Goat anti-Mouse IgM (Heavy chain) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 546 - FAQs

I have a goat anti-mouse Alexa Fluor anti-IgM secondary antibody. Will it label my IgG primary antibody as well?

Not likely. With any secondary antibody, you need to consider what other isotypes and species they are cross-adsorbed against. If it is cross-adsorbed against other species or specific chains, cross-reactivity should be minimal or none. Our goat anti-IgM secondary antibodies have been cross-adsorbed against IgG and therefore should exhibit minimal binding to IgG.

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