7-氨基-4-甲基香豆素，标准物质
7-氨基-4-甲基香豆素，标准物质
引用和文献 (11)
Invitrogen™

7-氨基-4-甲基香豆素，标准物质

7-Amino-4-methylcoumarin (AMC) can be used as a fluorescence reference standard for AMC-based enzyme substrates including AMC-based caspase substrates. The fluorescence了解更多信息
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货号数量
A191100 mg
货号 A191
价格（CNY)
2,303.00
Each
添加至购物车
数量:
100 mg
价格（CNY)
2,303.00
Each
添加至购物车

7-Amino-4-methylcoumarin (AMC) can be used as a fluorescence reference standard for AMC-based enzyme substrates including AMC-based caspase substrates. The fluorescence spectra of AMC is not subject to variability due to pH-dependent protonation/deprotonation when assayed near or above physiological pH.

To make a concentrated stock solution of up to 10 mg/mL, dissolve in either acetone, high quality anhydrous DMSO or DMF. Store frozen, desiccated and protected from light.

仅供科研使用。不可用于诊断程序。
规格
发射430 nm
激发351 nm
标签或染料甲基香豆素
分子量175.2
产品类型7-氨基-4-甲基香豆素
数量100 mg
运输条件室温
标签类型香豆素染料
Unit SizeEach
内容与储存
室温避光储存。

常见问题解答 (FAQ)

What solvent should I use to reconstitute 7-Amino-4-Methylcoumarin, reference standard (Cat. No. A191)? Once reconstituted, can the product be aliquoted and stored at -20 degrees C without damaging the molecule?

The 7-Amino-4-Methylcoumarin, reference standard (Cat. No. A191) can be resuspended in DMF or DMSO at a concentration of at least 1 mM. The molecular weight is 175.19 g/mol.
Yes, the solution can be stored in aliquots at -20 degrees C for at least 6 months.

Find additional tips, troubleshooting help, and resources within our Protein Standards and Ladders Support Center.

引用和文献 (11)

引用和文献
Abstract
High-performance liquid affinity chromatography and in situ fluorescent labeling on thin-layer chromatography of glycosphingolipids.
Authors:Tomono Y, Abe K, Watanabe K
Journal:Anal Biochem
PubMed ID:2327579
'A method combining high-performance liquid affinity chromatography and in situ fluorescent labeling on thin-layer chromatography is introduced for determination of glycosphingolipids. Glycolipids in crude extract from rat liver were separated quantitatively from neutral lipids and phospholipids with a phenylboronic acid-derivatized silica gel column. Glycolipids were eluted quantitatively with approximately 98% ... More
Visual detection of peptidase activity using fluorogenic substrates in a microtiter plate assay.
Authors:Irvine GB, Ennis M, Williams CH
Journal:Anal Biochem
PubMed ID:2339787
'A simple, inexpensive, and sensitive assay for peptidase activity has been devised. The assay was performed in a microtiter plate and was based on fluorogenic peptide substrates, many of which are commercially available. 7-Amino-4-methyl coumarin the fluorescent product liberated during an incubation period of between 1 and 16 h, was ... More
New fluorogenic substrates for subtilisin.
Authors:Kanaoka Y, Takahashi T, Nakayama H, Tanizawa K
Journal:Chem Pharm Bull (Tokyo)
PubMed ID:3899387
A new fluorescent tag for labeling of saccharides.
Authors:Prakash C, Vijay IK
Journal:Anal Biochem
PubMed ID:6846798
Potential aldehyde groups of N-acetylglucosamine and its di- and tri-saccharides were coupled with 7-amino-4-methylcoumarin (AMC) by reductive amination in the presence of sodium cyanoborohydride. The products were purified by silica gel chromatography. Acid hydrolysis and digestion with glycosidases gave the expected products. One picomole of the sugar-AMC complexes could be ... More
A new fluorogenic substrate for plasmin.
Authors:Pierzchala PA, Dorn CP, Zimmerman M
Journal:Biochem J
PubMed ID:161507
A new fluorogenic peptide substrate for plasmin, 7-(N-succinoylalanylphenylalanyl-lysylamido)-4-methylcoumarin trifluoroacetate salt, was prepared that can be used in a simple and direct assay. The results obtained by the assay method are linear over a wide range of enzyme concentrations and sensitive enough to detect as little as 10(-5) CTA units of ... More
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