B-27™ Supplement, minus insulin
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B-27™ Supplement, minus insulin
Gibco™

B-27™ Supplement, minus insulin

A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Insulin is a customized version of theRead more
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Catalog NumberQuantity
A189560110 mL
A1895602100 mL
Catalog number A1895601
Price (CNY)
1,477.21
Online Exclusive
Ends: 31-Dec-2025
2,208.00
Save 730.79 (33%)
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Quantity:
10 mL
Price (CNY)
1,477.21
Online Exclusive
Ends: 31-Dec-2025
2,208.00
Save 730.79 (33%)
Each
Add to cart
A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Insulin is a customized version of the original B-27 Supplement without insulin. B-27 Supplement Minus Insulin is a serum-free supplement designed to support growth of hippocampal and other CNS neurons in applications where the presence of insulin causes interference. B-27 Supplement Minus Insulin is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium.

B-27 Supplement is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of B-27 Supplement and Neurobasal media.

For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeNeural Cells
Concentration50 X
Culture TypeMammalian Cell Culture
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineB-27™
Quantity10 mL
Serum LevelSerum-Free
Shelf Life12 Months
Shipping ConditionDry Ice
SourceSerum-Free
Tested ForEndotoxin, Performance, Sterility, Mycoplasma
ClassificationSerum-free
FormLiquid
Product TypeCell Culture Supplement
SterilitySterile-filtered
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -20°C). Protect from light.

Frequently asked questions (FAQs)

Do you offer B-27 supplements with and without insulin?

Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I thaw B-27 Supplements in a 37 degrees C water bath or incubator?

We do not recommend thawing at 37 degrees C. Best results are observed upon thawing at room temperature for approximately 2 hrs or overnight at 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I thaw B-27 Supplements?

We recommend thawing it at 4 degrees C overnight or at room temperature for 2 hours. We don't recommend thawing in a water bath or incubator.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I re-freeze B-27 Supplements into smaller aliquots for future use?

Yes, we recommend that you aliquot when you first thaw it and thereafter subject the aliquot to no more than 2 additional freeze/thaw cycles.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.