Sensitive two-color whole-mount in situ hybridizations using digoxygenin- and dinitrophenol-labeled RNA probes.
AuthorsLong S, Rebagliati M,
JournalBiotechniques
PubMed ID11911652
Immunohistochemical localization of somatostatin receptor SST2A in the rat pancreas.
AuthorsHunyady B, Hipkin RW, Schonbrunn A, Mezey E
JournalEndocrinology
PubMed ID9165058
Somatostatin (SRIF) acts on specific membrane receptors to inhibit exocrine and endocrine pancreatic functions. Five SRIF receptor genes have been cloned, producing six receptor proteins (sst-s). We used a recently developed antibody to localize the sst2A splice variant in the rat pancreas. Western blots identified the sst2A receptor as an ... More
Immunohistochemical signal amplification by catalyzed reporter deposition and its application in double immunostaining.
AuthorsHunyady B, Krempels K, Harta G, Mezey E
JournalJ Histochem Cytochem
PubMed ID8985127
The biotinyl-tyramide substrate of the horseradish peroxidase enzyme has been recently introduced to amplify immunohistochemical signals. We applied either fluorochromeor biotin-conjugated tyramine to improve the detection of different antigens in sections of rat stomach, pancreas, and hypothalamus. A ten- to 100-fold increase in staining efficiency was achieved, depending on the ... More