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Thermo Scientific™
PolarScreen™ 孕酮受体竞争剂检测试剂盒，红色
The PolarScreen™ Progesterone Receptor Competitor Assay Kit, Red, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of了解更多信息
| 货号 | 数量 |
|---|---|
| A15906 | 400 x 20 μL assays |
货号 A15906
价格（CNY)
15,664.00
Each
数量:
400 x 20 μL assays
价格（CNY)
15,664.00
Each
The PolarScreen™ Progesterone Receptor Competitor Assay Kit, Red, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential progesterone receptor (PR) ligands. The kit uses insect cell-expressed, human progesterone receptor ligand binding domain tagged with glutathione-S-transferase (GST) (also available separately) and a novel, high-affinity, fluorescent progesterone ligand (Fluormone™ PL Red) in a homogenous, mix-and-read assay format.
This kit produces a red color read-out; a 'green' kit is also available. Both kits suitably determine the IC50s of competitive compounds. Color choice depends on the spectral properties of the compounds to be screened and the available instrumentation. For additional information or assistance contact us at drugdiscoverytech@lifetech.com.
The PolarScreen Progesterone Receptor Competitor Assay is:
- Complete & ready-to-use out of the box—all reagents included, just add your test compounds
- High throughput compatible—fluorescence polarization assays in a 384-well plate
- Convenient—components available in bulk sizes for larger screening needs
How the Assay Works
An N-terminal fusion of glutathione transferase to the ligand-binding domain of the human progesterone receptor (PR-LBD(GST)) is added to a fluorescently-tagged progesterone ligand (Fluormone PL Red) in the presence of competitor test compounds in microwell plates. The presence of effective competitors prevents the formation of a PL Red/PR-LBD(GST) complex resulting in a decrease of the polarization value. The shift in polarization value in the presence of test compounds is used to determine relative affinity of test compounds for PR-LBD(GST).
Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.
仅供科研使用。不可用于诊断程序。
规格
适用于（应用）竞争性结合检测，核受体检测
配体PR
检测数量400 x 20 μL Assays
产品类型检测试剂盒
数量400 x 20 μL assays
产品线PolarScreen™
Unit SizeEach
内容与储存
1 管孕酮受体配体结合结构域重组蛋白（在 -68 至 -85°C 下储存）
1 管 Fluormone PL Red 示踪剂（在 -5 至 -30°C 下储存）
2 瓶 PR Red 筛选缓冲液（在 -5 至 -30°C 下储存）
1 管 DTT（在 -5 至 -30°C 下储存）
1 管 Fluormone PL Red 示踪剂（在 -5 至 -30°C 下储存）
2 瓶 PR Red 筛选缓冲液（在 -5 至 -30°C 下储存）
1 管 DTT（在 -5 至 -30°C 下储存）