Segregation of nitrogen fixation and oxygenic photosynthesis in the marine cyanobacterium Trichodesmium.
AuthorsBerman-Frank I, Lundgren P, Chen YB, Küpper H, Kolber Z, Bergman B, Falkowski P
JournalScience
PubMed ID11711677
'In the modern ocean, a significant amount of nitrogen fixation is attributed to filamentous, nonheterocystous cyanobacteria of the genus Trichodesmium. In these organisms, nitrogen fixation is confined to the photoperiod and occurs simultaneously with oxygenic photosynthesis. Nitrogenase, the enzyme responsible for biological N2 fixation, is irreversibly inhibited by oxygen in ... More
Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates.
AuthorsPanchuk-Voloshina N, Haugland RP, Bishop-Stewart J, Bhalgat MK, Millard PJ, Mao F, Leung WY, Haugland RP
JournalJ Histochem Cytochem
PubMed ID10449539
'Alexa 350, Alexa 430, Alexa 488, Alexa 532, Alexa 546, Alexa 568, and Alexa 594 dyes are a new series of fluorescent dyes with emission/excitation spectra similar to those of AMCA, Lucifer Yellow, fluorescein, rhodamine 6G, tetramethylrhodamine or Cy3, lissamine rhodamine B, and Texas Red, respectively (the numbers in the ... More
High density lipoprotein (HDL) particle uptake mediated by scavenger receptor class B type 1 results in selective sorting of HDL cholesterol from protein and polarized cholesterol secretion.
AuthorsSilver DL, Wang N, Xiao X, Tall AR
JournalJ Biol Chem
PubMed ID11301333
'High density lipoprotein (HDL) mediates reverse transport of cholesterol from atheroma foam cells to the liver, but the mechanisms of hepatic uptake and trafficking of HDL particles are poorly understood. In contrast to its accepted role as a cell surface receptor, scavenger receptor class B type 1 (SR-BI) is shown ... More
Differential requirement for classic and novel PKC isoforms in respiratory burst and phagocytosis in RAW 264.7 cells.
AuthorsLarsen EC, DiGennaro JA, Saito N, Mehta S, Loegering DJ, Mazurkiewicz JE, Lennartz MR
JournalJ Immunol
PubMed ID10946313
'The binding of Ab (IgG)-opsonized particles by FcgammaRs on macrophages results in phagocytosis of the particles and generation of a respiratory burst. Both IgG-stimulated phagocytosis and respiratory burst involve activation of protein kinase C (PKC). However, the specific PKC isoforms required for these responses have yet to be identified. We ... More
Acid-induced conformational changes in phosphoglucose isomerase result in its increased cell surface association and deposition on fibronectin fibrils.
AuthorsAmraei M, Jia Z, Reboul P, Nabi IR
JournalJ Biol Chem
PubMed ID12888574
'Phosphoglucose isomerase (PGI) is a glycolytic enzyme that exhibits extracellular cytokine activity as autocrine motility factor, neuroleukin, and maturation factor and that has been recently implicated as an autoantigen in rheumatoid arthritis. In contrast to its receptor-mediated endocytosis at neutral pH, addition of 25 microg/ml of either Alexa 568- or ... More
Serum amyloid A is a ligand for scavenger receptor class B type I and inhibits high density lipoprotein binding and selective lipid uptake.
AuthorsCai L, de Beer MC, de Beer FC, van der Westhuyzen DR
JournalJ Biol Chem
PubMed ID15561721
'Serum amyloid A is an acute phase protein that is carried in the plasma largely as an apolipoprotein of high density lipoprotein (HDL). In this study we investigated whether SAA is a ligand for the HDL receptor, scavenger receptor class B type I (SR-BI), and how SAA may influence SR-BI-mediated ... More
Protein interacting with C kinase 1 (PICK1) reduces reinsertion rates of interaction partners sorted to Rab11-dependent slow recycling pathway.
AuthorsMadsen KL, Thorsen TS, Rahbek-Clemmensen T, Eriksen J, Gether U,
JournalJ Biol Chem
PubMed ID22303009
'The scaffolding protein PICK1 (protein interacting with C kinase 1) contains an N-terminal PSD-95/Discs large/ZO-1 (PDZ) domain and a central lipid-binding Bin/amphiphysin/Rvs (BAR) domain. PICK1 is thought to regulate trafficking of its PDZ binding partners but different and even opposing functions have been suggested. Here, we apply ELISA-based assays and ... More
Differences in endocytosis and intracellular sorting of ricin and viscumin in 3T3 cells.
AuthorsMoisenovic M, Tonevitsky A, Agapov I, Niwa H, Schewe H, Bereiter-Hahn J
JournalEur J Cell Biol
PubMed ID12437187
'Ricin and viscumin are heterodimeric protein toxins. Their A-chain is enzymatically active and removes an adenine residue from the 28S rRNA, the B-chain has lectin activity and binds to terminal galactose residues of cell surface receptors. The toxins reveal a high degree of identity in their amino acid sequences. Nevertheless, ... More
Redundant mechanisms recruit actin into the contractile ring in silkworm spermatocytes.
AuthorsChen W, Foss M, Tseng KF, Zhang D,
JournalPLoS Biol
PubMed ID18767903
'Cytokinesis is powered by the contraction of actomyosin filaments within the newly assembled contractile ring. Microtubules are a spindle component that is essential for the induction of cytokinesis. This induction could use central spindle and/or astral microtubules to stimulate cortical contraction around the spindle equator (equatorial stimulation). Alternatively, or in ... More
ECM degradation assays for analyzing local cell invasion.
AuthorsArtym VV, Yamada KM, Mueller SC,
JournalMethods Mol Biol
PubMed ID19247615
'Proteolytic degradation of extracellular matrix (ECM) is a critical step during cell invasion and tissue transmigration that is required for many physiological and pathological processes. Cellular structures that mediate cell adhesion to, degradation of, and invasion into ECM are invadopodia of transformed and tumor cells and podosomes of normal monocytic, ... More
Activation-induced deaminase cloning, localization, and protein extraction from young VH-mutant rabbit appendix.
'Studies in mouse, human, and chicken suggest that activation-induced deaminase (AID) is involved in three known processes leading to antibody diversification: somatic hypermutation, gene conversion, and class-switch recombination. Developing rabbit appendix provides a particularly good site for studying all three of these B cell maturation events. We report here successful ... More
Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging.
AuthorsDempsey GT, Vaughan JC, Chen KH, Bates M, Zhuang X,
JournalNat Methods
PubMed ID22056676
'One approach to super-resolution fluorescence imaging uses sequential activation and localization of individual fluorophores to achieve high spatial resolution. Essential to this technique is the choice of fluorescent probes; the properties of the probes, including photons per switching event, on-off duty cycle, photostability and number of switching cycles, largely dictate ... More
H-type dimer formation of fluorophores: a mechanism for activatable, in vivo optical molecular imaging.
AuthorsOgawa M, Kosaka N, Choyke PL, Kobayashi H,
JournalACS Chem Biol
PubMed ID19480464
'In vivo molecular imaging with target-specific activatable '
Controlling the rates of biochemical reactions and signaling networks by shape and volume changes.
AuthorsLizana L, Bauer B, Orwar O,
JournalProc Natl Acad Sci U S A
PubMed ID18337513
'In biological systems, chemical activity takes place in micrometer- and nanometer-sized compartments that constantly change in shape and volume. These ever-changing cellular compartments embed chemical reactions, and we demonstrate that the rates of such incorporated reactions are directly affected by the ongoing shape reconfigurations. First, we show that the rate ... More
Redirecting lipoic acid ligase for cell surface protein labeling with small-molecule probes.
'Live cell imaging is a powerful method to study protein dynamics at the cell surface, but conventional imaging probes are bulky, or interfere with protein function, or dissociate from proteins after internalization. Here, we report technology for covalent, specific tagging of cellular proteins with chemical probes. Through rational design, we ... More
AuthorsSabirsh A, Wetterholm A, Bristulf J, Leffler H, Haeggström JZ, Owman C
JournalJ Lipid Res
PubMed ID15805550
'Leukotriene B4 (LTB4) is a potent lipid mediator of inflammation that acts primarily via a seven-transmembrane-spanning, G-protein-coupled receptor denoted BLT1. Here, we describe the synthesis and characterization of fluorescent analogs of LTB4 that are easy to produce, inexpensive, and without the disadvantages of a radioligand. Fluorescent LTB4 is useful for ... More
The human natural killer cell immune synapse.
AuthorsDavis DM, Chiu I, Fassett M, Cohen GB, Mandelboim O, Strominger JL
JournalProc Natl Acad Sci U S A
PubMed ID10611338
'Inhibitory killer Ig-like receptors (KIR) at the surface of natural killer (NK) cells induced clustering of HLA-C at the contacting surface of target cells. In this manner, inhibitory immune synapses were formed as human NK cells surveyed target cells. At target/NK cell synapses, HLA-C/KIR distributed into rings around central patches ... More
Multicolor imaging: the important question of co-localization.
AuthorsSmallcombe A
JournalBiotechniques
PubMed ID11414212
Controlling enzymatic reactions by geometry in a biomimetic nanoscale network.
AuthorsSott K, Lobovkina T, Lizana L, Tokarz M, Bauer B, Konkoli Z, Orwar O
JournalNano Lett
PubMed ID16464036
We demonstrate that a transition from a compact geometry (sphere) to a structured geometry (several spheres connected by nanoconduits) in nanotube-vesicle networks (NVNs) induces an ordinary enzyme-catalyzed reaction to display wavelike properties. The reaction dynamics can be controlled directly by the geometry of the network, and such networks can be ... More
Electrochemical release of fluorescently labeled thiols from patterned gold surfaces.
AuthorsGhaly T, Wildt BE, Searson PC,
JournalLangmuir
PubMed ID19902935
Reductive desorption of alkanethiols is a tool for spatially and temporally controlled release of small molecules or particles from individually addressable gold electrodes. Here we report on the dynamics of release using fluorophore-terminated C6 or C11 thiols. We show that the release kinetics for C6 thiols are determined solely by ... More
A chemical method for fast and sensitive detection of DNA synthesis in vivo.
AuthorsSalic A, Mitchison TJ,
JournalProc Natl Acad Sci U S A
PubMed ID18272492
We have developed a method to detect DNA synthesis in proliferating cells, based on the incorporation of 5-ethynyl-2'-deoxyuridine (EdU) and its subsequent detection by a fluorescent azide through a Cu(I)-catalyzed [3 + 2] cycloaddition reaction ("click" chemistry). Detection of the EdU label is highly sensitive and can be accomplished in ... More
Fluorescent histochemical techniques for analysis of intracellular signaling.
AuthorsOksvold MP, Skarpen E, Widerberg J, Huitfeldt HS
JournalJ Histochem Cytochem
PubMed ID11850432
Intracellular signaling relies on the orchestrated cooperation of signaling proteins and modules, their intracellular localization, and membrane trafficking. Recently, a repertoire of fluorescence-based techniques, which significantly increases our potential for detailed studies of the involved mechanisms, has been introduced. Microscopic techniques with increased resolution have been combined with improved techniques ... More
IL-1 receptor blockade restores autophagy and reduces inflammation in chronic granulomatous disease in mice and in humans.
Authorsde Luca A, Smeekens SP, Casagrande A, Iannitti R, Conway KL, Gresnigt MS, Begun J, Plantinga TS, Joosten LA, van der Meer JW, Chamilos G, Netea MG, Xavier RJ, Dinarello CA, Romani L, van de Veerdonk FL,
Journal
PubMed ID24550444
Patients with chronic granulomatous disease (CGD) have a mutated NADPH complex resulting in defective production of reactive oxygen species; these patients can develop severe colitis and are highly susceptible to invasive fungal infection. In NADPH oxidase-deficient mice, autophagy is defective but inflammasome activation is present despite lack of reactive oxygen ... More
An in vitro assay for entry into cilia reveals unique properties of the soluble diffusion barrier.
Specific proteins are concentrated within primary cilia, whereas others remain excluded. To understand the mechanistic basis of entry into cilia, we developed an in vitro assay using cells in which the plasma membrane was permeabilized, but the ciliary membrane was left intact. Using a diffusion-to-capture system and quantitative analysis, we ... More
A cell-free system for light-dependent nuclear import of phytochrome.
AuthorsPfeiffer A, Kunkel T, Hiltbrunner A, Neuhaus G, Wolf I, Speth V, Adam E, Nagy F, Schäfer E,
JournalPlant J
PubMed ID18980642
Translocation from the cytosol to the nucleus is an essential step in phytochrome (phy) signal transduction. In the case of phytochrome A (phyA), this step occurs with the help of FHY1 (far-red-elongated hypocotyl 1), a specific transport protein. To investigate the components involved in phyA transport, we used a cell-free ... More
The association of autophagy with polyethylenimine-induced cytotoxicity in nephritic and hepatic cell lines.
AuthorsGao X, Yao L, Song Q, Zhu L, Xia Z, Xia H, Jiang X, Chen J, Chen H,
JournalBiomaterials
PubMed ID21903261
Polyethylenimine (PEI) is one of the most effective and widely used cationic macromolecules in experimental gene transfer/therapy protocols. However, the further clinical application of PEI is largely impeded by its cytotoxicity. Here we performed a fundamental investigation on the mechanism of PEI-induced cytotoxicity in both hepatic and nephritic cell lines. ... More
Structure and composition of aggregates in two large European rivers, based on confocal laser scanning microscopy and image and statistical analyses.
AuthorsLuef B, Neu TR, Zweimüller I, Peduzzi P,
JournalAppl Environ Microbiol
PubMed ID19633114
Floating riverine aggregates are composed of a complex mixture of inorganic and organic components from their respective aquatic habitats. Their architecture and integrity are supplemented by the presence of extracellular polymeric substances of microbial origin. They are also a habitat for virus-like particles, bacteria, archaea, fungi, algae, and protozoa. In ... More
Dendritic spine heterogeneity determines afferent-specific Hebbian plasticity in the amygdala.
AuthorsHumeau Y, Herry C, Kemp N, Shaban H, Fourcaudot E, Bissière S, Lüthi A
JournalNeuron
PubMed ID15629707
Functional compartmentalization of dendrites is thought to underlie afferent-specific integration of neural activity in laminar brain structures. Here we show that in the lateral nucleus of the amygdala (LA), an area lacking apparent laminar organization, thalamic and cortical afferents converge on the same dendrites, contacting neighboring but morphologically and functionally ... More
Alpha4 integrins are type I cAMP-dependent protein kinase-anchoring proteins.
AuthorsLim CJ, Han J, Yousefi N, Ma Y, Amieux PS, McKnight GS, Taylor SS, Ginsberg MH
JournalNat Cell Biol
PubMed ID17369818
A-kinase anchoring proteins (AKAPs) control the localization and substrate specificity of cAMP-dependent protein kinase (PKA), tetramers of regulatory (PKA-R) and catalytic (PKA-C) subunits, by binding to PKA-R subunits. Most mammalian AKAPs bind Type II PKA through PKA-RII (ref. 2), whereas dual specificity AKAPs bind both PKA-RI and PKA-RII (ref. 3). ... More
Dissecting RNA-interference pathway with small molecules.
AuthorsChiu YL, Dinesh CU, Chu CY, Ali A, Brown KM, Cao H, Rana TM
JournalChem Biol
PubMed ID15975509
RNA interference (RNAi) is a process whereby short-interfering RNAs (siRNA) silence gene expression in a sequence-specific manner. We have screened a chemical library of substituted dihydropteridinones and identified a nontoxic, cell permeable, and reversible inhibitor of the RNAi pathway in human cells. Biochemical and fluorescence resonance-energy transfer experiments demonstrated that ... More
Tracking sodium channels in live cells: confocal imaging using fluorescently labeled toxins.
One particularly important class of ion channels in excitable cells are the voltage-dependent sodium channels (VDSC). Knowledge of the distribution of VDSC in living cells is important for studies of neuronal excitability, development, and plasticity. Here, we demonstrate a new method for visualizing the spatial distribution of VDSC in living ... More
SPARC regulates extracellular matrix organization through its modulation of integrin-linked kinase activity.
AuthorsBarker TH, Baneyx G, Cardó-Vila M, Workman GA, Weaver M, Menon PM, Dedhar S, Rempel SA, Arap W, Pasqualini R, Vogel V, Sage EH
JournalJ Biol Chem
PubMed ID16115889
SPARC, a 32-kDa matricellular glycoprotein, mediates interactions between cells and their extracellular matrix, and targeted deletion of Sparc results in compromised extracellular matrix in mice. Fibronectin matrix provides provisional tissue scaffolding during development and wound healing and is essential for the stabilization of mature extracellular matrix. Herein, we report that ... More
Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition.
Seven-color analyses of immunofluorescence-stained tissue samples were accomplished using Fourier spectroscopy-based hyperspectral imaging and singular value decomposition. This system consists of a combination of seven fluorescent dyes, three filtersets, an epifluorescence microscope, a spectral imaging system, a computer for data acquisition, and data analysis software. The spectra of all pixels ... More
Monovalent ligation of the B cell receptor induces receptor activation but fails to promote antigen presentation.
AuthorsKim YM, Pan JY, Korbel GA, Peperzak V, Boes M, Ploegh HL
JournalProc Natl Acad Sci U S A
PubMed ID16492756
We explored the role of antigen valency in B cell receptor (BCR) activation and rearrangement of intracellular MHC class II compartments as factors that contribute to the efficacy of antigen presentation. Using primary B cells that express a hen egg lysozyme (HEL)-specific BCR, we found that oligomeric HEL more efficiently ... More
The tetraspanin CD63/lamp3 cycles between endocytic and secretory compartments in human endothelial cells.
AuthorsKobayashi T, Vischer UM, Rosnoblet C, Lebrand C, Lindsay M, Parton RG, Kruithof EK, Gruenberg J
JournalMol Biol Cell
PubMed ID10793155
In the present study, we show that in human endothelial cells the tetraspanin CD63/lamp3 distributes predominantly to the internal membranes of multivesicular-multilamellar late endosomes, which contain the unique lipid lysobisphosphatidic acid. Some CD63/lamp3 is also present in Weibel-Palade bodies, the characteristic secretory organelle of these cells. We find that CD63/lamp3 ... More
Recycling of apoprotein E is associated with cholesterol efflux and high density lipoprotein internalization.
AuthorsHeeren J, Grewal T, Laatsch A, Rottke D, Rinninger F, Enrich C, Beisiegel U
JournalJ Biol Chem
PubMed ID12584196
After receptor-mediated endocytosis of triglyceride-rich lipoproteins (TRL) into the liver, TRL particles are immediately disintegrated in peripheral endosomal compartments. Whereas core lipids and apoprotein B are delivered for degradation into lysosomes, TRL-derived apoE is efficiently recycled back to the plasma membrane. This is followed by apoE re-secretion and association of ... More
PlyC: a multimeric bacteriophage lysin.
AuthorsNelson D, Schuch R, Chahales P, Zhu S, Fischetti VA
JournalProc Natl Acad Sci U S A
PubMed ID16818874
Lysins are murein hydrolases produced by bacteriophage that act on the bacterial host cell wall to release progeny phage. When added extrinsically in their purified form, these enzymes produce total lysis of susceptible Gram-positive bacteria within seconds, suggesting a unique antimicrobial strategy. All known Gram-positive lysins are produced as a ... More
High density lipoprotein endocytosis by scavenger receptor SR-BII is clathrin-dependent and requires a carboxyl-terminal dileucine motif.
AuthorsEckhardt ER, Cai L, Shetty S, Zhao Z, Szanto A, Webb NR, Van der Westhuyzen DR
JournalJ Biol Chem
PubMed ID16368683
The high density lipoprotein (HDL) receptor Scavenger Receptor BII (SR-BII) is encoded by an alternatively spliced mRNA from the SR-BI gene and is expressed in various tissues. SR-BII protein differs from SR-BI only in the carboxyl-terminal cytoplasmic tail, which, as we showed previously, must contain a signal that confers predominant ... More
Fluorescence resonance energy transfer analysis of the antagonist- and partial agonist-occupied states of the cholecystokinin receptor.
AuthorsHarikumar KG, Miller LJ
JournalJ Biol Chem
PubMed ID15757907
Changes in receptor conformation are believed to be key for ligand-induced regulation of cellular signaling cascades. However, little information exists about specific conformations of a receptor. We recently applied fluorescence resonance energy transfer to determine distances from distinct points distributed over the surface and within the helical bundle of the ... More
Targeting of scavenger receptor class B type I by synthetic amphipathic alpha-helical-containing peptides blocks lipopolysaccharide (LPS) uptake and LPS-induced pro-inflammatory cytokine responses in THP-1 monocyte cells.
AuthorsBocharov AV, Baranova IN, Vishnyakova TG, Remaley AT, Csako G, Thomas F, Patterson AP, Eggerman TL
JournalJ Biol Chem
PubMed ID15199068
Human scavenger receptor class B type I, CLA-1, mediates lipopolysaccharide (LPS) binding and internalization (Vishnyakova, T. G., Bocharov, A. V., Baranova, I. N., Chen, Z., Remaley, A. T., Csako, G., Eggerman, T. L., and Patterson, A. P. (2003) J. Biol. Chem. 278, 22771-22780). Because one of the recognition motifs in ... More
Binding and internalization of lipopolysaccharide by Cla-1, a human orthologue of rodent scavenger receptor B1.
AuthorsVishnyakova TG, Bocharov AV, Baranova IN, Chen Z, Remaley AT, Csako G, Eggerman TL, Patterson AP
JournalJ Biol Chem
PubMed ID12651854
Scavenger receptor, class B, type I (SR-BI) mediates selective uptake of high density lipoprotein (HDL) cholesteryl ester. SR-BI recognizes HDL, low density lipoprotein (LDL), exchangeable apolipoproteins, and protein-free lipid vesicles containing negatively charged phospholipids. Lipopolysaccharides (LPS) are highly glycosylated anionic phospholipids contributing to septic shock. Despite significant structural similarities between ... More
A membrane-proximal basic domain and cysteine cluster in the C-terminal tail of CCR5 constitute a bipartite motif critical for cell surface expression.
AuthorsVenkatesan S, Petrovic A, Locati M, Kim YO, Weissman D, Murphy PM
JournalJ Biol Chem
PubMed ID11514564
We examined the structural requirements for cell surface expression, signaling, and human immunodeficiency virus co-receptor activity for the chemokine receptor, CCR5. Serial C-terminal truncation of CCR5 resulted in progressive loss of cell surface expression; mutants truncated at the 317th position and shorter were not detected at the cell surface. Alanine ... More
Trafficking of spontaneously endocytosed MHC proteins.
AuthorsChiu I, Davis DM, Strominger JL
JournalProc Natl Acad Sci U S A
PubMed ID10570178
Class I MHC protein primarily presents endogenous antigen but also may present exogenous antigen. Here, we investigated the intracellular pathway of spontaneously internalized class I MHC protein by confocal microscopy. beta(2)-microglobulin (beta(2)m), labeled with a single fluorophore, was exchanged at the surface of B cell transfectants to specifically mark cell ... More
Normal human kidney HLA-DR-expressing renal microvascular endothelial cells: characterization, isolation, and regulation of MHC class II expression.
AuthorsMuczynski KA, Ekle DM, Coder DM, Anderson SK
JournalJ Am Soc Nephrol
PubMed ID12707403
Human, but not murine, renal peritubular and glomerular capillaries constitutively express class II major histocompatibility (MHC) proteins at high levels in normal human kidney. Expression of class II proteins on renal microvascular endothelial cells (RMEC) makes it available to circulating lymphocytes and imparts a surveillance capacity to RMEC for controlling ... More
Annexin 2 binding to phosphatidylinositol 4,5-bisphosphate on endocytic vesicles is regulated by the stress response pathway.
AuthorsHayes MJ, Merrifield CJ, Shao D, Ayala-Sanmartin J, Schorey CD, Levine TP, Proust J, Curran J, Bailly M, Moss SE
JournalJ Biol Chem
PubMed ID14734570
Annexin 2 is a Ca(2+)-binding protein that has an essential role in actin-dependent macropinosome motility. We show here that macropinosome rocketing can be induced by hyperosmotic shock, either alone or synergistically when combined with phorbol ester or pervanadate. Rocketing was blocked by inhibitors of phosphatidylinositol-3-kinase(s), p38 mitogen-activated protein (MAP) kinase, ... More
Distinct Mechanisms for Visual and Motor-Related Astrocyte Responses in Mouse Visual Cortex.
AuthorsSlezak M, Kandler S, Van Veldhoven PP, Van den Haute C, Bonin V, Holt MG
JournalCurr Biol
PubMed ID31495587
'Astrocytes are a major cell type in the mammalian nervous system, are in close proximity to neurons, and show rich Ca'
Liquid and Hydrogel Phases of PrP
AuthorsKostylev MA, Tuttle MD, Lee S, Klein LE, Takahashi H, Cox TO, Gunther EC, Zilm KW, Strittmatter SM
JournalMol Cell
PubMed ID30401430
Protein phase separation by low-complexity, intrinsically disordered domains generates membraneless organelles and links to neurodegeneration. Cellular prion protein (PrP
Arabidopsis FLL2 promotes liquid-liquid phase separation of polyadenylation complexes.
AuthorsFang X, Wang L, Ishikawa R, Li Y, Fiedler M, Liu F, Calder G, Rowan B, Weigel D, Li P, Dean C
JournalNature
PubMed ID31043738
An important component of cellular biochemistry is the concentration of proteins and nucleic acids in non-membranous compartments
Absolute quantification of cohesin, CTCF and their regulators in human cells.
AuthorsHolzmann J, Politi AZ, Nagasaka K, Hantsche-Grininger M, Walther N, Koch B, Fuchs J, Dürnberger G, Tang W, Ladurner R, Stocsits RR, Busslinger GA, Novák B, Mechtler K, Davidson IF, Ellenberg J, Peters JM
JournalElife
PubMed ID31204999
The organisation of mammalian genomes into loops and topologically associating domains (TADs) contributes to chromatin structure, gene expression and recombination. TADs and many loops are formed by cohesin and positioned by CTCF. In proliferating cells, cohesin also mediates sister chromatid cohesion, which is essential for chromosome segregation. Current models of ... More
Toxic Tau Oligomers Modulated by Novel Curcumin Derivatives.
AuthorsLo Cascio F, Puangmalai N, Ellsworth A, Bucchieri F, Pace A, Palumbo Piccionello A, Kayed R
JournalSci Rep
PubMed ID31831807
The pathological aggregation and accumulation of tau, a microtubule-associated protein, is a common feature amongst more than 18 different neurodegenerative diseases that are collectively known as tauopathies. Recently, it has been demonstrated that the soluble and hydrophobic tau oligomers are highly toxic in vitro due to their capacity towards seeding ... More