Inhibition of eukaryotic DNA replication by geminin binding to Cdt1.
AuthorsWohlschlegel JA, Dwyer BT, Dhar SK, Cvetic C, Walter JC, Dutta A
JournalScience
PubMed ID11125146
'In all eukaryotic organisms, inappropriate firing of replication origins during the G2 phase of the cell cycle is suppressed by cyclin-dependent kinases. Multicellular eukaryotes contain a second putative inhibitor of re-replication called geminin. Geminin is believed to block binding of the mini-chromosome maintenance (MCM) complex to origins of replication, but ... More
Cell cycle. License withheld--geminin blocks DNA replication.
AuthorsLygerou Z, Nurse P
JournalScience
PubMed ID11188727
A cell ensures that its genome is replicated only once during its division cycle through a process called licensing. In an enlightening Perspective, Lygerou and Nurse explain how binding of the Geminin protein to Cdt1 blocks the binding of licensing factors to chromatin, inhibiting the onset of S phase (Wohlschlegel ... More
Improved cervical smear assessment using antibodies against proteins that regulate DNA replication.
AuthorsWilliams GH, Romanowski P, Morris L, Madine M, Mills AD, Stoeber K, Marr J, Laskey RA, Coleman N
JournalProc Natl Acad Sci U S A
PubMed ID9843993
Carcinoma of the cervix is one of the most common malignancies. Papanicolaou (Pap) smear tests have reduced mortality by up to 70%. Nevertheless their interpretation is notoriously difficult with high false-negative rates and frequently fatal consequences. We have addressed this problem by using affinity-purified antibodies against human proteins that regulate ... More