Signaling through extracellular signal-regulated kinase is required for spermatogonial proliferative response to stem cell factor.
AuthorsDolci S, Pellegrini M, Di Agostino S, Geremia R, Rossi P
JournalJ Biol Chem
PubMed ID11502745
'In vitro addition of stem cell factor (SCF) to c-kit-expressing A(1)-A(4) spermatogonia from prepuberal mice stimulates their progression into the mitotic cell cycle and significantly reduces apoptosis in these cells. SCF addition results in a transient activation of extracellular signal-regulated kinases (Erk)1/2 as well as of phosphatidylinositol 3-kinase (PI3K)-dependent Akt ... More
Cyclin D3 accumulation and activity integrate and rank the comitogenic pathways of thyrotropin and insulin in thyrocytes in primary culture.
AuthorsVan Keymeulen A, Bartek J, Dumont JE, Roger PP
JournalOncogene
PubMed ID10602491
The proliferation of most normal cells depends on the synergistic interaction of several growth factors and hormones, but the cell cycle basis for this combined requirement remains largely uncharacterized. We have addressed the question of the requirement for insulin/IGF-1 also observed in many cell culture systems in the physiologically relevant ... More
Cyclin D1 is dispensable for G1 control in retinoblastoma gene-deficient cells independently of cdk4 activity.
AuthorsLukas J, Bartkova J, Rohde M, Strauss M, Bartek J
JournalMol Cell Biol
PubMed ID7739541
To elucidate the regulator-versus-target relationship in the cyclin D1/cdk4/retinoblastoma protein (pRB) pathway, we examined fibroblasts from RB-1 gene-deficient and RB-1 wild-type littermate mouse embryos (ME) and in human tumor cell lines that differed in the status of the RB-1 gene. The RB+/+ and RB-/- ME fibroblasts expressed similar protein levels ... More