FluoroBrite™ DMEM
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FluoroBrite™ DMEM
Citations & References (21)
Gibco™

FluoroBrite™ DMEM

Gibco™ FluoroBrite™ DMEM features a background fluorescence that is comparable to PBS and 90% lower than that emitted by standard phenol red–free DMEM.
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Catalog NumberQuantity
A1896701500 mL
A189670210 x 500 mL
Catalog number A1896701
Price (CNY)
675.20
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Ends: 31-Dec-2025
861.00
Save 185.80 (22%)
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Quantity:
500 mL
Price (CNY)
675.20
Online Exclusive
Ends: 31-Dec-2025
861.00
Save 185.80 (22%)
Each
Add to cart

Gibco™ FluoroBrite™ DMEM features a background fluorescence that is comparable to PBS and 90% lower than that emitted by standard phenol red–free DMEM. Formulated to include the required nutrients for routine cell culture when supplemented with 10% fetal bovine serum and 4 mM L-glutamine or GlutaMAX™ Supplement, FluoroBrite™ DMEM is designed to enhance the signal-to-noise ratio of fluorophores, enabling researchers to visualize even the weakest fluorescent events in an environment that promotes optimum cell health. Additional features include:

  • Enhancement of fluorescence signal during live-cell imaging
  • DMEM-based to help preserve cell health

Live-cell fluorescence microscopy is an essential technique for the visualization of fundamentally important and physiologically relevant biological events. A key challenge with this technique is the ability to image weak fluorophores without causing cell damage, photobleaching, or undesirable changes to cell health. FluoroBrite™ DMEM helps address these issues.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product TypeDMEM (Dulbecco's Modified Eagle Medium)
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
With AdditivesHigh Glucose
Without AdditivesNo Glutamine, No HEPES, No Phenol Red, No Sodium Pyruvate
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C). Protect from light.

Frequently asked questions (FAQs)

What is the osmolality of Fluorobrite DMEM?

We do provide osmolality information on the certificate of analysis. All lots of Fluorobrite DMEM (Cat. Nos. A1896701 and A1896702) will meet the osmolality specification of 320-350 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (21)

Citations & References
Abstract
Open source software for quantification of cell migration, protrusions, and fluorescence intensities.
Authors:Barry DJ, Durkin CH, Abella JV, Way M,
Journal:
PubMed ID:25847537
'Cell migration is frequently accompanied by changes in cell morphology (morphodynamics) on a range of spatial and temporal scales. Despite recent advances in imaging techniques, the application of unbiased computational image analysis methods for morphodynamic quantification is rare. For example, manual analysis using kymographs is still commonplace, often caused by ... More
A BRCA1-interacting lncRNA regulates homologous recombination.
Authors:Sharma V, Khurana S, Kubben N, Abdelmohsen K, Oberdoerffer P, Gorospe M, Misteli T,
Journal:
PubMed ID:26412854
Long non-coding RNAs (lncRNAs) are important players in diverse biological processes. Upon DNA damage, cells activate a complex signaling cascade referred to as the DNA damage response (DDR). Using a microarray screen, we identify here a novel lncRNA, DDSR1 (DNA damage-sensitive RNA1), which is induced upon DNA damage. DDSR1 induction ... More
Ebola Virus and Severe Acute Respiratory Syndrome Coronavirus Display Late Cell Entry Kinetics: Evidence that Transport to NPC1+ Endolysosomes Is a Rate-Defining Step.
Authors:Mingo RM, Simmons JA, Shoemaker CJ, Nelson EA, Schornberg KL, D'Souza RS, Casanova JE, White JM,
Journal:
PubMed ID:25552710
Ebola virus (EBOV) causes hemorrhagic fevers with high mortality rates. During cellular entry, the virus is internalized by macropinocytosis and trafficked through endosomes until fusion between the viral and an endosomal membrane is triggered, releasing the RNA genome into the cytoplasm. We found that while macropinocytotic uptake of filamentous EBOV ... More
3D imaging of Sox2 enhancer clusters in embryonic stem cells.
Authors:Liu Z, Legant WR, Chen BC, Li L, Grimm JB, Lavis LD, Betzig E, Tjian R,
Journal:
PubMed ID:25537195
Combinatorial cis-regulatory networks encoded in animal genomes represent the foundational gene expression mechanism for directing cell-fate commitment and maintenance of cell identity by transcription factors (TFs). However, the 3D spatial organization of cis-elements and how such sub-nuclear structures influence TF activity remain poorly understood. Here, we combine lattice light-sheet imaging, ... More
Microtubule-dependent transport and dynamics of vimentin intermediate filaments.
Authors:Hookway C, Ding L, Davidson MW, Rappoport JZ, Danuser G, Gelfand VI,
Journal:
PubMed ID:25717187
We studied two aspects of vimentin intermediate filament dynamics-transport of filaments and subunit exchange. We observed transport of long filaments in the periphery of cells using live-cell structured illumination microscopy. We studied filament transport elsewhere in cells using a photoconvertible-vimentin probe and total internal reflection microscopy. We found that filaments ... More
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