Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555 - FAQs

3 个常见问题解答

我有两种来自于同一宿主种属的一抗，我希望用它们来标记同一种细胞，该如何选择二抗？

如果这两种一抗来自不同亚型（如小鼠IgG1和IgG2a），则可选择该亚型特异性的二抗。另一种办法是通过抗体标记试剂盒、活性染料或定制标记，直接与一抗进行偶联反应。

What storage conditions would you recommend for secondary antibodies conjugated with fluorophores or HRP?

We recommend storing secondary antibodies conjugated with fluorophores or HRP at 4 degrees C as we have found that storage at -20 degrees C and the associated continuous freeze/thaw cycles can reduce the performance of fluorescent dyes. Storage at 4 degrees C improves fluorophore stability without affecting antibody performance.
You can also prepare small aliquots to avoid freeze/thaw cycles and store them at -20 degrees C, as with each freeze/thaw step the performance of the antibody would go down.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

I have two primary antibodies from the same host species that I wish to use to label the same cells. How do I choose secondary antibodies?

If the two primary antibodies are from different isotypes (such as mouse IgG1 and IgG2a), there are isotype-specific secondary antibodies to choose from. The other option is to make direct conjugates of the primary antibodies through the use of antibody label kits, reactive dyes, or custom conjugations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.