Epi5™ Episomal iPSC 重编程试剂盒
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Epi5™ Episomal iPSC 重编程试剂盒
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Epi5™ Episomal iPSC 重编程试剂盒

Epi5™ Episomal iPSC 重编程试剂盒提供一组 5 个由 Okita 博士在京都大学 iPS 细胞研究及应用中心了解更多信息
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货号数量
A159601 kit
货号 A15960
价格(CNY)
17,766.40
飞享价
Ends: 31-Dec-2025
22,519.00
共减 4,752.60 (21%)
Each
添加至购物车
数量:
1 kit
价格(CNY)
17,766.40
飞享价
Ends: 31-Dec-2025
22,519.00
共减 4,752.60 (21%)
Each
添加至购物车
Epi5™ Episomal iPSC 重编程试剂盒提供一组 5 个由 Okita 博士在京都大学 iPS 细胞研究及应用中心 (CiRA) 的 Yamanaka 教授实验室中进行设计的易于使用、高效游离型载体。本系统可产生无转基因、无病毒人诱导多能干细胞 (iPSC),在 0.04% 至 0.3 范围内其效率取决于重编程的细胞类型。

Epi5™ Episomal iPSC 重编程试剂盒的特点包括:

• 易于使用且高效—在重编程过程中无需使用小分子,并且已添加的 p53 抑制可提高 iPSC 生成 (1)
• 可用于您的 iPSC 研究的所有阶段—无转基因且无病毒的重编程使得操作可用于从基础研究到临床前研究
• 培养基系统具有灵活性—可用于无饲养层或基于饲养层的培养基系统

创建转基因和无病毒 iPSC
游离型载体是一种经过充分描述的用于生成无转基因、无病毒 iPSC 的系统,为多能干细胞研究的所有阶段提供 iPSC 来源。其他重编程方法,例如慢病毒属,包含可整合到宿主基因组的转基因,可能破坏宿主基因组,或者引起其它不可预测的结果。

作为 oriP/EBNA1 载体,这些游离型载体包含 5 种重编程因子(Oct4、Sox2、Lin28、Klf4 和 L-Myc),并在每个细胞周期中在染色体外仅复制一次。在这种复制速率下,以每细胞代约 5% 的速率丢失游离体。该系统通过 p53 抑制增强 iPSC 生成,并证明人类 iPSC 生成期间包含的 L-Myc 的效力和特异性均高于 c-Myc (1)。

系统易于使用
该 2 样品瓶套件可为游离型载体重编程提供较佳系统,无需在重编程培养基中使用小分子。游离型载体通过电穿孔引入细胞。我们推荐使用 Neon™ 转染系统进行电穿孔,以便灵活改变体积,从而改变重编程所需的细胞数量。

可灵活选择培养基系统
Epi5™ Episomal iPSC 重编程试剂盒可与多种细胞类型和培养基系统配合使用。成纤维细胞和 CD34+ 血细胞的方案可以提供饲养层或无饲养层的重编程。

1.Okita K、Matsumara Y、Sato Y 等人。生成无整合人 iPS 细胞的更有效方法。Nature Methods 8, 409-412, 2011.
仅供科研使用。不可用于诊断程序。
规格
克隆方法
输送类型转染
适用于(应用)干细胞工程和重编程
适用于(设备)电转化仪、Neon 转染系统
关键功能el
产品线Epi5™
产品类型Episomal iPSC 重编程试剂盒
促进剂CAG
蛋白标记
数量1 kit
复制起点pUC 复制区序列
报告基因
选择试剂(真核生物)
载体EBNA OriP
Unit SizeEach
内容与储存
2 管。在 -5 至 -20°C 下储存。

常见问题解答 (FAQ)

你们提供何种用于干细胞重编程的试剂盒?

我们提供Invitrogen CytoTune-iPS 2.0仙台病毒重编程试剂盒(Invitrogen CytoTune-iPS 2.0 Sendai Reprogramming Kit,货号A16517,A16518),这是一套非整合型的系统,通过仙台病毒载体将体细胞重编程为诱导性多能干细胞(iPSCs)。Invitrogen CytoTune-iPS 2.0仙台病毒重编程试剂盒包含了三种用于递送和表达关键遗传因子的Invitrogen CytoTune 2.0重编程载体,这些因子的表达对于将体细胞重编程为iPSCs的操作是必需的。只需要一次应用这些载体即可成功的实现重编程。

此外,我们还提供附着体iPSC重编程载体 (货号A14703) 或 Epi5附着体iPSC重编程试剂盒 (货号 A15960)。其同样是非整合型系统能将体细胞重编程为诱导性多能干细胞(iPSCs)。本品是六种或五种载体的混合物,为在无饲养层环境下生成iPSC提供优化的体系,具有非病毒介导、无基因组整合的特点。这些附着体iPSC重编程载体最初由Junying Yu与James Thomson开发(http://www.ncbi.nlm.nih.gov/pubmed/19325077),并经过Cellular Dynamics International的进一步优化,能够胜任大量不同种类的体细胞的重编程操作。

Can I use pen/strep or other antibiotics for episomal reprogramming of cells?

The use of antibiotics is not recommended during episomal reprogramming, especially Fungizone antimycotic. If antibiotics must be used during the reprogramming process, pen/strep could be used. For established iPSCs (after reprogramming is complete), either antibiotic/antimycotic or pen/strep should be fine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I reprogram mouse cells with the Epi5 Episomal iPSC Reprograming Kit (Cat. No. A15960)?

No. The Epi5 Episomal iPSC Reprogramming Kit (Cat. No. A15960) was designed to reprogram human cells, not mouse cells. We currently only have data with this kit on reprogramming human cells, which aligns with the use of EBNA/oriP episomal vectors mainly in primate cells. Although rodent cells lack the oriP replicon and are not permissive, some studies replaced or modified oriP with other sequences to mediate replication and some literature reported the use of unmodified EBNA/oriP in certain hamster and mouse lines.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

When generating iPSCs using the Epi5 Episomal iPSC Reprogramming Kit (Cat. No. A15960), how confluent should my cells be on the day of transfection?

When generating iPSCs using the Epi5 Episomal iPSC Reprogramming Kit (Cat. No. A15960) cells should be approximately 75-90% confluent on the day of transfection (Day 0). Since over-confluency results in decreased transfection efficiency, we recommend replating overly confluent cells to achieve 75-90% confluency on the day of transfection.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How many reprogramming experiments can I perform with the Epi5 Episomal iPSC Reprogramming Kit (Cat. No. A15960)?

The Epi5 Episomal iPSC Reprogramming Kit (Cat. No. A15960) provides enough material to process 20 reprogramming experiments (each reprogramming experiment requires 1 × 105 cells and 1 µL each of the Epi5 Reprogramming Vectors and the Epi5 p53 & EBNA Vectors). Each vial of vectors in the kit contains 20 µL, and you will use 1 µL (from each vial) per experiment. Before use, briefly centrifuge the thawed vectors to collect them at the bottom of the tube.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.