The N termini of focal adhesion kinase family members regulate substrate phosphorylation, localization, and cell morphology.
AuthorsDunty JM, Schaller MD
JournalJ Biol Chem
PubMed ID12223467
The focal adhesion kinase (FAK) and cell adhesion kinase beta (CAKbeta, PYK2, CADTK, RAFTK) are highly homologous FAK family members, yet clearly have unique roles in the cell. Comparative analyses of FAK and CAKbeta have revealed intriguing differences in their activities. These differences were investigated further through the characterization of ... More
Features of medullary thymic epithelium implicate postnatal development in maintaining epithelial heterogeneity and tissue-restricted antigen expression.
AuthorsGillard GO, Farr AG
JournalJ Immunol
PubMed ID16670287
'Although putative thymic epithelial progenitor cells have been identified, the developmental potential of these cells, the extent of medullary thymic epithelium (mTEC) heterogeneity, and the mechanisms that mediate the expression of a wide range of peripheral tissue-restricted Ags (TRAs) by mTECs remain poorly defined. Here we have defined several basic ... More
Deficiency of the zinc finger protein ZPR1 causes defects in transcription and cell cycle progression.
AuthorsGangwani L
JournalJ Biol Chem
PubMed ID17068332
'The zinc finger protein ZPR1 is present in both the cytoplasm and nucleoplasm. Cell cycle analysis demonstrates that ZPR1 undergoes major changes in subcellular distribution during proliferation. ZPR1 is diffusely localized throughout the cell during the G(1) and G(2)/M phases of the cell cycle. In contrast, ZPR1 redistributes to the ... More
Specific recruitment of human cohesin to laser-induced DNA damage.
AuthorsKim JS, Krasieva TB, LaMorte V, Taylor AM, Yokomori K
JournalJ Biol Chem
PubMed ID12228239
'Cohesin is a conserved multiprotein complex that plays an essential role in sister chromatid cohesion. During interphase, cohesin is required for the establishment of cohesion following DNA replication. Because cohesin mutants resulted in increased sensitivity to DNA damage, a role for cohesin in DNA repair was also suggested. However, it ... More
Ephrin-B1 is critical in T-cell development.
AuthorsYu G, Mao J, Wu Y, Luo H, Wu J
JournalJ Biol Chem
PubMed ID16476740
'Eph kinases are the largest family of receptor tyrosine kinases, and their ligands, ephrins (EFNs), are also cell surface molecules. In this study, we investigated the role of EFNB1 and the Ephs it interacts with (collectively called EFNB1 receptors) in mouse T-cell development. In the thymus, CD8 single positive (SP) ... More
The histone deacetylase inhibitor trichostatin A alters the pattern of DNA replication origin activity in human cells.
AuthorsKemp MG, Ghosh M, Liu G, Leffak M,
JournalNucleic Acids Res
PubMed ID15653633
'Eukaryotic chromatin structure limits the initiation of DNA replication spatially to chromosomal origin zones and temporally to the ordered firing of origins during S phase. Here, we show that the level of histone H4 acetylation correlates with the frequency of replication initiation as measured by the abundance of short nascent ... More
Intensity calibration and automated cell cycle gating for high-throughput image-based siRNA screens of mammalian cells.
AuthorsPoon SS, Wong JT, Saunders DN, Ma QC, McKinney S, Fee J, Aparicio SA,
JournalCytometry A
PubMed ID18698634
'High-content microscopic screening systems are powerful tools for extracting quantitative multiparameter measures from large number of cells under numerous conditions. These systems perform well in applications that monitor the presence of objects, but lack in their ability to accurately estimate object intensities and summarize these findings due to variations in ... More
Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2'-deoxyuridine antibodies.
The 5-bromo-2'-deoxyuridine (BrdU) labeling of cells followed by antibody staining has been the standard method for direct measurement of cells in the S-phase. Described is an improved method for the detection of S-phase cell cycle progression based upon the application of click chemistry, the copper(I)-catalyzed variant of the Huisgen [3+2] ... More
Angiogenesis selectively requires the p110alpha isoform of PI3K to control endothelial cell migration.
AuthorsGraupera M, Guillermet-Guibert J, Foukas LC, Phng LK, Cain RJ, Salpekar A, Pearce W, Meek S, Millan J, Cutillas PR, Smith AJ, Ridley AJ, Ruhrberg C, Gerhardt H, Vanhaesebroeck B,
JournalNature
PubMed ID18449193
Phosphoinositide 3-kinases (PI3Ks) signal downstream of multiple cell-surface receptor types. Class IA PI3K isoforms couple to tyrosine kinases and consist of a p110 catalytic subunit (p110alpha, p110beta or p110delta), constitutively bound to one of five distinct p85 regulatory subunits. PI3Ks have been implicated in angiogenesis, but little is known about ... More
Nuclear autoantigenic sperm protein (NASP), a linker histone chaperone that is required for cell proliferation.
A multichaperone nucleosome-remodeling complex that contains the H1 linker histone chaperone nuclear autoantigenic sperm protein (NASP) has recently been described. Linker histones (H1) are required for the proper completion of normal development, and NASP transports H1 histones into nuclei and exchanges H1 histones with DNA. Consequently, we investigated whether NASP ... More
Remodeling of the intestine during metamorphosis of Xenopus laevis.
AuthorsSchreiber AM, Cai L, Brown DD
JournalProc Natl Acad Sci U S A
PubMed ID15738398
Thyroid hormone controls remodeling of the tadpole intestine during the climax of amphibian metamorphosis. In 8 days, the Xenopus laevis tadpole intestine shortens in length by 75%. Simultaneously, the longitudinal muscle fibers contract by about the same extent. The radial muscle fibers also shorten as the diameter narrows. Many radial ... More
Activation of protease-activated receptor-1 triggers astrogliosis after brain injury.
AuthorsNicole O, Goldshmidt A, Hamill CE, Sorensen SD, Sastre A, Lyuboslavsky P, Hepler JR, McKeon RJ, Traynelis SF
JournalJ Neurosci
PubMed ID15858058
We have studied the involvement of the thrombin receptor [protease-activated receptor-1 (PAR-1)] in astrogliosis, because extravasation of PAR-1 activators, such as thrombin, into brain parenchyma can occur after blood-brain barrier breakdown in a number of CNS disorders. PAR1-/- animals show a reduced astrocytic response to cortical stab wound, suggesting that ... More
Multiple rows of cells behind an epithelial wound edge extend cryptic lamellipodia to collectively drive cell-sheet movement.
AuthorsFarooqui R, Fenteany G
JournalJ Cell Sci
PubMed ID15585576
The mechanism by which epithelial, endothelial and other strongly cell-cell adhesive cells migrate collectively as continuous sheets is not clear, even though this process is crucial for embryonic development and tissue repair in virtually all multicellular animals. Wound closure in Madin-Darby canine kidney (MDCK) epithelial cell monolayers involves Rac GTPase-dependent ... More