The properties of keratin intermediate filaments (IFs) have been studied after transfection with green fluorescent protein (GFP)-tagged K18 and/or K8 (type I/II IF proteins). GFP-K8 and -K18 become incorporated into tonofibrils, which are comprised of bundles of keratin IFs. These tonofibrils exhibit a remarkably wide range of motile and dynamic ... More
Gaf-1, a gamma -SNAP-binding protein associated with the mitochondria.
AuthorsChen D, Xu W, He P, Medrano EE, Whiteheart SW
JournalJ Biol Chem
PubMed ID11278501
The role of alpha/beta-SNAP (Soluble NSF Attachment Protein) in vesicular trafficking is well established; however, the function of the ubiquitously expressed gamma-SNAP remains unclear. To further characterize the cellular role of this enigmatic protein, a two-hybrid screen was used to identify new, gamma-SNAP-binding proteins and to uncover potentially novel functions ... More
Oxidative stress affects cytoskeletal structure and cell-matrix interactions in cells from an ocular tissue: the trabecular meshwork.
AuthorsZhou L, Li Y, Yue BY
JournalJ Cell Physiol
PubMed ID10395288
The trabecular meshwork (TM) is a specialized eye tissue that regulates the aqueous humor outflow and controls intraocular pressure. Cells in this tissue are essential for maintenance of the outflow system. Disturbance of the TM cell status by insults such as oxidative stress may lead to elevation of the intraocular ... More
Distinction between HLA class I-positive and -negative cervical tumor subpopulations by multiparameter DNA flow cytometry.
AuthorsCorver WE, Koopman LA, Mulder A, Cornelisse CJ, Fleuren GJ
JournalCytometry
PubMed ID10942899
BACKGROUND: The study of the molecular-genetic basis of heterogeneity of HLA class I expression in solid tumors is hampered by the lack of reliable rapid cell-by-cell isolation techniques. Hence, we studied the applicability of a flow cytometric approach (Corver et al.: Cytometry 2000;39;96-107). METHODS: Cells were isolated from five fresh ... More
Structural and biological consequences of increased vimentin expression in simple epithelial cell types.
AuthorsAndreoli JM, Trevor KT
JournalCell Motil Cytoskeleton
PubMed ID8674130
Cytoskeletal intermediate filaments (IFs) constitute a diverse family of proteins whose members are expressed in tissue-specific patterns. Although vimentin IFs are normally restricted to mesenchyme, a variety of cell types express vimentin alone or together with cell-specific IFs during growth, differentiation, and neoplasia. In this study, we have investigated the ... More