储存）
1管1M DTT（-5至 -30°C 储存）

常见问题解答 (FAQ)

What are the common issues encountered with FP assays?

-No assay window: Check that your instrument is designed to do FP experiments.
-Poor discrimination between the minimum and maximum mP Control (both include the receptor): This may be an indication of the quality of the receptor. Do not make single use aliquots of the receptor or store diluted.
-The type of microplate is critical: Please see if the protocol specifies untreated polystyrene or NBS-coated plates. If the plate binds the fluorescent substrate, it cannot freely rotate and the mP reading will always be high. White plates cannot be used for FP assays. Avoid the use of silanized pipette tips and vials.
-The “No Receptor Control' (free Fluormone/Tracer) should have much higher RFU values compared to buffer alone. If not, there is a problem with the instrument setup or less commonly with the Fluormone/Tracer.
-If the free Fluormone/Tracer mP value is greater than 100 mP or negative, please refer to the user manual for your instrument and reset the G factor.

Find additional tips, troubleshooting help, and resources within our Drug Discovery & Development Support Center.

What is the best type of microplate reader for fluorescence polarization (FP) assays?

For FP assays, plate readers must specifically have FP built in. Please see our instrument compatibility portal (http://www.thermofisher.com/us/en/home/industrial/pharma-biopharma/drug-discovery-development/dd-misc/instrument-compatibility-portal.html).

Find additional tips, troubleshooting help, and resources within our Drug Discovery & Development Support Center.

How can I test the fluorescence polarization (FP) settings of my microplate reader before doing the assay?

We offer a FP One-Step Reference Kit (https://www.thermofisher.com/order/catalog/product/P3088) to detect in green (Ex/Em = 488/535 nm) and red (Ex/Em = 525/590 nm) emission ranges, suitable for the PolarScreen Green and Red Assays. The alternative is to perform the Controls in the respective assay kit prior to performing the actual assay using test compounds.

Find additional tips, troubleshooting help, and resources within our Drug Discovery & Development Support Center.