Characterization of a single molecule DNA switch in free solution.
AuthorsWhite SS, Li H, Marsh RJ, Piper JD, Leonczek ND, Nicolaou N, Bain AJ, Ying L, Klenerman D
JournalJ Am Chem Soc
PubMed ID16939265
'We have studied a donor-acceptor fluorophore-labeled DNA switch where the acceptor is Alexa-647, a carbocyanine dye, in solution at the single molecule level to elucidate the fluorescence switching mechanism. The acceptor, which is in an initial high fluorescence trans state, undergoes a photoisomerization reaction resulting in two additional states during ... More
Ultrasensitive coincidence fluorescence detection of single DNA molecules.
AuthorsLi H, Ying L, Green JJ, Balasubramanian S, Klenerman D
JournalAnal Chem
PubMed ID12705600
We have detected individual DNA molecules labeled with two different fluorophores in solution by using two-color excitation and detection of coincidence fluorescence bursts. The confocal volumes of the two excitation lasers were carefully matched so that the volume overlap was 30% of the total confocal volume illuminated. This method greatly ... More