Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594 - FAQs

3 个常见问题解答

我有两种来自于同一宿主种属的一抗，我希望用它们来标记同一种细胞，该如何选择二抗？

如果这两种一抗来自不同亚型（如小鼠IgG1和IgG2a），则可选择该亚型特异性的二抗。另一种办法是通过抗体标记试剂盒、活性染料或定制标记，直接与一抗进行偶联反应。

Do you offer an alternative for the discontinued 3-Germ Layer ICC Kit (Cat. No. A25538)?

We do not have a direct alternative for the discontinued 3-Germ Layer ICC Kit (Cat. No. A25538). However, we do have alternative primary and secondary antibodies, as well as reagents, that can be used for trilineage differentiation. Please note that we have not internally validated the use of all these reagents together.

We recommend the following primary antibodies:

Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4 (asm-1)) (Cat. No. MA5-11547)

alpha-Fetoprotein Monoclonal Antibody (AFP3) (Cat. No. 14-6583-80)

beta-3 Tubulin Monoclonal Antibody (2G10) (Cat. No. MA1-118)

We recommend the following secondary antibodies:

Goat anti-Mouse IgG2a Cross-Adsorbed, Alexa Fluor 555 (Cat. No. A21137)

Goat anti-Mouse IgG2a Cross-Adsorbed, Alexa Fluor 594 (Cat. No. A21135)

https://www.thermofisher.com/antibody/product/Goat-anti-Mouse-IgG1-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-21121

We recommend using the following reagents:

https://www.thermofisher.com/order/catalog/product/88-8824-00

NucBlue Fixed Cell ReadyProbes Reagent (DAPI) (Cat. No. R37606)

Blocker BSA (Cat. No. 37520)

DPBS (10X), no calcium, no magnesium (Cat. No. 14200075)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have two primary antibodies from the same host species that I wish to use to label the same cells. How do I choose secondary antibodies?

If the two primary antibodies are from different isotypes (such as mouse IgG1 and IgG2a), there are isotype-specific secondary antibodies to choose from. The other option is to make direct conjugates of the primary antibodies through the use of antibody label kits, reactive dyes, or custom conjugations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.