Ethanol, 70% (AMR-E505-1) - FAQs

I want to use an Alexa Fluor Antibody Labeling kit to label my antibody. The antibody has histidine and glycine in the stock solution. Will this be a problem?

Yes. The reactive dye in the kit reacts with primary amine groups on histidine and glycine. This can result in less labeling of the antibody. The histidine and glycine must be removed, such as with dialysis or a desalting column. Other components that can interfere include BSA, gelatin, and Tris buffers.

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